Immunobiology of the reproductive tract in a female baboon

Abstract

The aim of this study was to assess the suitability of various antihuman antibodies directed against immunocomponent cells to identify components involved in cellular and humoral immune responses in the immune organs of a female baboon, and to use these reagents to analyze the immunobiology of its reproductive tract. A female baboon of reproductive age was euthanized in the luteal phase of the menstrual cycle, and samples of spleen, intestines, tonsil, lymph nodes, Fallopian tube, uterus, cervix, and vagina were removed. Tissues were either fixed in 10% unbuffered formaldehyde, Bouin’s fluid, or 95% ethanol containing 5% glacial acetic acid, and embedded in paraffin, or frozen unfixed. Frozen sections were then fixed in 100% acetone. Subsequently, tissue sections were reacted with the following antihuman antibodies directed against CD3, CD45RA, CD45RO, CD4, CD8, CD20, CD68, HLA‐DR, CD57, CD103, CD15, and TIA‐1: IgA, IgG, IgM, J‐chain, secretory component, and neutrophil elastase, using routine immunohistology techniques. Human tissues (spleen, small intestine, lymph node, and tonsil) were used as positive controls. All antihuman antibodies crossreacted with baboon tissues, except neutrophil elastase, CD15, CD45RO, CD57, and CD1A. The distribution of immune cells in the reproductive tract of the female baboon was comparable to that in the human and offers the potential for this primate to be used as a model for the study of human reproductive immunology. Am. J. Primatol. 53:47–54, 2001. © 2001 Wiley‐Liss, Inc.

Gender Differences in Human Immunodeficiency Virus Type 1-Specific CD8 Responses in the Reproductive Tract and Colon following Nasal Peptide Priming and Modified Vaccinia Virus Ankara Boosting

“…Antigen-specific IFN-␥ responses in the colon. Although the reproductive tracts of males and females both exhibit immunologic characteristics indicating they are tissues of the mucosal immune system (9,10,26,28,29,(40)(41)(42)46), the male and female reproductive tracts are obviously anatomically and functionally distinct. Therefore, we evaluated antigen-specific IFN-␥ responses in the colons of mice immunized with our prime-boost immunization strategies.…”

Gender Differences in Human Immunodeficiency Virus Type 1-Specific CD8 Responses in the Reproductive Tract and Colon following Nasal Peptide Priming and Modified Vaccinia Virus Ankara Boosting

“…Antigen-specific IFN-␥ responses in the colon. Although the reproductive tracts of males and females both exhibit immunologic characteristics indicating they are tissues of the mucosal immune system (9,10,26,28,29,(40)(41)(42)46), the male and female reproductive tracts are obviously anatomically and functionally distinct. Therefore, we evaluated antigen-specific IFN-␥ responses in the colons of mice immunized with our prime-boost immunization strategies.…”

Future Directions in Endometriosis Research

“…This would be very important to assess the validity of the Sampson hypothesis. Most monoclonal antibodies used in humans for ELISA and immunohistochemistry and primers used for RT‐PCR experiments also can be used to analyze with baboon tissues 42‐45…”

Thiazolidinediones, Agonisten des PPAR-γ-Rezeptors (peroxisomaler proliferatoraktivierter Rezeptor) reduzieren die peritoneale Makrophagenanzahl - Neue medikamentöse Therapieoptionen für die Inflammation und Infertilität bei Endometriose? -

“…Wir planen nun, die Anwendung eines Thiazolidinediones (z. B. Rosiglitazone oder Pioglitazone) bei Pavianen mit Endometriose zu erproben [11]. Menschenaffen sind leider die einzige Tierspezies, die an Endometriose leiden.…”

Gender Differences in Human Immunodeficiency Virus Type 1-Specific CD8 Responses in the Reproductive Tract and Colon following Nasal Peptide Priming and Modified Vaccinia Virus Ankara Boosting

“…Antigen-specific IFN-␥ responses in the colon. Although the reproductive tracts of males and females both exhibit immunologic characteristics indicating they are tissues of the mucosal immune system (9,10,26,28,29,(40)(41)(42)46), the male and female reproductive tracts are obviously anatomically and functionally distinct. Therefore, we evaluated antigen-specific IFN-␥ responses in the colons of mice immunized with our prime-boost immunization strategies.…”

Future Directions in Endometriosis Research

“…This would be very important to assess the validity of the Sampson hypothesis. Most monoclonal antibodies used in humans for ELISA and immunohistochemistry and primers used for RT‐PCR experiments also can be used to analyze with baboon tissues 42‐45…”

Thiazolidinediones, Agonisten des PPAR-γ-Rezeptors (peroxisomaler proliferatoraktivierter Rezeptor) reduzieren die peritoneale Makrophagenanzahl - Neue medikamentöse Therapieoptionen für die Inflammation und Infertilität bei Endometriose? -

“…Wir planen nun, die Anwendung eines Thiazolidinediones (z. B. Rosiglitazone oder Pioglitazone) bei Pavianen mit Endometriose zu erproben [11]. Menschenaffen sind leider die einzige Tierspezies, die an Endometriose leiden.…”

Gender Differences in Human Immunodeficiency Virus Type 1-Specific CD8 Responses in the Reproductive Tract and Colon following Nasal Peptide Priming and Modified Vaccinia Virus Ankara Boosting

“…Antigen-specific IFN-␥ responses in the colon. Although the reproductive tracts of males and females both exhibit immunologic characteristics indicating they are tissues of the mucosal immune system (9,10,26,28,29,(40)(41)(42)46), the male and female reproductive tracts are obviously anatomically and functionally distinct. Therefore, we evaluated antigen-specific IFN-␥ responses in the colons of mice immunized with our prime-boost immunization strategies.…”

Future Directions in Endometriosis Research

“…This would be very important to assess the validity of the Sampson hypothesis. Most monoclonal antibodies used in humans for ELISA and immunohistochemistry and primers used for RT‐PCR experiments also can be used to analyze with baboon tissues 42‐45…”

Thiazolidinediones, Agonisten des PPAR-γ-Rezeptors (peroxisomaler proliferatoraktivierter Rezeptor) reduzieren die peritoneale Makrophagenanzahl - Neue medikamentöse Therapieoptionen für die Inflammation und Infertilität bei Endometriose? -

“…Wir planen nun, die Anwendung eines Thiazolidinediones (z. B. Rosiglitazone oder Pioglitazone) bei Pavianen mit Endometriose zu erproben [11]. Menschenaffen sind leider die einzige Tierspezies, die an Endometriose leiden.…”