Immobilization of Candida antarctica Lipase B by Adsorption to Green Coconut Fiber

Use of Yarrowia lipolytica Lipase Immobilized in Cell Debris for the Production of Lipolyzed Milk Fat (LMF)

“…Reuse of C. antarctica lipase B (CALB) immobilized by physical adsorption on coconut fiber in comparison to CALB immobilized on acrylic resin (commercial lipase—Novozymes 435) showed that the first retained less than 50% of its initial hydrolytic activity after the third cycle, whereas Novozyme 435 could be used with more than 70% of initial activity for ten cycles [29]. The desorption of the enzyme during reaction because of the weak electrostatic interactions between the enzyme and coconut fiber support could be the reason why CALB immobilized on coconut fiber presented lower residual activity.…”

“…In the present work it is believed that lipase is strongly bound to cell debris. Therefore, a washing step was introduced between cycles to remove any retaining substrate or product, as described by Brigida et al [29]. The washing procedure with Tris-HCl buffer (pH 7.5) was worse for biocatalyst reuse than without any washing procedure (Figure 4).…”

Waste Management in the Agri-Food Industry: The Conversion of Eggshells, Spent Coffee Grounds, and Brown Onion Skins into Carriers for Lipase Immobilization

“…Furthermore, to commercially available forms of pure cellulose and its modified derivatives, water-insoluble lignocellulosic material from agri-food industry waste can also be used as an even more cost-effective carrier for lipase immobilization. This can be demonstrated by research on immobilization of lipases on olive pomace [116][117][118], rice husks [53,57], corn stalks [119], coconut shells or fibers [119,120], palm stalks [119], and loofah sponges [121]. Immobilization of lipase was achieved by adsorption to pretreated and processed waste or by covalent binding to the chemically modified carrier (e.g., chemically modified pretreated waste material).…”

“…T. lanuginosus lipase immobilized on olive pomace [116][117][118] showed operational stability in a functional test reaction (synthesis of biodiesel from pomace oil with methanol) in up to 10 consecutive batches. Corici et al [53] and Brigida et al [119] immobilized lipase by adsorption on rice husks and coconut fibers, respectively, while Ittrat et al [120] used corn stalks, palm stalks, coconut shells, corn cobs, rice husks, Wodyetia bifurcata AK Irvine leaves, and Salacca wallichiana stems. The worst results were reported by Corici et al [53], probably due to the low activity of immobilized lipase and low operational stability.…”

Determinação Do Fator De Efetividade Para Enzimas Imobilizadas Usando Métodos De Regressão Simbólica via Programação Genética

“…As lipases, por exemplo, possui um sítio ativo coberto por uma região flexível chamada de tampa ou "lid" e a interação com uma superfície hidrofóbica do suporte durante a imobilização desloca a tampa para uma conformação aberta que torna o sítio ativo mais acessível pelo substrato e assim provoca o aumento da atividade catalítica do biocatalisador imobilizado (KALANTARI et al, 2017). Para o uso de resíduos agrícolas ou agroindustriais nos processos de imobilização, tem-se utilizado a imobilização multipontual de lipases, lacases e leveduras conforme descrito na literatura (BRÍGIDA et al, 2008;MENDES et al, 2013;ALMEIDA et al, 2017). E também outros métodos de imobilização como a adsorção física de lacase, β-fructofuranosidase para diversas aplicações (MUSSATO et al, 2009).…”