Immune-metabolic profiling of anorexic patients reveals an anti-oxidant and anti-inflammatory phenotype

Omodei, Daniela; Pucino, Valentina; Labruna, Giuseppe; Procaccini, Claudio; Galgani, Mario; Perna, Francesco; Caprio, Carmela De; Marone, Gianni; Fontana, Luigi; Contaldo, Franco; Pasanisi, Fabrizio; Matarese, Giuseppe; Sacchetti, Lucia

doi:10.1016/j.metabol.2014.10.025

Cited by 37 publications

(26 citation statements)

References 34 publications

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“…Bioenergetic dysfunction has been demonstrated in various disease states including Alzheimer's disease [37], type 2 diabetes [38] and anorexia nervosa [39]. Each of these disorders has also been associated with low vitamin D status [40], [41], [42].…”

Section: Discussionmentioning

confidence: 99%

“…There is growing evidence that circulating factors can mediate bioenergetic function of PBMCs [18]. Numerous studies have harnessed PBMC's as a potential tool to determine the inflammatory and metabolic status in a variety of different disease states, including sepsis [19], neurodegeneration [20], rheumatic disease [21], obesity [22], [23], cardiovascular disease [24], diabetes mellitus [25], [26], and anorexia nervosa [27], all of which have been linked to mitochondrial dysfunction or altered bioenergetics. These studies in immune cells are consistent with a large body of work suggesting altered mitochondrial respiratory capacity and function in tissues classically studied such as skeletal muscle, liver, islet cells, and the myocardium [28], [29], [30], [31].…”

Section: Introductionmentioning

confidence: 99%

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Prevailing vitamin D status influences mitochondrial and glycolytic bioenergetics in peripheral blood mononuclear cells obtained from adults

et al. 2016

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BackgroundCirculating peripheral blood mononuclear cells (PBMCs) are exposed to metabolic and immunological stimuli that influence their functionality. We hypothesized that prevailing vitamin D status [25(OH)D] would modulate the bioenergetic profile of PBMCs derived from humans.Materials and methods38 participants (16 males, 22 females) ranging in body fat from 14–51% were studied. PBMCs were isolated from whole blood, counted and freshly seeded for bioenergetic analysis using the Seahorse XFe96 flux analyser. Whole body energy metabolism via indirect calorimetry, body composition by dual-energy X-ray absorptiometry, and relevant clinical biochemistry were measured. Data was analysed based on 25(OH)D cut-offs of <50 nmol/L (Group 1, n=12), 50–75 nmol/L (Group 2, n=15) and ≥75 nmol/L (Group 3, n=11). A multivariate general linear model adjusting for age, fat mass, fat-free mass, parathyroid hormone and insulin sensitivity was used.ResultsThere were significant differences in cellular mitochondrial function between groups. Group 1 had significantly higher basal respiration (p=0.001), non-mitochondrial respiration (p=0.009), ATP production (p=0.001), proton leak (p=0.018), background glycolysis (p=0.023) and glycolytic reserve (p=0.039) relative to either Group 2 or Group 3; the latter two did not differ on any measures. There were no differences in bioenergetic health index (BHI), resting metabolic rates and systemic inflammatory markers between groups.ConclusionsInadequate vitamin D status adversely influenced bioenergetic parameters of PBMCs obtained from adults, in a pattern consistent with increased oxidative metabolism and activation of these cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevailing vitamin D status influences mitochondrial and glycolytic bioenergetics in peripheral blood mononuclear cells obtained from adults

et al. 2016

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“…Clinical experience verifies that refeeding is agony and feels terrible. Finally, acutely ill individuals with AN do not exhibit classic sickness behaviours characteristic of the presence of inflammatory processes (21,22). Each of these is in stark contrast to the majority of individuals in the world who regularly battle with the opposite tendencies in their quest for weight loss.…”

Section: Anorexia Nervosamentioning

confidence: 99%

Towards a science of eating disorders: Replacing myths with realities: The fourth Birgit Olsson lecture

Bulik

2015

Nordic Journal of Psychiatry

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“…These include obsessive–compulsive disorder (OCD) (2, 3), anorexia nervosa (AN) (4), tic disorders (5), attention deficit hyperactivity disorder (ADHD) (6), major depressive disorder (7), and bipolar disorder (8). The precise role immune mechanisms play in these disorders remains to be determined.…”

Section: Introductionmentioning

confidence: 99%

Temporal Association of Certain Neuropsychiatric Disorders Following Vaccination of Children and Adolescents: A Pilot Case–Control Study

et al. 2017

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BackgroundAlthough the association of the measles, mumps, and rubella vaccine with autism spectrum disorder has been convincingly disproven, the onset of certain brain-related autoimmune and inflammatory disorders has been found to be temporally associated with the antecedent administration of various vaccines. This study examines whether antecedent vaccinations are associated with increased incidence of obsessive–compulsive disorder (OCD), anorexia nervosa (AN), anxiety disorder, chronic tic disorder, attention deficit hyperactivity disorder, major depressive disorder, and bipolar disorder in a national sample of privately insured children.MethodsUsing claims data, we compared the prior year’s occurrence of vaccinations in children and adolescents aged 6–15 years with the above neuropsychiatric disorders that were newly diagnosed between January 2002 and December 2007, as well as two control conditions, broken bones and open wounds. Subjects were matched with controls according to age, gender, geographical area, and seasonality. Conditional logistic regression models were used to determine the association of prior vaccinations with each condition.ResultsSubjects with newly diagnosed AN were more likely than controls to have had any vaccination in the previous 3 months [hazard ratio (HR) 1.80, 95% confidence interval 1.21–2.68]. Influenza vaccinations during the prior 3, 6, and 12 months were also associated with incident diagnoses of AN, OCD, and an anxiety disorder. Several other associations were also significant with HRs greater than 1.40 (hepatitis A with OCD and AN; hepatitis B with AN; and meningitis with AN and chronic tic disorder).ConclusionThis pilot epidemiologic analysis implies that the onset of some neuropsychiatric disorders may be temporally related to prior vaccinations in a subset of individuals. These findings warrant further investigation, but do not prove a causal role of antecedent infections or vaccinations in the pathoetiology of these conditions. Given the modest magnitude of these findings in contrast to the clear public health benefits of the timely administration of vaccines in preventing mortality and morbidity in childhood infectious diseases, we encourage families to maintain vaccination schedules according to CDC guidelines.

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Immune-metabolic profiling of anorexic patients reveals an anti-oxidant and anti-inflammatory phenotype

Cited by 37 publications

References 34 publications

Prevailing vitamin D status influences mitochondrial and glycolytic bioenergetics in peripheral blood mononuclear cells obtained from adults

Prevailing vitamin D status influences mitochondrial and glycolytic bioenergetics in peripheral blood mononuclear cells obtained from adults

Towards a science of eating disorders: Replacing myths with realities: The fourth Birgit Olsson lecture

Temporal Association of Certain Neuropsychiatric Disorders Following Vaccination of Children and Adolescents: A Pilot Case–Control Study

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