Immobilization of DNA through Intercalation at Self-Assembled Monolayers on Gold

Abstract

A novel acridine derivative (1) connected with a disulfide bond through a long methylene spacer has been synthesized, and its interactions with DNA in solutions and at a self-assembled monolayer surface on gold have been studied. Spectroscopic and thermal data indicated that 1 intercalated successfully to DNA in buffer solutions. The mixed monolayers of 1 and 2, which has a similar molecular structure to that of 1 except for the lack of the acridine moiety, on gold substrates were then prepared. When DNA binding properties onto these monolayers were examined by means of a quartz crystal microbalance, there was a monolayer composition (mole fraction of 1 in the mixed monolayer, 0.04) at which the most effective binding of DNA took place. The interaction of this DNA-immobilized monolayer with polylysines (d, l) was examined by comparing circular dichroism spectra before and after binding of polylysines, and such a two-dimensionally immobilized DNA was found to have an ability to bind poly-l-lysine predominately.

Raman study on the structure of adlayers formed on silver from mixtures of 2-aminoethanethiol and 3-mercaptopropionic acid

“…1 -4 The metal surfaces modified with adlayers formed from these organosulphur compounds have attracted considerable attention over the past years because of very large technological applications of such systems. The following representative applications of metals modified with organic adlayers may serve to illustrate their wide technological importance: construction of chemical 5,6 and biochemical 7,8 sensors; providing microenvironments on metal surfaces similar to biological membranes that allows adsorption of proteins without denaturization, 7,9 immobilization of DNA 10,11 or adhesion of living cells to the metal surface; 7,12 preparation of surfaces that resist the adsorption of proteins and cell attachment; 12,13 preparation of surfaces for controlled growth of crystals; 14 micro contact printing of metals; 15,16 protection of metals against corrosion; 17,18 antiadherent coatings of metal surfaces. 19 To prepare the metal substrates (as electrodes) for some applications, the surface of the metal is modified with the organic adlayer formed from substituted alkanethiols (typically ω-substituted: HS-(CH 2 n -X).…”

Deposition of complement protein C3b on mixed self‐assembled monolayers carrying surface hydroxyl and methyl groups studied by surface plasmon resonance

“…Self-assembled monolayers (SAMs) made of alkanethiols with a long methylene chain give well defined model surfaces for studies of interfacial phenomena and intermolecular interactions. [8][9][10][11][12] It is generally accepted that the alternative pathway of complement is initiated during contact of blood with artificial materials. The primary event is the interaction of the C3 molecule with the surface.…”

Study on the interaction between DNA and protein induced by anticancer drug carboplatin

“…There were many methods to immobilize DNA on the surface of the electrode, such as chemisorption [23], molecule self-assembling [24], and covalent bond combination [25]. Here the method of the covalent bond combination was adopted, i.e., DNA was immobilized on the cysteamine-modified PQC surface by EDC.…”