Imaging the oxygen wave with a single bioluminescent bacterium

Abstract

We developed a capability of a monolayer of bioluminescent (BL) bacteria for spatiotemporally visualizing the heterogeneous distribution and dynamic evolution of interfacial oxygen concentration, resulting in the discovery of spontaneous...

Optical mapping of the evolution of water content during the swelling of hydrophilic polymers

“…3d. The diffusion coefficient (D) of water molecules within this hydrogel was finally calculated to be 6 Â 10 À12 m 2 s À1 according to the linear diffusion model (Length = Sqrt(2Dt)), 30 which was consistent with previous literature reports. 17 In summary, we have developed a new optical methodology to quantitatively image the local water content of hydrogels as a typical hydrophilic polymer by advanced total internal reflection optical microscopy.…”

“…3d. The diffusion coefficient (D) of water molecules within this hydrogel was finally calculated to be 6 × 10 −12 m 2 s −1 according to the linear diffusion model (Length = Sqrt(2D t )), 30 which was consistent with previous literature reports. 17…”

Intermittent proton bursts of single lactic acid bacteria

“…The proton burst event was random and independent among different individuals; this phenomenon was only accessible at the single bacterium level, underscoring the value of the present methodology for studying single bacteria dynamics. 45–48…”

“…The proton burst event was random and independent among different individuals; this phenomenon was only accessible at the single bacterium level, underscoring the value of the present methodology for studying single bacteria dynamics. [45][46][47][48] Although the proton bursts and membrane potential dynamics are phenomenologically and mechanistically correlated with each other, the present pH e recording exhibited several important strengths for studying membrane potential dynamics over conventional voltage-sensitive dyes such as TMRM. First, while conventional organic and genetic staining required molecular modication to the bacterium and thus inevitably disturbed its physiological states, the present approach minimized such disturbance because the pH e indicator dyes were distributed in the extracellular medium.…”

Implication of storage conditions on luminescence response from Photobacterium phosphoreum in free and immobilized form

“…The immediate concern is the marked variability in luminescence emission during experimental analysis [9]. The dynamic nature of the bacteria in suspension and thereof sensitivity toward molecular oxygen [10], temperature, and the ambient environment are the presumptions for such behaviors [11]. Another difficulty associated with the application of free BB is their viability over an extended duration.…”