Immunochromatography Using Colloidal Gold−Antibody Probe for the Detection of Atrazine in Water Samples

Shim, Won‐Bo; Yang, Zhengyou; Kim, Jiyoung; Choi, Jin-Gil; Je, Jung-Hyun; Kang, Sang‐Woo; Kolosova, A. S.; Eremin, Sergei A.; Chung, Duck-Hwa

doi:10.1021/jf0620057

Cited by 97 publications

(76 citation statements)

References 18 publications

(17 reference statements)

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“…Sample pads were treated according to Shim et al (27), with some modifications. Cellulose fiber sample pads (Millipore), 1.5 by 0.5 cm, were dipped into the sample pad buffer (50 mM borate buffer [pH 7.2], 5% sucrose, 0.5% Tween 20, 5% dextran, and 0.1% skim milk) and then dried at 50°C.…”

Section: Methodsmentioning

confidence: 99%

“…(28), and the rrl gene (482 bp), which is specific for Leptospira genus (29). The primers used in this experiment were specific for the flaB gene (L-flaB-F1 [5=-TCTCACCGTTCTCTAAAGTTCAAC-3=] and L-flaB-R1 [5=-CTGAATTCGGTTTCATATTTGCC-3=]) (27) and for the rrl gene (rrlF [5=-GACCCGAAGCCTGTCGAG-3=] and rrlR [5=-GCCATGCTTAGTCCCGATTAC-3=]) (29) of Leptospira spp. flaB was amplified under the following conditions: 40 cycles of denaturing at 94°C for 20 s, annealing at 50°C for 30 s, extension at 72°C for 60 s, and final extension at 72°C for 5 min.…”

Section: Serratia Marcescens J1 J5mentioning

confidence: 99%

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Development of Immunochromatography-Based Methods for Detection of Leptospiral Lipopolysaccharide Antigen in Urine

Widiyanti¹,

Koizumi²,

Fukui³

et al. 2013

Clin. Vaccine Immunol.

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Leptospirosis is an infectious disease caused by the spirochete bacteria Leptospira spp. and is commonly found throughout the world. Diagnosis of leptospirosis performed by culture and microscopic agglutination tests is laborious and time-consuming. Therefore, we aimed to develop a novel immunochromatography (ICG)-based method for detecting Leptospira antigen in the urine of patients and animals. We used the 1H6 monoclonal antibody (MAb), which is specific to the lipopolysaccharide (LPS) that is common among Leptospira spp. The MAb was coupled to 40-nm-diameter colloidal gold, and the amounts of labeled antibody and immobilized antibody were 23 g and 2 g per test, respectively. Several strains of Leptospira and other bacterial species were used to evaluate the sensitivities and specificities of the assays we developed. The detection limit of the assays was 10 6 cells/ml when disrupted whole bacterial cells were used. The assays were Leptospira specific since they did not cross-react with non-Leptospira bacteria used in the study. Application of diagnostic assays was done on the urine samples of 46 Leptospira-infected hamsters, 44 patients with suspected leptospirosis, and 14 healthy individuals. Pretreatment of the urine samples by boiling and centrifugation (for ultrafiltration and concentration) eliminated nonspecific reactions that occurred in the assay. The sensitivity and specificity of the ICG-based lateral flow assay (LFA) were 89% and 87%, respectively, which were higher than those of the dipstick assay, which were 80% and 74%, respectively. In summary, this ICG-based LFA can be used as an alternative diagnostic assay for leptospirosis. Further development is still necessary to improve the assay.

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Section: Methodsmentioning

confidence: 99%

Section: Serratia Marcescens J1 J5mentioning

confidence: 99%

Development of Immunochromatography-Based Methods for Detection of Leptospiral Lipopolysaccharide Antigen in Urine

Widiyanti¹,

Koizumi²,

Fukui³

et al. 2013

Clin. Vaccine Immunol.

