Imaging striatal dopamine release using a nongenetically encoded near infrared fluorescent catecholamine nanosensor

Beyene, Abraham G.; Delevich, Kristen; Bonis-O’Donnell, Jackson Travis Del; Piekarski, David J.; Lin, Wan Chen; Thomas, A. Wren; Yang, Sarah J.; Kosillo, Polina; Yang, Darwin; Prounis, George S.; Wilbrecht, Linda; Landry, Markita P.

doi:10.1126/sciadv.aaw3108

Cited by 140 publications

(217 citation statements)

References 60 publications

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“…Moreover, SWCNTs functionalized with DNA sequences containing an endonuclease recognition site have been successfully used to study restriction enzyme activity by monitoring their fluorescent emissions [106]. The DNA-SWCNTs have shown increased fluorescence intensity in response to neurotransmitters and have successfully detected dopamine efflux in neuroprogenitor cell cultures [107][108][109][110][111] and in acute brain slices [112,113]. Further, (GT) 6 -SWCNT has successfully detected dopamine and norepinephrine in a broad range of pH and salt concentrations, suggesting the potential compatibility for in-vivo neurophysiological use [113,114].…”

Section: Swcnts As Optical Sensorsmentioning

confidence: 99%

Fluorescent Single-Walled Carbon Nanotubes for Protein Detection

Hendler‐Neumark

Bisker

2019

Sensors

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Nanosensors have a central role in recent approaches to molecular recognition in applications like imaging, drug delivery systems, and phototherapy. Fluorescent nanoparticles are particularly attractive for such tasks owing to their emission signal that can serve as optical reporter for location or environmental properties. Single-walled carbon nanotubes (SWCNTs) fluoresce in the near-infrared part of the spectrum, where biological samples are relatively transparent, and they do not photobleach or blink. These unique optical properties and their biocompatibility make SWCNTs attractive for a variety of biomedical applications. Here, we review recent advancements in protein recognition using SWCNTs functionalized with either natural recognition moieties or synthetic heteropolymers. We emphasize the benefits of the versatile applicability of the SWCNT sensors in different systems ranging from single-molecule level to in-vivo sensing in whole animal models. Finally, we discuss challenges, opportunities, and future perspectives.

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Section: Swcnts As Optical Sensorsmentioning

confidence: 99%

Fluorescent Single-Walled Carbon Nanotubes for Protein Detection

Hendler‐Neumark

Bisker

2019

Sensors

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“…Interaction between the SWCNT surface and target analyte is mediated by the coating, which leads to the perturbation of the electronic characteristics of the SWCNT resulting in a measurable fluorescence change. While SWCNT‐based nanosensors generated with CoPhMoRe have shown recent success for imaging analytes in vivo [ 6,20,21 ] and for ex vivo imaging neuromodulation in acute brain slices, [ 22 ] their use has involved undirected biodistribution of SWCNTs in the tissue under investigation. For the purposes of tissue‐specific or targeted sensing, inclusion of targeting moieties such as aptamers or proteins can be achieved via direct covalent attachment to SWCNT surfaces.…”

Section: Introductionmentioning

confidence: 99%

Covalent Surface Modification Effects on Single‐Walled Carbon Nanotubes for Targeted Sensing and Optical Imaging

Chio

Pinals

Murali

et al. 2020

Adv Funct Materials

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Optical nanoscale technologies often implement covalent or noncovalent strategies for the modification of nanoparticles, whereby both functionalizations are leveraged for multimodal applications but can affect the intrinsic fluorescence of nanoparticles. Specifically, single-walled carbon nanotubes (SWCNTs) can enable real-time imaging and cellular delivery; however, the introduction of covalent SWCNT sidewall functionalizations often attenuates SWCNT fluorescence. Recent advances in SWCNT covalent functionalization chemistries preserve the SWCNT's pristine graphitic lattice and intrinsic fluorescence, and here, such covalently functionalized SWCNTs maintain intrinsic fluorescencebased molecular recognition of neurotransmitter and protein analytes. The covalently modified SWCNT nanosensor preserves its fluorescence response towards its analyte for certain nanosensors, presumably dependent on the intermolecular interactions between SWCNTs or the steric hindrance introduced by the covalent functionalization that hinders noncovalent interactions with the SWCNT surface. These SWCNT nanosensors are further functionalized via their covalent handles with a targeting ligand, biotin, to self-assemble on passivated microscopy slides, and these dual-functionalized SWCNT materials are explored for future use in multiplexed sensing and imaging applications.

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“…Single wall carbon nanotubes (SWNTs) comprise a key component of many new nanotechnology applications for sensing, biological imaging, electronics, and gene delivery, among others [1][2][3][4][5][6][7][8][9][10][11] . Noncovalent polymer adsorption is a widely used method to confer and optimize desired functionalities to SWNTs, while solubilizing them in aqueous environments.…”

Section: Introductionmentioning

confidence: 99%

Binding affinity and conformational preferences influence kinetic stability of short oligonucleotides on carbon nanotubes

Alizadehmojarad

Zhou²,

Beyene³

et al. 2020

Preprint

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DNA-wrapped single walled carbon nanotubes (SWNTs) have found a widespread use in a variety of nanotechnology applications. Yet, the relationship between structural conformation, binding affinity and kinetic stability of these polymers on SWNTs remains poorly understood. Here, we used molecular dynamics (MD) simulations and experiments to explore this relationship for short oligonucleotides adsorbed on SWNTs. First, using classical MD simulations of oligonucleotide-(9,4)-SWNT hybrid complexes, we explored the relationship between ssDNA and ssRNA surface conformation and sequence chemistry. We screened the conformation of 36 sequences of short ssDNA and ssRNA polymers on (9,4) SWNT, where the contour lengths were selected so the polymers can, to a first approximation, wrap once around the SWNT circumference. From these screens, we identified structural motifs that we broadly classified into "rings" and "non-rings." Then, several sequences were selected for detailed investigations. We used temperature replica exchange MD calculations to compute two-dimensional free energy landscapes characterizing the conformations of select sequences. "Ring" conformations seemed to be driven primarily by sequence chemistry. Specifically, strong (n,n+2) nucleotide interactions and the ability of the polymer to form compact structures, as for example, through sharp bends in the nucleotide backbone, correlated with ring-forming propensity. However, ring-formation probability was found to be uncorrelated with free energy of oligonucleotide binding to SWNTs (∆Gbind). Conformational analyses of oligonucleotides, computed free energy of binding of oligonucleotides to SWNTs, and experimentally determined kinetic stability measurements show that ∆Gbind is the primary correlate for kinetic stability. The probability of the sequence to adopt a compact, ring-like conformation is shown to play a secondary role that still contributes measurably to kinetic stability. For example, sequences that form stable compact rings (C-rich sequences) could compensate for their relatively lower ∆Gbind and exhibit kinetic stability, while sequences with strong ∆Gbind (such as (TG)3(GT)3) were found to be kinetically stable despite their low ring formation propensity. We conclude that the stability of adsorbed oligonucleotides is primarily driven by its free energy of binding and that if ring-like structural motifs form, they would contribute positively to stability.

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Imaging striatal dopamine release using a nongenetically encoded near infrared fluorescent catecholamine nanosensor

Cited by 140 publications

References 60 publications

Fluorescent Single-Walled Carbon Nanotubes for Protein Detection

Fluorescent Single-Walled Carbon Nanotubes for Protein Detection

Covalent Surface Modification Effects on Single‐Walled Carbon Nanotubes for Targeted Sensing and Optical Imaging

Binding affinity and conformational preferences influence kinetic stability of short oligonucleotides on carbon nanotubes

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