Immobilization ofPseudomonas fluorescenslipase on hydrophobic supports and application in biodiesel synthesis by transesterification of vegetable oils in solvent-free systems

Abstract

This work describes the preparation of biocatalysts for ethanolysis of soybean and babassu oils in solvent-free systems. Polystyrene, Amberlite (XAD-7HP), and octyl-silica were tested as supports for the immobilization of Pseudomonas fluorescens lipase (PFL). The use of octyl-silica resulted in a biocatalyst with high values of hydrolytic activity (650.0 ± 15.5 IU/g), immobilization yield (91.3 ± 0.3 %), and recovered activity (82.1 ± 1.5 %). PFL immobilized on octyl-silica was around 12-fold more stable than soluble PFL, at 45 °C and pH 8.0, in the presence of ethanol at 36 % (v/v). The biocatalyst provided high vegetable oil transesterification yields of around 97.5 % after 24 h of reaction using babassu oil and around 80 % after 48 h of reaction using soybean oil. The PFL-octyl-silica biocatalyst retained around 90 % of its initial activity after five cycles of transesterification of soybean oil. Octyl-silica is a promising support that can be used to immobilize PFL for subsequent application in biodiesel synthesis.

Lipase‐Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids

“…Talukder et al [11] reported that in the methanolysis of free fatty acids catalyzed by Novozym 435 (CALB immobilized on acrylic resin), the highest initial reaction rate was achieved at 60 °C, but that the maximum biodiesel yield at this temperature was slightly lower than at 40 °C. A 1:7 HWCO/ethanol molar ratio was chosen on the basis of the work by Lima et al [30], who studied the enzymatic transesterification of vegetable oils using ethanol as acyl acceptor. Although the stoichiometric molar ratio for the esterification of FFAs is 1:1, excess ethanol was used to displace the esterification equilibrium towards the products.…”

Classification of lipolytic enzymes and their biotechnological applications in the pulping industry

“…Numerous microorganisms are known to produce lipases when incubated with lipid substrates (Haba et al 2000). The majority of bacterial lipases originate from Gram-negative bacteria, the most valuable being Pseudomonas, which includes at least 7 lipaseproducing species, which are P. aeruginosa (Prasad 2014;Tielen et al 2013), Pseudomonas cepacia (Badgujar et al 2016;Cao et al 2016;Sasso et al 2016), Pseudomonas alcaligenes (Chen et al 2014;Patel et al 2014), Pseudomonas glumae (Knapp et al 2016), P. fluorescens (Guldhe et al 2015;Lima et al 2015;Xun et al 2012), Pseudomonas fragi (Dey et al 2014;Santarossa et al 2005), and Pseudomonas putida (Fatima et al 2014). …”

Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases

“…Activities of the biocatalysts CALB-SMMP-octyl and CALB-SMMPoctyl-glu were similar, although recovered activities were different for those biocatalysts. This difference indicates mass transfer delays into the internal surface of the biocatalyst, very common for highloaded biocatalysts [1,2,4,21].…”

“…Most of the enzymes, including lipases for synthesis in organic medium, have their stability and reusability improved by immobilization onto solid supports [1][2][3]. Although several immobilized lipases are commercially available (e.g., Novozym 435, Amano lipase PS, etc.…”