Immune function of plasmacytoid dendritic cells, natural killer cells, and their crosstalk in HBV infection

Golsaz‐Shirazi, Forough; Amiri, Mohammad Mehdi; Shokri, Fazel

doi:10.1002/rmv.2007

Cited by 24 publications

(28 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A large number of studies have confirmed that the immune tolerance of chronic hepatitis B virus infection and chronic hepatitis B patients fail to recover from infection due to HBV specific T cell dysfunction. The severity of liver inflammation and viral clearance is related with the specific cytotoxic T lymphocytes (CTL) 8‐10 . However, the role of many other immune cells and their cytokines in clinical pathogenesis and outcome were not well understood 11 .…”

Section: Discussionmentioning

confidence: 99%

Association of cytokines with hepatitis B virus and its antigen

Chen

et al. 2020

Journal of Medical Virology

View full text Add to dashboard Cite

To investigate the characteristics of cytokines in patients with different HBV infection status and their correlation with HBV DNA, HBsAg, and HBeAg levels. Peripheral blood samples were collected from patients with chronic HBV infection in immune tolerance phase (IT), HBeAg‐positive chronic hepatitis B (CHB), and acute hepatitis B (AHB) groups, and levels of cytokines were detected by Luminex technique, and analyzed by FLEXMAP 3D analyzer. The correlation between cytokines and HBV DNA load, HBsAg, HBeAg, and alanine aminotransferase (ALT) level in patients with chronic HBV infection was analyzed. In total 312 subjects (184 males and 128 females) were enrolled in the study. There were significant differences among IT, CHB, and AHB groups in Flt‐3L value (P = .003; H = 12.312), IFN‐γ (P = .001; H = 11.723), IL‐10 (P = .001; H = 18.736), IL‐17A ((P = .001; H = 12.735), and TGF‐β1 (P = .001; Z = 48.571). IFN‐α2 levels in CHB group were significantly higher than those in IT and AHB groups (15.24 vs 35.78 pg/mL, P = .000; Z = 3.727; 13.88 vs 35.78 pg/mL, P = .024; Z = −2.258. In CHB group, the levels of HBsAg and ALT were positively correlated with the levels of IL‐10 (r = .173; P = .006; r = 0.176; P = .006, respectively), while HBeAg level was positively correlated with the IFN‐α2 level (r = .153; P = .016). In AHB group, the HBsAg level was positively correlated with Flt‐3L, IFN‐α2, IL‐10, and IL‐6 (r = .402; P = .023; r = .436; P = .016; r = .524, P = .002; r = .405; P = .022, respectively). HBeAg level was positively correlated with IFN‐γ and IL‐17A levels (r = .400; P = .023; r = .373; P = .036, respectively), and ALT level was positively correlated with IL‐6 levels (r = .367; P = .039). In either AHB or CHB group, HBV DNA load was only related to TGF‐β level (r = .493; P = .004; r = −.218, P = 0.009 respectively). The correlation between Flt‐3L and HBsAg (F = 7.422; P = .007); IL‐17, IL‐6, and HBeAg (F = 5.757; P = .017; F = 6.156; P = .014) were statistically significant. There was significant correlation between TGF‐β2 and HBV DNA (F = 11.795; P = .001), and between ALT and HBsAg, HBV DNA (F = 26.089; P = .000; F = 4.724; P = .031). HBsAg, HBeAg, and HBV DNA were correlated with cytokines and ALT in patients with HBV infection. The level of IFN‐α2 was significantly higher in patients with CHB. HBV DNA load was only correlated with the level of TGF‐β in acute or CHB.

show abstract

Section: Discussionmentioning

confidence: 99%

Association of cytokines with hepatitis B virus and its antigen

Chen

et al. 2020

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…Even so, increased secretion of IL-10 upon infection has been reported, which could explain the lack of response described for this virus, as this cytokine will induce a state of tolerance in the surveilling immune cells (305,306). Remarkably, since it has been described that pDCs from HBV infected patients are impaired in their capacities to secrete IFN, this also results in a decreased capacity to induce the proper activation of NK cells (302,307).…”