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“…Some authors have successfully described LFDs for aflatoxins that work well with corn [18], grains and feedstuffs [25,26] and milk [27], but require strip treatment before use. A new LFD for aflatoxins that did not require special pre-treatment was developed by Masinde et al [19] and has been commercialised as the Aflatoxin Quicktest™ by QuickTest Technologies.…”

Section: Poisoning -From Specific Toxic Agents To Novel Rapid and Simmentioning

confidence: 99%

The Aflatoxin QuicktestTM—A Practical Tool for Ensuring Safety in Agricultural Produce

Sánchez‐Bayo¹,

Almeida²,

Williams³

et al. 2017

Poisoning - From Specific Toxic Agents to Novel Rapid and Simplified Techniques for Analysis

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Contamination of corn, peanuts, milk and dairy products with aflatoxins is a worldwide problem, particularly in subtropical regions where the climatic conditions are ideal for the growth of Aspergillus flavus, the fungi that produces these toxins. Developing countries have major difficulties in marketing these products abroad due to the stringent international regulations concerning this carcinogenic toxin. Adding to the problem is the analytical cost involved in monitoring the produce, which require sophisticated instrumentation and qualified personnel, neither of which are available for field testing. The development of a rapid Aflatoxin Quicktest™ provides an effective, reliable and cheaper option for screening levels of aflatoxin above the regulatory thresholds in such produce. The test consists of a lateral flow device (LFD) coated with antibodies specific to aflatoxin B1, although it detects other aflatoxins (i.e. G and M) with high cross-reactivity. Its high sensitivity allows analysis of these toxins in the range 2-40 μg/kg of sample in 15 minutes, plus the time for extraction, which varies among different products. Quantification of the test results is done using a Quick Reader, by comparing the readings of individual tests against a standard curve of the analytes in the same manner as it is done with any other analytical equipment. A validation study was carried out using peanuts from Australia and peanuts and corn from Timor-Leste to assess the performance of the Aflatoxin Quicktest™. Results obtained with the LFD showed a good correlation with the standard analytical measurements by HPLC-fluorescence (r 2 above 0.90 for all cases), indicating the Aflatoxin Quicktest™ is capable of measuring levels of aflatoxins accurately and reliably. Given their ease of use, low cost and fast processing time, the Aflatoxin Quicktest™ can be used for screening agricultural produce in countries that cannot afford the costly alternative of using specialised personnel and equipment.

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“…It was reported that Helicobacter pylori in the urine was detected using an immunochromatographic assay (Graham and Reddy, 2001). Atrazine in water samples (Shim et al, 2006) and theophylline enzyme in whole blood samples were also detected by an immunochromatographic method (Habib et al, 1987). Bikker et al (2007) reported the detection of sulfur mustard adducts in human callus by phage antibodies.…”

Section: Specificity Study Of Laminin-5 γ γ γ γ γ 2 Degradation Usingmentioning

confidence: 99%

An immunochromatographic assay to detect reduced level of laminin‐5 γ2 in sulfur mustard‐exposed normal human epidermal keratinocytes

Jin

Ray²,

Ray

2008

J of Applied Toxicology

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The need for reliable methods to detect the nature and extent of poisoning with chemical warfare agents is evident from the recent threat of use of these agents in warfare and terrorist attacks. Sulfur mustard (SM; 2,2'-dichlorodiethyl sulfide) is an alkylating vesicant agent, which has been used as a chemical weapon in various conflicts during the 20th century. The injuries resulting from SM-exposure are mainly characterized by epithelial damage of the tissues through which it is absorbed, i.e. skin, eye and the respiratory tract. Proteins in the skin mostly affected by SM-exposure are laminin-5 and integrin alpha6beta4. Laminin-5 constitutes the anchoring filaments and binds the transmembrane protein integrin alpha6beta4. Recent studies have shown that SM alkylation causes a significant reduction of laminin-5, disruption of alpha6beta4 integrin and decreases the expression of integrin alpha6 and beta4 subunits, therefore, leads to destabilization of dermal-epidermal attachments and potentiates vesication. This study established a unique immunochromatographic detection method (strip assay) to detect the degradation of laminin-5 in SM-exposed NHEK (normal human epidermal keratinocytes) extract. This method may serve as a rapid SM-exposure diagnostic/screening procedure that could be applied directly to skin extracts of individuals who have supposedly been exposed to SM.

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Immunochromatography Using Colloidal Gold−Antibody Probe for the Detection of Atrazine in Water Samples

Cited by 97 publications

References 18 publications

Development of Immunochromatography-Based Methods for Detection of Leptospiral Lipopolysaccharide Antigen in Urine

Development of Immunochromatography-Based Methods for Detection of Leptospiral Lipopolysaccharide Antigen in Urine

The Aflatoxin QuicktestTM—A Practical Tool for Ensuring Safety in Agricultural Produce

An immunochromatographic assay to detect reduced level of laminin‐5 γ2 in sulfur mustard‐exposed normal human epidermal keratinocytes

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