Immunoblot Analysis of the Serological Response in Systemic Candidosis

Matthews, Ruth; Burnie, James; Tabaqchali, Soad

doi:10.1016/s0140-6736(84)91618-0

Cited by 156 publications

(87 citation statements)

References 16 publications

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“…In mice, enolase stimulates both humoral and cellular immune responses. The glycolytic enzyme, enolase catalyzes the reversible dehydration of 2-phospho-D-glycerate to high-energy phosphoenolpyruvate (Matthews et al, 1984). Characterization of antigenic components in cytoplasmic extracts of C. albicans that were recognized in sera from patients with disseminated candidiasis has been reported (Strockbine et al, 1984).…”

Section: Fungal Infection Of Vagina Is Sometimes Called a Thrush Canmentioning

confidence: 99%

Culture and Identification of Candida Albicans from Vaginal Ulcer and Separation of Enolase on SDS-PAGE

Bhavan¹,

Rajkumar²,

Radhakrishnan³

et al. 2010

IJB

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Candida albicans were isolated from patients with clinical symptoms of vaginal ulcer. Culture test for vaginal swab and scrapings were conducted on Sabouraud's dextrose broth and Sabouraud's dextrose agar plate respectively. Hichrome candida agar culture was used for differential identification of Candida. Smears from vaginal scrapings were prepared for gram staining. The suspected strain of Candida was inoculated on corn meal agar medium for chlamydospore formation. The suspected strain of Candida was inoculated in human serum for germ tube formation. Carbohydrate assimilation and fermentation tests were also conducted. The selected Candida colony was inoculated in YEPD medium for subculture and the cultured organism was harvested. The organisms were homogenized, centrifuged and the supernatant was filtered. The filtrate was extracted in chloroform. The extract was centrifuged and the aqueous phase was dialyzed. The dialyzed crude enolase was subjected to SDS-PAGE. The Sabouraud's dextrose broth inoculated with vaginal swab showed turbid growth. The scraping from vagina showed typical smooth creamy white colonies with a characteristic yeast odour on Sabouraud's dextrose agar plate. On Hichrome candida agar the Candida growth appeared as glistening green colored. Gram stained smears from vaginal scraps showed appearance of fungus as yeast January, 2010 85 budding. On corn meal agar the suspected Candida growth showed the formation of large, highly refractive, thick walled terminal chlamydospores. Germ tubes were seen as long tube like projections extending from the yeast cells on human serum inoculated with suspected strain of Candida. The carbohydrate assimilation tests were positive for dextrose, maltose, sucrose, galactose, xylose and trehalose, and negative for lactose, melibiose, ellobiose, inositol, reffinose and dulcitol. The carbohydrate fermentation tests showed positive for dextrose, maltose, galactose and trehalose, and negative for sucrose and lactose. SDS-PAGE for enolase from C. albicans showed a single polypeptide band of around 46 -48 kDa. International Journal of Biology

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Section: Fungal Infection Of Vagina Is Sometimes Called a Thrush Canmentioning

confidence: 99%

Culture and Identification of Candida Albicans from Vaginal Ulcer and Separation of Enolase on SDS-PAGE

Bhavan¹,

Rajkumar²,

Radhakrishnan³

et al. 2010

IJB

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“…Furthermore, Middleton et al [43] did not reveal an association or a correlation between a group with IBS benefiting from antifungal treatment and microbiological findings of yeasts in stools. Evidence for yeast as a causative factor for symptoms has rather been sought from indirect measurements that look for evidence of yeast metabolites in blood, breath or urine [5,6,10,12,[44][45][46], or that explore yeast allergy/sensitivity [12,44,[47][48][49][50][51][52][53].…”

Section: Diagnostic Testsmentioning

confidence: 99%

“…But the interpretation of test results is difficult, due to standardization problems with the extracts (different antigen quantity and quality from different Candida strains, grown on different growth media and antigens selected from different parts of the yeast), cross-reactivity between other yeasts and moulds and last not least different antibody responses (IgE, IgA, IgG, IgM). Not surprisingly, many of these tests do not distinguish patients from normal controls [48][49][50][51]. Measurement of a combination of antigen, antibody and immune complexes in the serum may be most valuable in the diagnosis of fungus related disease [52][53][54].…”

Section: Diagnostic Testsmentioning

confidence: 99%

Yeast metabolic products, yeast antigens and yeasts as possible triggers for irritable bowel syndrome

Santelmann

Howard²

2005

European Journal of Gastroenterology & Hepatology

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Many patients with irritable bowel syndrome (IBS) are disillusioned by the lack of efficacy of treatments and suffer from numerous symptoms not covered by the Rome criteria for IBS, as the current empirical treatment regimes fail to address these persistent debilitating 'IBS associated symptoms'. These symptoms are similar to other symptom complexes like chronic fatigue and the so called 'candida syndrome', and many seek help from alternative medicine. The possible role of Candida and yeasts in non-immune compromised individuals is disputed and is the subject of this review. Even if the involvement of yeasts in the aetiology of IBS still remains unclear, there is increasing evidence for yeasts being able to cause IBS-symptoms in sensitized patients via Candida products, antigens and cross-antigens. But more research is needed before antifungal treatment can be recommended as a first line treatment for IBS.

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“…The complexity of the C albicans antigenic profile is confirmed by the heterogeneity in antibody response to various antigenic determinants of C. albicans (58)(59)(60). Nevertheless, relatively little is known about the role of the various protein components of C albicans in eliciting either humoral or cell-mediated immune responses against the organism.…”

Section: Antigenic Variationmentioning

confidence: 99%

“…Recently, considerable effort has been directed towards an analysis of an immunodominant 47 kDa protein that may be important in eliciting a protective antibody. Despite the apparent heterogeneity in human antibody responses to C. albicans, 92% of patients with systemic candidiasis produce a response to an antigen of 47 kDa (58,99). Ability to produce and maintain a good antibody response to this antigen was associated with recovery; whereas fatal cases produced tittle or no antibody, or initially had good antibody titres which subsequently fell as their condition deteriorated (100).…”

Section: Role Of Antibodymentioning

confidence: 99%