Imaging phosphatidylinositol 4‐phosphate dynamics in living plant cells

Vermeer, Joop E. M.; Thole, Julie M.; Goedhart, Joachim; Nielsen, Erik; Munnik, Teun; Gadella, Theodorus W. J.

doi:10.1111/j.1365-313x.2008.03679.x

Cited by 158 publications

(192 citation statements)

References 86 publications

Supporting

Mentioning

180

Contrasting

Order By: Relevance

“…It is intriguing that the exocyst complexes move back and forth between the plasma membrane and the cytoplasm (Fendrych et al, 2010(Fendrych et al, , 2013. In addition, FM4-64 is observed at the cell plate as early as the IPA stage, and phosphatidylinositol-4-phosphate (originating from both the Golgi and the plasma membrane) is integrated into the growing cell plate (Vermeer et al, 2009), with the accumulation of YFP-2x-FYVE-positive endosomes at the periphery of the growing cell plate (Vermeer et al, 2006(Vermeer et al, , 2009.…”

Section: Discussionmentioning

confidence: 99%

Vesicle Dynamics during Plant Cell Cytokinesis Reveals Distinct Developmental Phases

et al. 2017

View full text Add to dashboard Cite

Cell division in plant cells requires the deposition of a new cell wall between the two daughter cells. The assembly of this plate requires the coordinated movement of cargo vesicles whose size is below the diffraction-limited resolution of the optical microscope. We combined high spatial and temporal resolution confocal laser scanning microscopy with advanced imageprocessing tools and fluorescence fluctuation methods and distinguished three distinct phases during cell plate expansion in tobacco (Nicotiana tabacum) 'Bright Yellow-2' cells: massive delivery of preexisting vesicles to a disk-shaped region at the equatorial plane precedes a primary rapid expansion phase followed by a secondary, slow expansion phase during which the extremity of the circular plate seeks contact with the mother wall and brings about the separation of the two portions of cytoplasm. Different effects of pharmacological inhibition emphasize the distinct nature of the assembly and expansion mechanisms characterizing these phases.

show abstract

Section: Discussionmentioning

confidence: 99%

Vesicle Dynamics during Plant Cell Cytokinesis Reveals Distinct Developmental Phases

et al. 2017

View full text Add to dashboard Cite

show abstract

“…of lipids bound in vitro. Interestingly, these lipids are enriched in membrane rafts from plants (Vermeer et al, 2009;Furt et al, 2010) or animal cells (Hope and Pike, 1996;Pike et al, 2002), suggesting that RemCA lipidspecificity contributes to driving the segregation of StREM1.3 into membrane microdomains.…”

Section: Remca Directly Binds To Lipid Bilayers In Vitromentioning

confidence: 99%

“…Indeed, polyphosphoinositides and sphingolipids are enriched in membrane rafts from plants (Vermeer et al, 2009;Furt et al, 2010) and animal cells (Hope and Pike, 1996), the latter being also enriched in phosphatidylserine (PS) (Pike et al, 2002). The ability to specifically target DIMs, therefore, might be related to the differential affinity of RemCA to certain negative lipids, as known for SNARE proteins (Mima and Wickner, 2009).…”

Section: Toward Understanding the Molecular Bases Of Specific Associamentioning

confidence: 99%

Plasma Membrane Localization of Solanum tuberosum Remorin from Group 1, Homolog 3 Is Mediated by Conformational Changes in a Novel C-Terminal Anchor and Required for the Restriction of Potato Virus X Movement

et al. 2012

View full text Add to dashboard Cite

The formation of plasma membrane (PM) microdomains plays a crucial role in the regulation of membrane signaling and trafficking. Remorins are a plant-specific family of proteins organized in six phylogenetic groups, and Remorins of group 1 are among the few plant proteins known to specifically associate with membrane rafts. As such, they are valuable to understand the molecular bases for PM lateral organization in plants. However, little is known about the structural determinants underlying the specific association of group 1 Remorins with membrane rafts. We used a structure-function approach to identify a short C-terminal anchor (RemCA) indispensable and sufficient for tight direct binding of potato (Solanum tuberosum) REMORIN 1.3 (StREM1.3) to the PM. RemCA switches from unordered to a-helical structure in a nonpolar environment. Protein structure modeling indicates that RemCA folds into a tight hairpin of amphipathic helices. Consistently, mutations reducing RemCA amphipathy abolished StREM1.3 PM localization. Furthermore, RemCA directly binds to biological membranes in vitro, shows higher affinity for Detergent-Insoluble Membranes lipids, and targets yellow fluorescent protein to Detergent-Insoluble Membranes in vivo. Mutations in RemCA resulting in cytoplasmic StREM1.3 localization abolish StREM1.3 function in restricting potato virus X movement. The mechanisms described here provide new insights on the control and function of lateral segregation of plant PM.

show abstract

“…The phosphatidylinositol (PI) monophosphates [PI(3)P and PI(4)P specifically] and PS, on the other hand, are excellent candidates for interacting with POL and PLL1 in planta because these lipids are present, to varying degrees, in the inner leaf of plasma membrane (Uemura et al, 1995;Vance and Steenbergen, 2005;Skwarek and Boulianne, 2009;Vermeer et al, 2009). For a few of these lipids, in-depth studies have been performed to visualize how the lipids behave and are distributed in living plant cells.…”

Section: Pol and Pll1 Are Lipid Binding Proteinsmentioning

confidence: 99%

TheArabidopsisStem Cell Factor POLTERGEIST Is Membrane Localized and Phospholipid Stimulated

Gagne

Clark

2010

The Plant Cell

View full text Add to dashboard Cite

Stem cell maintenance and differentiation are tightly regulated in multicellular organisms. In plants, proper control of the stem cell populations is critical for extensive postembryonic organogenesis. The Arabidopsis thaliana protein phosphatase type 2C proteins POLTERGEIST (POL) and PLL1 are essential for maintenance of both the root and shoot stem cells. Specifically, POL and PLL1 are required for proper specification of key asymmetric cell divisions during stem cell initiation and maintenance. POL and PLL1 are known to be integral components of the CLE/WOX signaling pathways, but the location and mechanisms by which POL and PLL1 are regulated within these pathways are unclear. Here, we show that POL and PLL1 are dual-acylated plasma membrane proteins whose membrane localization is required for proper function. Furthermore, this localization places POL and PLL1 in proximity of the upstream plasma membrane receptors that regulate their activity. Additionally, we find that POL and PLL1 directly bind to multiple lipids and that POL is catalytically activated by phosphatidylinositol (4) phosphate [PI(4)P] in vitro. Based on these results, we propose that the upstream receptors in the CLE/WOX signaling pathways may function to either limit PI(4)P availability or antagonize PI(4)P stimulation of POL/PLL1. Significantly, the findings presented here suggest that phospholipids play an important role in promoting stem cell specification.

show abstract

Imaging phosphatidylinositol 4‐phosphate dynamics in living plant cells

Cited by 158 publications

References 86 publications

Vesicle Dynamics during Plant Cell Cytokinesis Reveals Distinct Developmental Phases

Vesicle Dynamics during Plant Cell Cytokinesis Reveals Distinct Developmental Phases

Plasma Membrane Localization of Solanum tuberosum Remorin from Group 1, Homolog 3 Is Mediated by Conformational Changes in a Novel C-Terminal Anchor and Required for the Restriction of Potato Virus X Movement

TheArabidopsisStem Cell Factor POLTERGEIST Is Membrane Localized and Phospholipid Stimulated

Contact Info

Product

Resources

About