Immunization with Biodegradable Nanoparticles Efficiently Induces Cellular Immunity and Protects against Influenza Virus Infection

Abstract

The ability of vaccines to induce T cell responses is crucial for preventing diseases caused by viruses or bacteria. Nanoparticles (NPs) are considered an efficient tool for inducing potent immune responses. In this study, we describe a novel vaccination approach with biodegradable calcium phosphate (CaP) NPs that serve as carrier of immunoactive TLR9 ligand (CpG) combined with a viral Ag from the influenza A virus hemagglutinin. Functionalized CaP NPs were efficiently taken up by dendritic cells in vivo and elicited a potent T cell–mediated immune response in immunized mice with high numbers of IFN-γ–producing CD4+ and CD8+ effector T cells. Most importantly, both i.p. and intranasal immunization with these NPs offered protection in a mouse model of influenza virus infection. This study demonstrates the great potential of CaP NPs as a novel vaccination tool that offers substantial flexibility for several infection models.

Vaccine Technologies Against Avian Influenza: Current Approaches and New Directions

“…284 305 Robust T cell-mediated immunity against influenza virus infection can also be induced using biodegradable nanoparticles. 306 In this regard, it has become clear that novel nanovaccine delivery platforms can be employed to induce protective humoral and cellmediated immunity against a variety of pathogens including influenza virus.…”

Biological targeting with nanoparticles: state of the art

“…To understand these factors, both experimental (i.e., analytical) as well as simulation techniques are applied [12,13]. Depending on the envisioned application, nanoparticles are adapted to deliver different kinds of therapeutic agents like small drug molecules [14,15] and biomolecules (peptides, proteins, nucleic acids) [16][17][18]. Furthermore, metallic nanoparticles (often gold) [19][20][21][22][23][24][25][26][27] and magnetic nanoparticles (often magnetite, Fe 3 O 4 , or maghemite, Fe 2 O 3 ) [28][29][30] are used for tumor targeting, followed by local heat application ("tumor thermotherapy"), [31,32] and also for imaging in the body.…”

Colonic gene silencing using siRNA-loaded calcium phosphate/PLGA nanoparticles ameliorates intestinal inflammation in vivo

“…Among other delivery strategies, calcium phosphate (CaP) nanoparticles have proven to be efficient in biomedicine [15][16][17][18][19] e.g. as carriers of small molecules across the cell membrane [20,21]. CaP nanoparticles have a high affinity to nucleic acids, and the crystal growth of CaP can be inhibited on the nanometer scale by stabilization and simultaneous functionalization with nucleic acids [22,23].…”