Immobilization of cellulase enzyme onto magnetic nanoparticles: Applications and recent advances

Khoshnevisan, Kamyar; Vakhshiteh, Faezeh; Barkhi, Mohammad; Baharifar, Hadi; Poorakbar, Elahe; Zari, Naeemeh; Stamatis, Haralambos; Bordbar, Abdol‐Khalegh

doi:10.1016/j.mcat.2017.09.006

Cited by 103 publications

(42 citation statements)

References 89 publications

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“…Cellulase from Trichoderma sp. has been successfully immobilized on different magnetic nanoparticles, 30 chitosan microparticles, 31 surface modified coffee, 32 silica particles grafted with polyacrylic acid polymer brushes, 33 and as a crosslinked cellulase aggregates, 34 but until now, no immobilization on biochar has been reported. Therefore, cellulase from Trichoderma sp.…”

Section: Introductionmentioning

confidence: 99%

Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass

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Pavlovič

et al. 2019

ACSi

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For improving enzyme utilization in biotechnological processes, process costs have to be reduced, enzyme stability during industrial processes should be enhanced, and the recycle and reuse step should be favorable. The immobilization of enzymes is an important step for enhancing enzyme catalytic properties and operational stability. In order to reduce the costs of immobilization and consequently the cost of processes, a cheaper carrier (e.g. materials reclaimed as by-products) should be used. To achieve this, cellulase from Trichoderma sp. was immobilized on biochar obtained by low temperature hydrothermal carbonization (LTHTC) in two ways: by adsorption and by covalent binding via a crosslinking agent. The effect of immobilization time, enzyme concentration, type and concentration of the crosslinking agent and the types of carrier-biochar (LTHTC of waste from olive oil production (LTHTC of OL waste) or LTHTC of cellulose) on the immobilization efficiency and the residual activity of biocatalyst was studied. Higher immobilization efficiency and residual enzyme activity was achieved when the enzyme was covalently bound to biochar obtained by LTHTC of cellulose.

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Section: Introductionmentioning

confidence: 99%

Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass

Primožič

Podrepšek

Pavlovič

et al. 2019

ACSi

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“…Lately, magnetic nanoparticles have been introduced to the formation of CLEAs to enhance the catalytic properties of the immobilized biocatalysts. The cellulase enzyme has been reported to form a CLEA-MNP composite with increased hydrolytic activity and the potential to stand in extreme environments [33]. The easy separation of MNPs from media solutions and stability of the synthesized complex during frequent use were proposed as key advantages for the promotion of cellulase industrial applications.…”

Section: Enzyme Immobilization On Magnetic Particlesmentioning

confidence: 99%

Magnetic Microreactors with Immobilized Enzymes—From Assemblage to Contemporary Applications

2018

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Microfluidics, as the technology for continuous flow processing in microscale, is being increasingly elaborated on in enzyme biotechnology and biocatalysis. Enzymatic microreactors are a precious tool for the investigation of catalytic properties and optimization of reaction parameters in a thriving and high-yielding way. The utilization of magnetic forces in the overall microfluidic system has reinforced enzymatic processes, paving the way for novel applications in a variety of research fields. In this review, we hold a discussion on how different magnetic particles combined with the appropriate biocatalyst under the proper system configuration may constitute a powerful microsystem and provide a highly explorable scope.

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“…Other nanomaterials like magnetic nanoparticles, graphene oxide nanosheets, nanoclays, etc., have been extensively used as effective techniques to immobilize and stabilize cellulolytic enzymes. Magnetic nanoparticles and graphene nanosheets have been found to impart not only operational and thermal stability but also provide stability against ionic liquids . In addition, use of magnetic nanoparticles facilitates easy recycling of enzymes from the complex hydrolysis mixtures.…”

Section: Stabilization Of Cellulolytic Enzymesmentioning

confidence: 99%

Stable Cellulolytic Enzymes and Their Application in Hydrolysis of Lignocellulosic Biomass

2018

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The effective utilization of lignocellulosic biomass for production of valueadded products in bio-refineries mainly depends on their hydrolysis into constituent soluble sugars. Despite the commercial availability of lignocellulolytic enzymes, there are certain challenges in using them at an industrial scale which arise from their inherent characteristics. Hence, development of industrially potent and stable cellulolytic enzymes is of great importance. While a lot of emphasis has been given to produce the enzymes with high titre and productivity, the properties of these enzymes, which affect their performance in hydrolyzing lignocellulosic residues, have largely been ignored. The various properties that affect the hydrolysis are thermal stability, catalytic efficiency, non-specific adsorption, end-product inhibition resistance, and shear inactivation. This review discusses several approaches such as finding new enzymes from extremophiles, recombinant production of cellulolytic enzymes in industrial strains, directed evolution and mutagenesis, and formation of the enzyme aggregates that are being investigated to develop stable cellulase enzymes. The authors also suggest that for a meaningful information on the modified cellulolytic enzymes and their application in cellulose hydrolysis, they should be evaluated under practical process condition.

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Immobilization of cellulase enzyme onto magnetic nanoparticles: Applications and recent advances

Cited by 103 publications

References 89 publications

Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass

Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass

Magnetic Microreactors with Immobilized Enzymes—From Assemblage to Contemporary Applications

Stable Cellulolytic Enzymes and Their Application in Hydrolysis of Lignocellulosic Biomass

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