2022
DOI: 10.1590/0074-02760220086
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Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement

Abstract: BACKGROUND Angiostrongylus cantonensis is the etiological agent of neuroangiostrongyliasis in humans, which is developed in gastropods and vertebrate species, mainly rodents. Human transmission occurs through consumption of molluscs and paratenic hosts infected with L3, and the migration of larvae to the central nervous system causes eosinophilic meningitis. Laboratory diagnosis is based on molecular and immunological tests, using young or adult females as a source of antigens. However, these tests give positi… Show more

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Cited by 2 publications
(2 citation statements)
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“…The PCR assay allows for parasite DNA detection in CSF during the acute phase, meaning it can obtain a diagnosis faster than serological testing. PCR has now become the primary confirmatory diagnostic method for confirming A. cantonensis infection [ 4 6 , 8 , 9 ].…”
Section: Discussionmentioning
confidence: 99%
“…The PCR assay allows for parasite DNA detection in CSF during the acute phase, meaning it can obtain a diagnosis faster than serological testing. PCR has now become the primary confirmatory diagnostic method for confirming A. cantonensis infection [ 4 6 , 8 , 9 ].…”
Section: Discussionmentioning
confidence: 99%
“…Numerous techniques, including ELISA, immunoblot assays, gold immunochromatography, and rapid dot immunogold filtration assays, have been used for decades. However, these techniques may have limitations as the antibody may be undetectable in the early stages of infection [ 3 , 30 , 51 , 52 ]. Molecular diagnostic methods have been employed as a robust diagnostic tool based on PCR, real-time PCR, LAMP, and recombinase polymerase assay (RPA), which has the potential to improve AEM diagnosis by enabling highly sensitive detection of A. cantonensis DNA in a patient’s CSF, serum, or other materials [ 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 ].…”
Section: Epidemiology and Clinical Manifestations Of Human Angiostron...mentioning
confidence: 99%