Section: Hepatitis B Virusmentioning

confidence: 99%

The Role of Dendritic Cells During Infections Caused by Highly Prevalent Viruses

et al. 2020

View full text Add to dashboard Cite

Dendritic cells (DCs) are a type of innate immune cells with major relevance in the establishment of an adaptive response, as they are responsible for the activation of lymphocytes. Since their discovery, several reports of their role during infectious diseases have been performed, highlighting their functions and their mechanisms of action. DCs can be categorized into different subsets, and each of these subsets expresses a wide arrange of receptors and molecules that aid them in the clearance of invading pathogens. Interferon (IFN) is a cytokine-a molecule of protein origin-strongly associated with antiviral immune responses. This cytokine is secreted by different cell types and is fundamental in the modulation of both innate and adaptive immune responses against viral infections. Particularly, DCs are one of the most important immune cells that produce IFN, with type I IFNs (α and β) highlighting as the most important, as they are associated with viral clearance. Type I IFN secretion can be induced via different pathways, activated by various components of the virus, such as surface proteins or genetic material. These molecules can trigger the activation of the IFN pathway trough surface receptors, including IFNAR, TLR4, or some intracellular receptors, such as TLR7, TLR9, and TLR3. Here, we discuss various types of dendritic cells found in humans and mice; their contribution to the activation of the antiviral response triggered by the secretion of IFN, through different routes of the induction for this important antiviral cytokine; and as to how DCs are involved in human infections that are considered highly frequent nowadays.

show abstract

“…The roles of NK cells in acute hepatitis B infection have been extensively studied in mice and humans. Early and rapid activation of NK cells has been reported to contribute to HBV clearance, where their dysfunction was associated with the persistence of HBV infection (14). γδT cells are innate immune cells that are not dissimilar to NK cells, which share some common characteristics with regards to virus clearance, including the expression of cytotoxic molecules, including granzyme and perforin, or the production of inflammatory cytokines, including IFN-γ and TNF-α (17).…”

Section: Discussionmentioning

confidence: 99%

“…The innate immune system can respond very rapidly during the early or acute stages of infection to exert functions and boost the subsequent specific immunity. Compared with the extensively studied HBV-specific immunity, mechanisms of innate immune responses during the early stages of HBV infection remain to be defined (14–16).…”

Section: Introductionmentioning

confidence: 99%

Increase in liver γδ�T�cells with concurrent augmentation of IFN‑β production during the early stages of a mouse model of acute experimental hepatitis B virus infection

et al. 2019

View full text Add to dashboard Cite

The role of γδ T cells in acute hepatitis B virus (HBV) infection remains unclear. For the present study, a mouse model of acute HBV infection was constructed using hydrodynamic injection-based transfection of an HBV DNA plasmid (pHBV). Subsequent changes in the percentages of γδ T cells, expression of activation molecules (CD25 and CD69) and the production of the inflammatory cytokines interferon (IFN)-γ and tumor necrosis factor-α (TNF-α) by liver γδ T cells were investigated using fluorescence-activated cell sorting (FACS). Additionally, the immune responses in the mouse liver were evaluated dynamically by measuring cytokine mRNA expression (IFN-α, IFN-β, IFN-γ or TNF-α) using reverse transcription-quantitative PCR, and other populations of immune cells, including CD4+T, CD8+T, natural killer (NK) or natural killer T (NKT) cells, using FACS. On day 1 following acute HBV infection, the percentage of liver γδ T cells was significantly increased along with the high expression of HBV markers. Additionally, liver γδ T cells displayed peak expression of the activation marker CD69 and peak IFN-γ production within this timeframe. IFN-β mRNA expression and the percentage of NK cells were elevated significantly on day 1 in liver tissues. However, there were no significant changes in the spleen or peripheral γδ T cells. Therefore, these data suggested that during the early stages of acute HBV infection, significantly increased numbers of liver γδ T cells may be involved in the enhanced immune response to the increased expression of HBV markers in the liver.

show abstract

Immune function of plasmacytoid dendritic cells, natural killer cells, and their crosstalk in HBV infection

Cited by 24 publications

References 85 publications

Association of cytokines with hepatitis B virus and its antigen

Association of cytokines with hepatitis B virus and its antigen

The Role of Dendritic Cells During Infections Caused by Highly Prevalent Viruses

Increase in liver γδ�T�cells with concurrent augmentation of IFN‑β production during the early stages of a mouse model of acute experimental hepatitis B virus infection

Contact Info

Product

Resources

About