Immune serum–activated human macrophages coordinate with eosinophils to immobilize <i>Ascaris suum</i> larvae

Coakley, Gillian; Volpe, Beatrice; Bouchery, Tiffany; Shah, Kathleen; Butler, Alana; Geldhof, Peter; Hatherill, Mark; Horsnell, William G. C.; Bieren, Julia Esser‐von; Harris, Nicola L.

doi:10.1111/pim.12728

Cited by 12 publications

(11 citation statements)

References 89 publications

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“…Moreover, using the same methodology, anti-Ascaris IgG 4 seropositive serum has been shown to react with live larvae. 58 Both strategies strengthen our findings and suggest that we are truly measuring antibodies toward Ascaris. Exposure to Anisakis simplex due to fish consumption could be a potential cause of cross-reactivity; however, a study of Norwegian blood donors showed a prevalence of almost 0 for IgE sensitization toward Anisakis.…”

Section: Discussionsupporting

confidence: 72%

Ascaris exposure and its association with lung function, asthma, and DNA methylation in Northern Europe

Jõgi

Kitaba

Storaas

et al. 2022

Journal of Allergy and Clinical Immunology

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Background: Ascaris infections, with a worldwide prevalence above 10%, can cause respiratory pathology. However, longterm effects on lung function in humans are largely unknown. Objective: We investigated the associations of Ascaris exposure with lung function, asthma, and DNA methylation. Methods: Serum Ascaris IgG antibodies were measured in 671 adults aged 18 to 47 years (46% women) from Aarhus, Bergen, and Tartu RHINESSA study centers. Seropositivity was defined as IgG above the 90th percentile. Linear and logistic regressions were used to analyze Ascaris seropositivity as associated with lung function and asthma, adjusted for age, height, and smoking and clustered by center. DNA methylation in blood was profiled by a commercial methylation assay. Results: Ascaris seropositivity was associated with lower FEV 1 (2247 mL; 95% CI, 2460, 234) and higher odds for asthma (adjusted odds ratio, 5.84; 95% CI, 1.67, 20.37) among men but not women, also after further adjusting for house dust mite sensitivity, consistent across study centers. At a genome-wide level, Ascaris exposure was associated with 23 differentially methylated sites in men and 3 in women. We identified hypermethylation of the MYBPC1 gene, which can regulate airway muscle contraction. We also identified genes linked to asthma pathogenesis such as CRHR1 and GRK1, as well as a differentially methylated region in the PRSS22 gene linked to nematode infection. Conclusion: Ascaris exposure was associated with substantially lower lung function and increased asthma risk among men. Seropositive participants had sex-specific differences in DNA methylation compared to the unexposed, thus suggesting that exposure may lead to sex-specific epigenetic changes associated with lung pathology. (J Allergy Clin Immunol 2021;nnn:nnnnnn.)

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Section: Discussionsupporting

confidence: 72%

Ascaris exposure and its association with lung function, asthma, and DNA methylation in Northern Europe

Jõgi

Kitaba

Storaas

et al. 2022

Journal of Allergy and Clinical Immunology

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“…In contrast, Arg1-mediated trapping of N. brasiliensis larvae by macrophages required the IgE/ FceR-driven activation of IL-4producing basophils [4]. Recently, an important role for antibody-activated macrophages in larval trapping has also been shown for the human parasite Ascaris lumbricoides [6]. However, in contrast to macrophage-mediated immunity to the rodent parasite H. polygyrus, antibodymediated trapping of Ascaris larvae was greatly enhanced in the presence of eosinophils, which were recruited by immune serum-activated human macrophages [2,6].…”

Section: Antibody-mediated Macrophage Functions In Host Defense and R...mentioning

confidence: 99%

“…However, based on their exceptional plasticity, macrophages have evolved multiple strategies to efficiently combat helminth parasites and to limit parasite-induced tissue damage (Figure 1). Macrophage-mediated host defense against helminth parasites largely relies on the activation of an alternatively activated macrophage (AAM) phenotype, which can trap and/ or kill helminth larvae or stimulate the expulsion of adult parasites [2][3][4][5][6][7]. While AAM-mediated helminth trapping prevents extensive injury of infected tissues [2,8], AAM also actively contribute to tissue repair by producing growth factors, chemokines and building blocks for collagen synthesis [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Macrophage regulation & function in helminth infection

Lechner

Bohnacker

Bieren

2021

Seminars in Immunology

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“…eosinophil, Giardia muris, IgA, ILC3, parasite, protozoa, Th17 nematodes. 9,11,12 However, the healthy small intestine harbours high numbers of eosinophils, which support Peyer's patches (PP) development and mucus production 13 and promote homeostatic IgA antibody class switching (CS). Eosinophils also support IgAproducing plasma cells in the small intestinal lamina propria (siLP) at steady state, and during intestinal nematode and Toxoplasma gondii infection.…”

Section: Introductionmentioning

confidence: 99%

Eosinophils are dispensable for the regulation of IgA and Th17 responses in Giardia muris infection

Yordanova

Lamatsch

Kühl

et al. 2020

Parasite Immunology

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The small intestinal protozoan parasite Giardia lamblia is a major cause of food-and water-borne diarrhoeal disease worldwide. 1,2 Mice deficient in B cells, IgA, the polymeric immunoglobulin receptor A (pIgR) or IL-17A fail to control infections with G lamblia or the murine species G muris, emphasizing the importance of humoral immunity and Th17 cells for the control of giardiasis. 2-7 In line with those findings, we have previously observed differential Th17 activity, IgA production and regulatory T-cell responses associated with differences in susceptibility of inbred mouse lines towards G muris infection. 8 Over the last decade, eosinophils were shown to regulate both IgA production and Th17 activity at steady state and during infection (reviewed in 9,10). Eosinophils typically expand during helminth infection and may trap and damage migrating larvae of parasitic

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Immune serum–activated human macrophages coordinate with eosinophils to immobilize Ascaris suum larvae

Cited by 12 publications

References 89 publications

Ascaris exposure and its association with lung function, asthma, and DNA methylation in Northern Europe

Ascaris exposure and its association with lung function, asthma, and DNA methylation in Northern Europe

Macrophage regulation & function in helminth infection

Eosinophils are dispensable for the regulation of IgA and Th17 responses in Giardia muris infection

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