IgG subclass profiles in normal human sera of antibodies specific to five kinds of microbial antigens

Hjelholt, Astrid; Christiansen, Gunna; Sørensen, Uffe B. Skov; Birkelund, Svend

doi:10.1111/2049-632x.12034

Cited by 61 publications

(58 citation statements)

References 40 publications

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“…Rat IgG subtypes can be classified depending on their skew toward Th1 (IgG2b and IgG2c) or Th2 (IgG1 and IgG2a). Likewise, although human subtypes are not homologous to those in rat, they have been grouped depending on whether their production arises from Th1 (IgG1, IgG3, and probably IgG2) or Th2 (IgG4) responses [38,39]. Accordingly, whereas Th2-type Ig are minority, Th1-type are dominant in breast milk of both rats and humans (>85% of the total IgGs), indicating a similar immunological function of breast milk in both species [40][41][42].…”

Section: Discussionmentioning

confidence: 99%

Associations of Breast Milk Microbiota, Immune Factors, and Fatty Acids in the Rat Mother–Offspring Pair

et al. 2020

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The present study aimed to analyze the rat breast milk profile of fatty acids (FA), immunoglobulins (Ig), microbiota, and their relationship, and to further assess their associations in the mother–offspring pair. Dams were monitored during the three weeks of gestation, allowed to deliver at term, and followed during two weeks of lactation. At the end of the study, milk was obtained from the dams for the analysis of fatty acids, microbiota composition, immunoglobulins, and cytokines. Moreover, the cecal content and plasma were obtained from both the dams and pups to study the cecal microbiota composition and the plasmatic levels of fatty acids, immunoglobulins, and cytokines. Rat breast milk lipid composition was ~65% saturated FA, ~15% monounsaturated FA, and ~20% polyunsaturated FA. Moreover, the proportions of IgM, IgG, and IgA were ~2%, ~88%, and ~10%, respectively. Breast milk was dominated by members of Proteobacteria, Firmicutes, and Bacteroidetes phyla. In addition, forty genera were shared between the milk and cecal content of dams and pups. The correlations performed between variables showed, for example, that all IgGs subtypes correlated between the three compartments, evidencing their association in the mother-milk-pup line. We established the profile of FA, Ig, and the microbiota composition of rat breast milk. Several correlations in these variables evidenced their association through the mother-milk-pup line. Therefore, it would be interesting to perform dietary interventions during pregnancy and/or lactation that influence the quality of breast milk and have an impact on the offspring.

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Section: Discussionmentioning

confidence: 99%

Associations of Breast Milk Microbiota, Immune Factors, and Fatty Acids in the Rat Mother–Offspring Pair

et al. 2020

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“…31 As the four IgG subclasses differ in their ability to activate complement, and bactericidal activity in different antigenic contexts, 32 monitoring IgG subclass usage is useful. Normally, IgG2 is produced in response to polysaccharide antigens, IgG1 and IgG3 produced in response to protein antigens, 33 whereas IgG4 serves a more regulatory function. 34 The data here show that after polysaccharide vaccination, IgG2 was the predominant subclass produced, but after conjugate vaccination, IgG1 was the predominant subclass produced (even in individuals primed with the polysaccharide vaccine).…”

Section: Discussionmentioning

confidence: 99%

BCR repertoire sequencing: different patterns of B‐cell activation after two Meningococcal vaccines

et al. 2015

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Next-generation sequencing was used to investigate the B cell receptor heavy chain transcript repertoire of different B cell subsets (naïve, marginal zone, IgM memory and IgG memory) at baseline, and of plasma cells 7 days following administration of serogroup ACWY meningococcal polysaccharide and protein-polysaccharide conjugate vaccines. Baseline B cell subsets could be distinguished from each other using a small number of repertoire properties (clonality, mutation from germ-line and complementarity-determining region 3 length) that were conserved between individuals. However, analyzing the complementarity-determining region 3 amino acid sequence (which is particularly important for antigen binding) of the baseline subsets showed few sequences shared between individuals. In contrast, day 7 plasma cells demonstrated nearly tenfold greater sequence sharing between individuals than the baseline subsets, consistent with the plasma cells being induced by the vaccine antigen, and sharing specificity for a more limited range of epitopes. By annotating plasma cell sequences based on IgG subclass usage and mutation, and also comparing them to the sequences of the baseline cell subsets, we were able to identify different signatures after the polysaccharide and conjugate vaccines. Plasma cells produced after conjugate vaccination were predominantly IgG1, and most related to IgG memory cells. In contrast, after polysaccharide vaccination, the plasma cells were predominantly IgG2, less mutated, and were equally likely to be related to marginal zone, IgM memory or IgG memory cells. High-throughput B cell repertoire sequencing thus provides a unique insight into patterns of B cell activation not possible from more conventional measures of immunogenicity.

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“…HBsAg specific IgG antibodies with neutralizing activity such as IgG1 and IgG3 are required for complete viral clearance from the host [59]. Increased HBsAg specific IgG3 subclass antibody responses is essential for the clearance of pathogen by macrophages because IgG3 antibodies are involved in complement activation and opsonisation of invading microorganisms [60].…”

Section: Discussionmentioning

confidence: 99%

Antibody Responses Specific to Hepatitis B Virus Vaccine in Children Exposed InUtero to Antiretroviral Therapy

2019

JCEI

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The use of antiretroviral (ARV) has been one of the most effective means of preventing vertical transmission of the human immunodeficiency virus (HIV) to exposed children born of HIV infected mothers. Nevertheless, responses to childhood vaccination against Hepatitis B virus (HBV) infections remain suboptimal in HIV exposed uninfected children irrespective of maternal ARV prophylaxis. In a cross-sectional study we have assessed the impact of in-utero exposure to ARV on paediatric HBV vaccination. Anti-HBV surface antigen specific antibodies (anti-HBs abs) were measured in plasma specimens from 44 healthy children unexposed to both HIV and ARV (HU), 25 HIV-exposed uninfected children naïve to intrauterine exposure to ARV (HEU.AR -), 29 ARV and HIV-exposed uninfected children during pregnancy (HEU.ARV + ), 50 children vertically infected with HIV but naïve to intrauterine exposure to ARV (HEI.ARV -) and 22 children vertically infected with HIV with in utero exposure to ARV (HEI.ARV + ). The protective seroconversion rate after childhood HBV vaccination (anti-HBs ≥10 mUI/ml) among HEU.ARV + children (58%) was significantly lower relative to both HEU.ARVc -(100%, P=0.0010) and the healthy unexposed children (92 %, P=0.0069). Similarly, HEI.ARV + children also had significantly lower anti-HBs IgM antibody responses when compared to both HU (p=0.0003) and HEI.ARV -(0.0001) children respectively. Thus in-utero exposure to ARV probably contributes in reducing HBV vaccine antibody response rate in both HIV exposed uninfected and vertically infected children after childhood vaccination. Nevertheless, the overall impact of ARV was to improve anti-HBs IgG responses in HIV infected children suggesting a possible role in immune reconstitution leading to improved IgG antibody responses.

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IgG subclass profiles in normal human sera of antibodies specific to five kinds of microbial antigens

Cited by 61 publications

References 40 publications

Associations of Breast Milk Microbiota, Immune Factors, and Fatty Acids in the Rat Mother–Offspring Pair

Associations of Breast Milk Microbiota, Immune Factors, and Fatty Acids in the Rat Mother–Offspring Pair

BCR repertoire sequencing: different patterns of B‐cell activation after two Meningococcal vaccines

Antibody Responses Specific to Hepatitis B Virus Vaccine in Children Exposed InUtero to Antiretroviral Therapy

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