Imaging Stem Cells Implanted in Infarcted Myocardium

Zhou, Rong; Acton, Paul D.; Ferrari, Victor A.

doi:10.1016/j.jacc.2006.08.026

Cited by 127 publications

(159 citation statements)

References 150 publications

(118 reference statements)

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“…These parameters, however, cannot be readily obtained in the clinical setting in contrast to preclinical animal studies since extensive histologic analysis of myocardial tissue after cell transplantation cannot be performed in patients. Therefore, the development of noninvasive imaging approaches to monitor the fate of implanted stem cells is needed and may help in successful translation of stem cell therapies to patients (18)(19)(20)(21)(22). Several different imaging approaches for visualizing stem cell fate in the heart are currently under investigation, including magnetic resonance (MR) imaging, optical imaging, and PET.…”

Section: Molecular Imaging: Imaging Gene Expression In Human Mesenchymentioning

confidence: 99%

“…Furthermore, conflicting reports on the clinical benefit in patients with coronary artery disease following cell transplantation have raised several questions, including which subpopulation of patients with coronary artery disease may benefit most from which cell type implanted through which delivery approach in the heart (14)(15)(16)(17). The development of objective and quantitative noninvasive (imaging) approaches for tracking stem cell fate, including stem cell location, survival, engraftment, and differentiation, may help in better understanding stem cell biology and may lead to better patient stratification and eventual successful implementation of cardiac stem cell therapy into the clinic (18)(19)(20)(21)(22).…”

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confidence: 99%

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Imaging Gene Expression in Human Mesenchymal Stem Cells: From Small to Large Animals

Willmann¹,

Paulmurugan²,

Rodriguez-Porcel³

et al. 2009

Radiology

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Purpose:To evaluate the feasibility of reporter gene imaging in implanted human mesenchymal stem cells (MSCs) in porcine myocardium by using clinical positron emission tomography (PET)-computed tomography (CT) scanning. Materials and Methods:Animal protocols were approved by the Institutional Administrative Panel on Laboratory Animal Care. Transduction of human MSCs by using different doses of adenovirus that contained a cytomegalovirus (CMV) promoter driving the mutant herpes simplex virus type 1 thymidine kinase reporter gene (Ad-CMV-HSV1-sr39tk) was characterized in a cell culture. A total of 2.25 ϫ 10 6 transduced (n ϭ 5) and control nontransduced (n ϭ 5) human MSCs were injected into the myocardium of 10 rats, and reporter gene expression in human MSCs was visualized with micro-PET by using the radiotracer 9-(4-[fluorine 18]-fluoro-3-hydroxymethylbutyl)-guanine (FHBG). Different numbers of transduced human MSCs suspended in either phosphatebuffered saline (PBS) (n ϭ 4) or matrigel (n ϭ 5) were injected into the myocardium of nine swine, and gene expression was visualized with a clinical PET-CT. For analysis of cell culture experiments, linear regression analyses combined with a t test were performed. To test differences in radiotracer uptake between injected and remote myocardium in both rats and swine, one-sided paired Wilcoxon tests were performed. In swine experiments, a linear regression of radiotracer uptake ratio on the number of injected transduced human MSCs was performed.

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Section: Molecular Imaging: Imaging Gene Expression In Human Mesenchymentioning

confidence: 99%

mentioning

confidence: 99%

Imaging Gene Expression in Human Mesenchymal Stem Cells: From Small to Large Animals

Willmann¹,

Paulmurugan²,

Rodriguez-Porcel³

et al. 2009

Radiology

View full text Add to dashboard Cite

show abstract

“…Positron emission tomography (PET) is a functional imaging modality that has proven to be an essential tool for in vivo molecular imaging, due to its high sensitivity, allowing for picomolar levels of detection [1]. PET is often used for diagnoses of cancer using the clinically approved radiopharmaceutical [ 18 F] fluoro-2-deoxy-Dglucose (FDG), a glucose analog that remains trapped in the cell during glycolysis [2].…”

Section: Introductionmentioning

confidence: 99%

“…Magnetic resonance imaging (MRI) offers high spatial resolution and unparalleled soft tissue contrast [6], which is important for accurate VOI analysis. Furthermore, Superparamagnetic Iron Oxide (SPIO) particles can be used to label cells for MRI cell tracking studies [1].…”

Section: Introductionmentioning

confidence: 99%

New Software for Multimodal Image Registration with Application in Cell Tracking Studies

E¹

2014

J Stem Cell Res Ther

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“…Recently, the rapid progress of molecular imaging techniques has enabled noninvasive evaluation of the characteristics of transplanted stem cells and, in particular, of their viability. Molecular imaging allows real-time visualization of survival and quantitative viability measurements of stem cells implanted into an injured site by using stem cells labeled with proper radiotracers or reporter-based gene expression schemes [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Optical Imaging for Stem Cell Differentiation to Neuronal Lineage

Hwang

Lee

2012

Nucl Med Mol Imaging

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In regenerative medicine, the prospect of stem cell therapy holds great promise for the recovery of injured tissues and effective treatment of intractable diseases. Tracking stem cell fate provides critical information to understand and evaluate the success of stem cell therapy. The recent emergence of in vivo noninvasive molecular imaging has enabled assessment of the behavior of grafted stem cells in living subjects. In this review, we provide an overview of current optical imaging strategies based on cell-or tissuespecific reporter gene expression and of in vivo methods to monitor stem cell differentiation into neuronal lineages. These methods use optical reporters either regulated by neuron-specific promoters or containing neuron-specific microRNA binding sites. Both systems revealed dramatic changes in optical reporter imaging signals in cells differentiating into a neuronal lineage. The detection limit of weak promoters or reporter genes can be greatly enhanced by adopting a yeast GAL4 amplification system or an engineering-enhanced luciferase reporter gene. Furthermore, we propose an advanced imaging system to monitor neuronal differentiation during neurogenesis that uses in vivo multiplexed imaging techniques capable of detecting several targets simultaneously.

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Imaging Stem Cells Implanted in Infarcted Myocardium

Cited by 127 publications

References 150 publications

Imaging Gene Expression in Human Mesenchymal Stem Cells: From Small to Large Animals

Imaging Gene Expression in Human Mesenchymal Stem Cells: From Small to Large Animals

New Software for Multimodal Image Registration with Application in Cell Tracking Studies

Optical Imaging for Stem Cell Differentiation to Neuronal Lineage

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