Immune response and serum bactericidal activity against Brucella ovis elicited using a short immunization schedule with the polymeric antigen BLSOmp31 in rams

Díaz, Ana Carolina; Clausse, María; Paolicchi, Fernando Alberto; Fiorentino, Marí­a Andrea; Ghersi, Giselle; Zylberman, Vanesa; Goldbaum, Fernando A.; Estein, Silvia Marcela

doi:10.1016/j.vetimm.2013.04.003

Cited by 19 publications

(18 citation statements)

References 23 publications

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“…BLS WT is a powerful immunogen, [21][22][23][24][25][26][27] and hence we decided to evaluate the capacity of BLS DRKE and the pentameric variants to elicit humoral immune responses, taking BLS WT as a reference. BALB/cJ mice were injected intraperitoneally with 10 mg of each of the proteins.…”

Section: Bls Drke Retains Immunological Properties Of Bls Wtmentioning

confidence: 99%

“…15,18 BLS plays a key role in the Brucella infectious process, 19 shows high thermal and chemical stability 20 and elicits a strong immunogenic response in different animal models. [21][22][23][24][25][26][27] Its disordered N-termini (rst 8-10 residues of all chains) allow to genetically fuse polypeptides without affecting its structural properties, giving rise to chimeric constructs. BLS has been successfully used as a structural scaffold for vaccine design displaying a multiple variety of epitopes, [28][29][30][31] ranging from linear peptides to protein domains and to entire proteins.…”

Section: Introductionmentioning

confidence: 99%

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Asymmetric bifunctional protein nanoparticles through redesign of self-assembly

et al. 2019

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Section: Bls Drke Retains Immunological Properties Of Bls Wtmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Asymmetric bifunctional protein nanoparticles through redesign of self-assembly

et al. 2019

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“…Subsequently, several notable achievements in the realm of the organism's characteristics, virulence, pathogenicity, diagnosis and development of vaccines have been reported (Doganay and Aygen, 2003;Corbel, 2006;Osman et al, 2016). Brucella melitensis is generally considered to involve infection through ingestion, inhalation and through direct contact with broken skin and often severely terminates in septicaemia with isolation of the organism from multiple organs (Grilló et al, 2012;Díaz et al, 2013;Osman et al, 2017). Livestock farmers, veterinarians and laboratory workers are at risk of being affected by this organism due to their constant and close contact with animals and animal related materials (Samadi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Penang has an average rainfall of 340 mm of rain and experiences hot and humid weather throughout the year with two monsoon seasons; the north-east monsoon from November to March and the south-east monsoon from May to September (http://www.met.gov.my). Malaysia has a relatively small goat population size with estimated number of 482,280 heads (Malaysia: Livestock Population, 2009-2013.…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and Economic Impact Assessment of Caprine Brucellosis in Penang State, Malaysia: A Retrospective Analysis

Zaki¹,

Osman²,

Nordin³

et al. 2018

American Journal of Animal and Veterinary Sciences

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Brucella melitensis is a zoonotic pathogen affecting a wide range of hosts with economic significance in the animal industry worldwide. The disease is characterized by abortion, still birth, reduced milk production, weak foetus and infertility mainly in small ruminant populations. There is a wide distribution of the disease among domesticated species and the trends of the disease in goats have not yet been described in Penang state, Malaysia. In this study we describe the distribution, pattern and trend of B. melitensis in Penang state based on serological data obtained from nationwide B. melitensis serosurveillance activities in goat populations. A total of 13,724 goats were sampled within the period of study (2012)(2013)(2014), of which 3.4% (95% CI; 3.35-3.45) goats tested positive for B. melitensis infection. The district of Seberang Perai Utara had the highest animal seroprevalence of 95.5% (95% CI; 94.2-96.8). The seroprevalence varied over type of the breeds and the period of study and generally increased in 2014. Seropositive animals clustered around Boer and Katjang breeds with the prevalence of 54% (95% CI; 51.8-56.2) and 38% (95% CI; 35.8-40.2), respectively. The months of March, August and September, illustrated the highest seroprevalences of 44 (95% CI; 13% (95% CI;.2) and 14% (95% CI; 11.3-16.7), respectively. A noticeable variation was observed in annual-level economic loses, but the seroprevalence remained significantly high in 2014 (>80%). Considering the lack of information on the epidemiology of caprine brucellosis in peninsular Malaysia, this report could contribute to the ongoing area-wise national brucellosis eradication program.

