Acta Biotechnol. volume 18, issue 2, P135-146 1998 DOI: 10.1002/abio.370180208 View full text
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E. Miller

Abstract: The formation of reactive groups on polyamide nets (nylon 6) and the subsequent immobilization of glucoamylase were investigated. Different mesh sizes of the nets and two chemical methods of enzyme coupling -i) partial hydrolysis of the polyamide with subsequent glutaraldehyde binding and ii) 0-alkylation of the carrier using a treatment with a benzene-methyl sulphate mixture -were usedThe reactivity of immobilized glucoamylase (GA) was tested by hydrolysis reactions using 1% starch solutions. The highest rea…

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