Immobilizable fluorescent probes for monitoring the mitochondria microenvironment: a next step from the classic

Abstract

Immobilizable probes represent a valuable trend.

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

“…Fortunately, fluorescence imaging is emerging as a robust technique for direct visualization of cell events owing to its outstanding sensitivity, high temporal‐spatial resolution, and noninvasive operation [7–10] . Numerous fluorescent probes for various bioactive species and microenvironments have been reported and utilized to explore physiological and pathological processes [11–23] . Particularly, owing to the easy synthesis and modification as well as excellent repeatability, small molecular fluorescent probes are widely employed [24–27] .…”

Multifunctional self-assembled peptide nanoparticles for multimodal imaging-guided enhanced theranostic applications against glioblastoma multiforme

“…uorescence intensity in the nuclear periphery region. 42,43 The merged CLSM images with blue colored holes show a stained nuclear region (Fig. 2c and d) while blank holes showed an unstained nuclear region (Fig.…”

Hybridization of tumor homing and mitochondria-targeting peptide domains to design novel dual-imaging self-assembled peptide nanoparticles for theranostic applications

“…This ultimately produces reactive oxygen species (ROS) that causes mtDNA damage, whilst, it releases cytochrome c to promotes the formation of apoptosome, causing activation of Caspase-3 enzyme as showed in Figure 2h [41]. The bright green uorescence is due to activated Caspase-3 which is well-overlapped with red uorescence as presented in the merged image to give bright yellow uorescence intensity in nuclear periphery region [42,43]. Additionally, we also performed a Bio-TEM imaging study using Cy5.5@SAPD nanoparticles treated U87MG cancer cells, the TEM images showed pinocytic internalization of nanoparticles as shown in Figure 2i.…”