Immunochemical detection of penicillins by using biohybrid magnetic particles

Broto, Marta; Matas, Sonia; Babington, Ruth; Marco, M.‐Pilar; Galvé, Roger

doi:10.1016/j.foodcont.2014.11.043

Cited by 20 publications

(7 citation statements)

References 30 publications

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“…The preparation of the bioconjugates for estradiol (6E2 4 BSA) and DA (DA 5 BSA) was performed using the following procedures. Briefly, a solution of N, N'dicyclohexylcarbodiimide (DCC; 50 μmol) in anhydrous DMF (50 μL) was added to a solution of the hapten (100 µL) in the same solvent followed by a solution of Nhydroxysuccinimide (NHS, 25 μmols, 50 µL) also in DMF [34]. The mixture was stirred for approximately 3 hours at RT until a white precipitate appeared.…”

Section: 4bioconjugate Competitorsmentioning

confidence: 99%

Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples

Sanchís

Salvador

Campbell

et al. 2018

Talanta

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The development of a fluorescent multiplexed microarray platform able to detect and quantify a wide variety of pollutants in seawater is reported. The microarray platform has been manufactured by spotting 6 different bioconjugate competitors and it uses a cocktail of 6 monoclonal or polyclonal antibodies raised against important families of chemical pollutants such as triazine biocide (i.e. Irgarol 1051®), sulfonamide and chloramphenicol antibiotics, polybrominated diphenyl ether flame-retardant (PBDE, i.e. BDE-47), hormone (17β-estradiol), and algae toxin (domoic acid). These contaminants were selected as model analytes, however, the platform developed has the potential to detect a broader group of compounds based on the cross-reactivity of the immunoreagents used. The microarray chip is able to simultaneously determine these families of contaminants directly in seawater samples reaching limits of detection close to the levels found in contaminated areas (Irgarol 1051®, 0.19 ± 0,06 µg L; sulfapyridine, 0.17 ± 0.07 µg L; chloramphenicol, 0.11 ± 0.03 µg L; BDE-47, 2.71 ± 1.13 µg L; 17β-estradiol, 0.94 ± 0.30 µg L and domoic acid, 1.71 ± 0.30 µg L). Performance of the multiplexed microarray chip was assessed by measuring 38 blind spiked seawater samples containing either one of these contaminants or mixtures of them. The accuracy found was very good and the coefficient of variation was < 20% in all the cases. No sample pre-treatment was necessary, and the results could be obtained in just 1 h 30 min. The microarray shows high sample throughput capabilities, being able to measure simultaneously more than 68 samples and screen them for a significant number of chemical contaminants of interest in environmental screening programs.

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Section: 4bioconjugate Competitorsmentioning

confidence: 99%

Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples

Sanchís

Salvador

Campbell

et al. 2018

Talanta

Self Cite

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“…Another ELISA method has been developed for the detection of penicillin in the milk sample. This assay is based on open-ringed forms of penicillin which were treated by hybrid magnetic particles which permit the detection of two types of penicillin simultaneously (Broto, Matas, Babington, Marco, & Galve, 2015). Additionally, a specific and sensitive ELISA has been established to detect the 1-amino-hydantoin (AHD) residues in fish, shrimp, pork, and chicken samples, a specific antibody was prepared based on the monoclonal antibody knowledge against AHD.…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

119

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Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions of an analytical method in the evaluation of food and environmental protection. This review offers an overview of the preparation of antibodies and the currently established immuneassays for the antibiotic residues detection, including enzymelinked immunosorbent assay, fluorescence immunoassay, radioimmunoassay, colloidal gold immunoassay, and chemiluminescence immunoassay. Furthermore, it will elaborate on the future perspective on the performance and improvement of these assays for the determination of antibiotic residues in food samples. These assays include a simple sample preparation and high-sensitive detection that offer a significant assurance for the screening of antibiotic residues.

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“…Antibiotics have been widely used to treat animal diseases and promote animal growth , . Penicillin, as one kind of β‐lactam antibiotics, has been most widely applied because of its broad spectrum antibiotic activity . However, the use of antibiotics will lead to the problem of antibiotic residues in food or in discharge to the aquatic environment, which has aroused great concern because antibiotics can cause allergic reactions in sensitive individuals.…”

Section: Introductionmentioning

confidence: 99%

“…[13,14] Penicillin, as one kind of βlactam antibiotics, has been most widely applied because of its broad spectrum antibiotic activity. [15] However, the use of antibiotics will lead to the problem of antibiotic residues in food or in discharge to the aquatic environment, which has * J. zigzag arrays bridged by L ligands. Notably, the L ligands take the (η 4 , μ 4 ) coordination fashion in 1 but (η 2 , μ 2 ) binding mode in 2 and 3.…”

Section: Introductionmentioning

confidence: 99%

Halide‐directed Assembly of Mercury(II) Coordination Polymers for Electrochemical Biosensing Toward Penicillin

Xue

Chen

2020

Zeitschrift anorg allge chemie

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Three 1D/2D HgII coordination polymers (CPs), namely {[Hg3(L)2Cl6](H2O)}n (1), [Hg(L)Br2]n (2), and [Hg(L)I2]n (3) were prepared by assembly of 3,4‐bis(3‐pyridyl)‐5‐(4‐pyridyl)‐1,2,4‐triazole (L) with different inorganic HgII salts (i.e. HgCl2, HgBr2 or HgI2). Single‐crystal X‐ray diffraction analysis reveals two types of bimetallic subunits [(Hg)2C6N4] and [(Hg)2C12N6] in CP 1, which are further extended to a 2D coordination network. Both of CPs 2 and 3 show 1D zigzag arrays bridged by L ligands. Notably, the L ligands take the (η4, μ4) coordination fashion in 1 but (η2, μ2) binding mode in 2 and 3. The structural differences of CPs 1–3 indicate that the L ligand can adjust its coordination fashions to meet the requirements of HgII centers, relying on the presence of distinct halide anions. In addition, 1 can be applied to fabricate an electrochemical biosensor for the detection of penicillin.

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Immunochemical detection of penicillins by using biohybrid magnetic particles

Cited by 20 publications

References 30 publications

Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples

Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples

Current advances in immunoassays for the detection of antibiotics residues: a review

Halide‐directed Assembly of Mercury(II) Coordination Polymers for Electrochemical Biosensing Toward Penicillin

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