Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)

Sushytskyi, Leonid; Lukáč, Pavol; Synytsya, Andriy; Bleha, Roman; Rajsiglová, Lenka; Capek, Peter; Pohl, Radek; Vannucci, Luca; Čopı́ková, Jana; Kaštánek, Petr

doi:10.1016/j.carbpol.2020.116588

Cited by 19 publications

(6 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polysaccharides from microalgae Parachlorella kessleri HY1 biomass were purified and isolated, and the immunomodulation effect of the purified extracts on melanoma-bearing C57Bl/6 mice was studied. 91 The polysaccharides could elicit immune responses by stimulating innate and adaptive immunity. The data revealed that polysaccharides isolated from Parachlorella kessleri could function as nonspecific immune stimulators that played an auxiliary role in the tumor immunotherapy process.…”

Section: Treatment Of Oxygen Deficiency Aggravated Diseasesmentioning

confidence: 99%

How Microalgae is Effective in Oxygen Deficiency Aggravated Diseases? A Comprehensive Review of Literature

Cui¹,

Su²,

Wei³

et al. 2022

IJN

View full text Add to dashboard Cite

Hypoxia can aggravate the conditions of many oxygen-deficiency-aggravated diseases (ODAD), such as cancer, ischemic heart disease, and chronic wounds. Photosynthetic microalgae can alleviate the hepatotoxicity of the local microenvironment by producing oxygen. In addition, microalgae extracts have antitumor, anti-inflammatory, antibacterial, and antioxidant effects. These properties make them attractive candidates for developing methods to treat ODAD. Although researchers have exploited the advantages of microalgae and developed a variety of microalgae-based biomaterials to treat ODAD, a comprehensive review of this topic has not been presented previously. Therefore, in this review, we summarize the development and progress made in the field of developing microalgae-based biomaterials toward the treatment of ODAD. The challenges and prospects of this field are also discussed.

show abstract

Section: Treatment Of Oxygen Deficiency Aggravated Diseasesmentioning

confidence: 99%

How Microalgae is Effective in Oxygen Deficiency Aggravated Diseases? A Comprehensive Review of Literature

Cui¹,

Su²,

Wei³

et al. 2022

IJN

View full text Add to dashboard Cite

show abstract

“…The insoluble part after extractions with cold water was used for further extractions with hot water under reflux (100 • C). The extraction was performed in three portions five times in distilled water (1000 mL each portion) for approximately 8 h. The extracts were used for obtaining of purified fraction F2 by the same way as described above for F1; further purification was performed using anion exchange preparative chromatography [36,70]. Chromatographic fractionation of F2 (66.67 mg) led to obtaining of three sub-fractions assigned as F2a, F2b, and F2c.…”

Section: Preparative Proceduresmentioning

confidence: 99%

Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure

et al. 2022

Self Cite

View full text Add to dashboard Cite

In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum. Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L−1 sodium hydroxide. The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy. The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan. Mannogalactan predominated in the cold water extract, and β-d-glucan was the main product of the hot water extract. The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction. The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone. The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-d-glucans.

show abstract

“…Water soluble polysaccharides have been isolated and purified from the biomass of the green alga Parachlorella kessleri HY1, and their immunomodulatory activities were evaluated on splenocytes from homogenized spleens of healthy and melanoma bearing C57Bl/6 mice. The polysaccharide tested with immuno-spot assay increased the production of INF-γ in the melanoma cells [ 42 ]. In another study, the sulpho-glycolipidic fraction of the red microalgae Porphiridium cruentum has been tested [ 43 ].…”

Section: Marine Microorganismsmentioning

confidence: 99%

Marine Compounds for Melanoma Treatment and Prevention

Montuori

Capalbo

Lauritano

2022

IJMS

View full text Add to dashboard Cite

Melanoma is considered a multifactorial disease etiologically divided into melanomas related to sun exposure and those that are not, but also based on their mutational signatures, anatomic site, and epidemiology. The incidence of melanoma skin cancer has been increasing over the past decades with 132,000 cases occurring globally each year. Marine organisms have been shown to be an excellent source of natural compounds with possible bioactivities for human health applications. In this review, we report marine compounds from micro- and macro-organisms with activities in vitro and in vivo against melanoma, including the compound Marizomib, isolated from a marine bacterium, currently in phase III clinical trials for melanoma. When available, we also report active concentrations, cellular targets and mechanisms of action of the mentioned molecules. In addition, compounds used for UV protection and melanoma prevention from marine sources are discussed. This paper gives an overview of promising marine molecules which can be studied more deeply before clinical trials in the near future.

show abstract

Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)

Cited by 19 publications

References 69 publications

How Microalgae is Effective in Oxygen Deficiency Aggravated Diseases? A Comprehensive Review of Literature

How Microalgae is Effective in Oxygen Deficiency Aggravated Diseases? A Comprehensive Review of Literature

Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure

Marine Compounds for Melanoma Treatment and Prevention

Contact Info

Product

Resources

About