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“…The ideal vaccine against B. ovis must prevent infection and clinical signs or at least reduce the risk of infection and transmission (11). Rev-1, a live attenuated Brucella melitensis strain, is the most commonly used vaccine against B. melitensis, and it also protects against B. ovis (12).…”

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Protection Provided by an Encapsulated Live Attenuated ΔabcBAStrain of Brucella ovis against Experimental Challenge in a Murine Model

Silva

Macêdo

Silva

et al. 2015

Clin. Vaccine Immunol.

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dThis study aimed to evaluate the Brucella ovis ⌬abcBA strain as a vaccine candidate in the murine model. BALB/c mice were subcutaneously or intraperitoneally immunized with a single dose or three doses of the B. ovis ⌬abcBA strain and then were challenged with wild-type B. ovis. Single or multiple immunizations provided only mild protection, with significantly smaller numbers of wild-type B. ovis CFU in the livers of immunized mice but not in the spleens. Encapsulation of B. ovis ⌬abcBA significantly improved protection against experimental challenges in both BALB/c and C57BL/6 mice. Furthermore, immunization with encapsulated B. ovis ⌬abcBA markedly prevented lesions in the spleens and livers of experimentally challenged mice. These results demonstrated that the encapsulated B. ovis ⌬abcBA strain confers protection to mice; therefore, this strain has potential as a vaccine candidate for rams. Brucella ovis is a facultative, intracellular, Gram-negative coccobacillus (1) that causes economic losses due to its potential to induce reproductive failure in sheep (2, 3). The most important lesions caused by B. ovis in rams are epididymitis and seminal vesiculitis (4), and B. ovis occasionally causes abortion in ewes (5, 6).In spite of attempts to develop safe, effective vaccines (7-10), there is no available vaccine specifically against B. ovis. The ideal vaccine against B. ovis must prevent infection and clinical signs or at least reduce the risk of infection and transmission (11). Rev-1, a live attenuated Brucella melitensis strain, is the most commonly used vaccine against B. melitensis, and it also protects against B. ovis (12). However, the Rev-1 strain retains pathogenic potential, so it is capable of infecting humans. It may cause abortions in ruminants, it may interfere with serological tests, and it is resistant to streptomycin (13,14).Mice have been extensively used as a model for brucellosis, which is very important due to the limitations of experimentation with larger animals (15-18). The murine model has allowed studies for the development of safe, effective brucellosis vaccines (15,19,20).Inactivation of a predicted abcEDCBA-encoded ATP-binding cassette (ABC) transporter of B. ovis (21), which is located in B. ovis pathogenicity island 1 (BOPI-1), results in strong attenuation of this pathogen in the murine model (22). Although the substrate of this ABC transporter is not known, it is required for expression of B. ovis virulence proteins, including Omp31, Sod, and the virB-encoded type IV secretion system (23), which is required for B. ovis intracellular survival and in vivo persistence (17). Furthermore, a B. ovis ⌬abcBA strain is capable of triggering humoral and cellular immune responses like the wild-type strain in rams; importantly, however, the mutant strain is not excreted in semen and urine (24). Therefore, this mutant strain has great potential as an attenuated vaccine strain. However, considering that the ⌬abcBA mutant strain is attenuated at very early stages during infection, resulting in p...

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Immune response and serum bactericidal activity against Brucella ovis elicited using a short immunization schedule with the polymeric antigen BLSOmp31 in rams

Cited by 19 publications

References 23 publications

Asymmetric bifunctional protein nanoparticles through redesign of self-assembly

Asymmetric bifunctional protein nanoparticles through redesign of self-assembly

Epidemiology and Economic Impact Assessment of Caprine Brucellosis in Penang State, Malaysia: A Retrospective Analysis

Protection Provided by an Encapsulated Live Attenuated ΔabcBAStrain of Brucella ovis against Experimental Challenge in a Murine Model

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