Immune response against the biomaterials used in 3D bioprinting of organs

Elalouf, Amir

doi:10.1016/j.trim.2021.101446

Cited by 20 publications

(22 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, it is such a pity that clinical trials concerning the use of bioprinting for skin conditions in humans are so rare. One of the insurmountable obstacles is human immune rejections against bioprinted transplants, which are mediated by numerous cell types such as B-cells, T-cells, neutrophils, dendritic cells, natural killer cells, macrophages, and molecular signals such as cytokines, antibodies, and reactive radical species [ 186 ]. Notably, human immune responses are against both biomaterials and embedded cells after the transplantation.…”

Section: Discussionmentioning

confidence: 99%

Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing

Wang

Yuan

Yao

et al. 2022

Bioactive Materials

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing

Wang

Yuan

Yao

et al. 2022

Bioactive Materials

View full text Add to dashboard Cite

“…Three-dimensional bioprinting was developed on the basis of 3D printing. Cells and scaffolds are generally made of polymers and extruded from a nozzle and cultured in 3D ( Elalouf, 2021 ; Jamee et al, 2021 ). Many organs, such as bone ( Arrigoni et al, 2017 ; Kim et al, 2021 ; Alcala-Orozco et al, 2022 ), lung ( Hu et al, 2021 ), and skin ( Gao et al, 2021c ), have already been generated by 3D bioprinting, showing plenty of potential in the future.…”

Section: Advanced Models For Ex Vivo Non Studiesmentioning

confidence: 99%

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Zhang

Rong

Bian

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Increasing population is suffering from neurological disorders nowadays, with no effective therapy available to treat them. Explicit knowledge of network of neurons (NoN) in the human brain is key to understanding the pathology of neurological diseases. Research in NoN developed slower than expected due to the complexity of the human brain and the ethical considerations for in vivo studies. However, advances in nanomaterials and micro-/nano-microfabrication have opened up the chances for a deeper understanding of NoN ex vivo, one step closer to in vivo studies. This review therefore summarizes the latest advances in lab-on-chip microsystems for ex vivo NoN studies by focusing on the advanced materials, techniques, and models for ex vivo NoN studies. The essential methods for constructing lab-on-chip models are microfluidics and microelectrode arrays. Through combination with functional biomaterials and biocompatible materials, the microfluidics and microelectrode arrays enable the development of various models for ex vivo NoN studies. This review also includes the state-of-the-art brain slide and organoid-on-chip models. The end of this review discusses the previous issues and future perspectives for NoN studies.

show abstract

“…An uncontrolled inflammatory response to implants results in fibrous encapsulation, which can lead to implant failure [4,5]. Foreign body response (FBR) caused by implanted biomaterials seriously impedes the function of the implants and is a major obstacle to the development of implantable tissues and medical devices [6].…”

Section: Introductionmentioning

confidence: 99%

Ionically annealed zwitterionic microgels for bioprinting of cartilaginous constructs

Surman,

Asadikorayem,

Weber

et al. 2024

Biofabrication

View full text Add to dashboard Cite

Foreign body response (FBR) is a pervasive problem for biomaterials used in tissue engineering. Zwitterionic hydrogels have emerged as an effective solution to this problem, due to their ultra-low fouling properties, which enable them to effectively inhibit FBR in vivo. However, no versatile zwitterionic bioink that allows for high resolution extrusion bioprinting of tissue implants has thus far been reported. In this work, we introduce a simple, novel method for producing zwitterionic microgel bioink, using alginate methacrylate (AlgMA) as crosslinker and mechanical fragmentation as a microgel fabrication method. Photocrosslinked hydrogels made of zwitterionic carboxybetaine acrylamide (CBAA) and sulfobetaine methacrylate (SBMA) are mechanically fragmented through meshes with aperture diameters of 50 and 90 µm to produce microgel bioink. The bioinks made with both microgel sizes showed excellent rheological properties and were used for high-resolution printing of objects with overhanging features without requiring a support structure or support bath. The AlgMA crosslinker has a dual role, allowing also for both primary photocrosslinking of the bulk hydrogel as well as secondary ionic crosslinking of produced microgels, to quickly stabilize the printed construct in a calcium bath and to produce a microporous scaffold. Scaffolds showed ~20% porosity, and they supported viability and chondrogenesis of encapsulated human primary chondrocytes. Finally, a meniscus model was bioprinted, to demonstrate the bioink’s versatility at printing large, cell-laden constructs which are stable for further in vitro culture to support tissue maturation. This easy and scalable strategy of producing zwitterionic microgel bioink for high resolution extrusion bioprinting allows for direct cell encapsulation in a microporous scaffold and has potential for in vivo biocompatibility due to the zwitterionic nature of the bioink.

show abstract

Immune response against the biomaterials used in 3D bioprinting of organs

Cited by 20 publications

References 111 publications

Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing

Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Ionically annealed zwitterionic microgels for bioprinting of cartilaginous constructs

Contact Info

Product

Resources

About