Immobilization of cellulase in nanofibrous PVA membranes by electrospinning

Wu, Lili; Yuan, Xiaoyan; Sheng, Jing

doi:10.1016/j.memsci.2004.10.024

Cited by 299 publications

(207 citation statements)

References 18 publications

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“…These values are higher than those obtained by Wu et al [34], who recorded only 36% residual activity after 6 reuses.…”

Section: Discussioncontrasting

confidence: 73%

Immobilization of Commercial Cellulase and Xylanase by Different Methods Using Two Polymeric Supports

Romo-Sánchez¹,

Camacho²,

Ramírez³

et al. 2014

ABB

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Industrial applications require enzymes highly stable and economically viable in terms of reusability. Enzyme immobilization is an exciting alternative to improve the stability of enzymatic processes. The immobilization of two commercial enzymes is reported here (cellulase and xylanase) using three chemical methods (adsorption, reticulation, and crosslinking-adsorption) and two polymeric supports (alginate-chitin and chitosan-chitin). The optimal pH for binding was 4.5 for cellulase and 5.0 for xylanase, and the optimal enzyme concentrations were 170 µg/mL and 127.5 µg/mL respectively, being the chitosan and the ideal support. In some cases, a low concentration of crosslinking agent (glutaraldehyde) improved stability of the immobilization process. Biotechnological characterization showed that the reusability of enzymes was the most striking finding, particularly of immobilized cellulase using glutaraldehyde, which after 19 cycles retained 64% activity. These results confirm the economic and biotechnical advantages of enzyme immobilization for a range of industrial applications.

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“…These values are higher than those obtained by Wu et al [34], who recorded only 36% residual activity after 6 reuses.…”

Section: Discussioncontrasting

confidence: 73%

Immobilization of Commercial Cellulase and Xylanase by Different Methods Using Two Polymeric Supports

Romo-Sánchez¹,

Camacho²,

Ramírez³

et al. 2014

ABB

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“…2 PVA is widely used in textile, adhesive, cosmetic, food, drug, paper, and packaging industries. The main functional uses of PVA include filtration, catalysis, 5,6 membranes, optics, drug release, 7,8 enzyme mobilization, 8 tissue engineering, 9 among others. 10 Many of the key properties of PVA, such as those mentioned above, are the result of its significant cohesive energy due to its polarity from hydroxyl groups.…”

Section: Introductionmentioning

confidence: 99%

Effect of Moisture on Electrospun Nanofiber Composites of Poly(vinyl alcohol) and Cellulose Nanocrystals

et al. 2010

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. 2010. Effect of moisture on electrospun nanofiber composites of poly(vinyl alcohol) and cellulose nanocrystals. Biomacromolecules, volume 11, number 9, pages 2471-2477. © 2010 American Chemical Society (ACS)Reprinted with permission from American Chemical Society. Effect of Moisture on Electrospun Nanofiber Composites ofPoly(vinyl alcohol) and Cellulose Nanocrystals The effect of humidity on the morphological and thermomechanical properties of electrospun poly(vinyl alcohol) (PVA) fiber mats reinforced with cellulose nanocrystals (CNs) was investigated. Scanning electron microscopy (SEM) images revealed that the incorporation of CNs improved the morphological stability of the composite fibers even in high humidity environments. Thermal and mechanical properties of the electrospun fiber mats were studied by using differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), and large deformation tensile tests under controlled humidity and temperatures. The balance between the moisture-induced plasticization and the reinforcing effect of rigid CN particles was critical in determining the thermomechanical behaviors of the electrospun fiber mats. Results indicated that the stabilizing effect of the CNs in the PVA matrix might be compromised by water absorption, disrupting the hydrogen bonding within the structure. The amount of this disruption depended on the surrounding humidity and the CN loading. The reduction in tensile strength of neat PVA fiber mats as they were conditioned from low relative humidity (10% RH) to high relative humidity (70% RH) was found to be about 80%, from 1.5 to 0.4 MPa. When the structure was reinforced with CNs, the reduction in strength was limited to 40%, from 2 to 0.8 MPa over the same range in relative humidity. More importantly, the CN-loaded PVA fiber mats showed a reversible recovery in mechanical strength after cycling the relative humidity. Finally, humidity treatments of the composite PVA fiber mats induced significant enhancement of their strength as a result of the adhesion between the continuous matrix and the CNs.

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“…A large number of synthetic polymers have been successfully electrospun viz. poly-L-lactide (PLLA) (5), polycaprolactone (PCL) (6), poly(lactic-co-glycolic acid) (PLGA) (7), polyvinyl alcohol (PVA) (8) and polyethylene glycol (PEG) (9) etc. However, the lack of cellular recognition sites on synthetic polymers limits their application to tissue engineering.…”

Section: Introductionmentioning

confidence: 99%