Identifying Resistance Gene Analogs Associated With Resistances to Different Pathogens in Common Bean

López, Camilo; Acosta, Iván F.; Jara, Carlos G.; Pedraza, Fabio; Gaitán-Solís, Eliana; Gallego, Gerardo; Beebe, Steve; Tohmé, Joe

doi:10.1094/phyto.2003.93.1.88

Cited by 105 publications

(123 citation statements)

References 52 publications

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“…Indeed, on the basis of comparative mapping studies, the region containing P-PRLJ1.a and -f (block 2 on Figure 1B) is known to harbor the Co-RVI anthracnose resistance gene (Adam-Blondon et al 1994;Freyre et al 1998). Altogether, the end of the short arm of chromosome 4 carries an important collection of specific R genes and R QTL effective against diverse pathogens [fungi C. lindemuthianum and Uromyces appendiculatus, bacteria Pseudomonas syringae pv phaseolicola, and bean golden yellow mosaic virus (BGYMV)] (Lopez et al 2003;Miklas et al 2006;Rodriguez-Suarez et al 2008). However, their location relative to each other is not precisely known because they are often present in different genotypes.…”

Section: Discussionmentioning

confidence: 99%

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

et al. 2009

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In common bean, the B4 disease resistance (R) gene cluster is a complex cluster localized at the end of linkage group (LG) B4, containing at least three R specificities to the fungus Colletotrichum lindemuthianum. To investigate the evolution of this R cluster since the divergence of Andean and Mesoamerican gene pools, DNA sequences were characterized from two representative genotypes of the two major gene pools of common bean (BAT93: Mesoamerican; JaloEEP558: Andean). Sequences encoding 29 B4-CC nucleotide-binding-site-leucine-rich-repeat (B4-CNL) genes were determined-12 from JaloEEP558 and 17 from BAT93. Although sequence exchange events were identified, phylogenetic analyses revealed that they were not frequent enough to lead to homogenization of B4-CNL sequences within a haplotype. Genetic mapping based on pulsed-field gel electrophoresis separation confirmed that the B4-CNL family is a large family specific to one end of LG B4 and is present at two distinct blocks separated by 26 cM. Fluorescent in situ hybridization on meiotic pachytene chromosomes revealed that two B4-CNL blocks are located in the subtelomeric region of the short arm of chromosome 4 on both sides of a heterochromatic block (knob), suggesting that this peculiar genomic environment may favor the proliferation of a large R gene cluster.

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Section: Discussionmentioning

confidence: 99%

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

et al. 2009

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“…In earlier studies, co-amplification of non-specific fragments apart from the expected amplicons has been observed in crops such as soybean (Yu et al, 1996), rice (Mago et al, 1999), grapes (Di Gaspero and Cipriani, 2002), and sorghum (Totad et al, 2005). DNA fragments of 500-600 bp were considered to be specific for RGA amplicons as earlier attempts with 300 and 900 bp did not produce any significant homology to known R-genes (Aarts et al, 1998;Lopez et al, 2003;Totad et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of NBS-LRR- resistance gene candidates in turmeric(Curcuma longa cv. surama)

Joshi¹,

Mohanty²,

Subudhi³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Turmeric (Curcuma longa), an important asexually reproducing spice crop of the family Zingiberaceae is highly susceptible to bacterial and fungal pathogens. The identification of resistance gene analogs holds great promise for development of resistant turmeric cultivars. Degenerate primers designed based on known resistance genes (R-genes) were used in combinations to elucidate resistance gene analogs from Curcuma longa cultivar surama. The three primers resulted in amplicons with expected sizes of 450-600 bp. The nucleotide sequence of these amplicons was obtained through sequencing; their predicted amino acid sequences compared to each other and to the amino acid sequences of known R-genes revealed significant sequence similarity. The finding of conserved domains, viz., kinase-1a, kinase-2 and hydrophobic motif, provided evidence that the sequences belong to the NBS-LRR class gene family. The presence of tryptophan as the last residue of kinase-2 motif further qualified them to be in the non-TIR-NBS-LRR subfamily of resistance genes. A cluster analysis based on the neighbor-joining method was carried out using Curcuma NBS analogs together with several resistance gene analogs and known R-genes, 1797©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (3): 1796-1806 (2010) Isolation and characterization of Curcuma NBS analogs which classified them into two distinct subclasses, corresponding to clades N3 and N4 of non-TIR-NBS sequences described in plants. The NBS analogs that we isolated can be used as guidelines to eventually isolate numerous R-genes in turmeric.

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“…A cluster of germin-like proteins confers resistance to rice blast and sheath blight of rice (Manosalva et al 2009). Similarly, resistance gene homologs, which are known to colocalize with broad-spectrum disease resistance loci, can cluster in the genome and contribute a diversity of specificities (Lopez et al 2003;Ramalingam et al 2003). Pleiotropy remains uncommon in maize, and correlated responses may be due to While in some cases single genes or alleles common across diverse germplasm confer disease resistance, increasingly, the role of structural variation in plants is being explored and its effects on phenotypic variation recognized (Springer et al 2009;Chia et al 2012;McHale et al 2012).…”

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confidence: 99%

“…A cluster of germin-like proteins confers resistance to rice blast and sheath blight of rice (Manosalva et al 2009). Similarly, resistance gene homologs, which are known to colocalize with broad-spectrum disease resistance loci, can cluster in the genome and contribute a diversity of specificities (Lopez et al 2003;Ramalingam et al 2003). Pleiotropy remains uncommon in maize, and correlated responses may be due to linkage or population structure (Wallace et al 2014), although in some cases, individual genes have been shown to condition multiple disease resistance (MDR).…”

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confidence: 99%

Unraveling Genomic Complexity at a Quantitative Disease Resistance Locus in Maize

et al. 2014

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Multiple disease resistance has important implications for plant fitness, given the selection pressure that many pathogens exert directly on natural plant populations and indirectly via crop improvement programs. Evidence of a locus conditioning resistance to multiple pathogens was found in bin 1.06 of the maize genome with the allele from inbred line "Tx303" conditioning quantitative resistance to northern leaf blight (NLB) and qualitative resistance to Stewart's wilt. To dissect the genetic basis of resistance in this region and to refine candidate gene hypotheses, we mapped resistance to the two diseases. Both resistance phenotypes were localized to overlapping regions, with the Stewart's wilt interval refined to a 95.9-kb segment containing three genes and the NLB interval to a 3.60-Mb segment containing 117 genes. Regions of the introgression showed little to no recombination, suggesting structural differences between the inbred lines Tx303 and "B73," the parents of the fine-mapping population. We examined copy number variation across the region using next-generation sequencing data, and found large variation in read depth in Tx303 across the region relative to the reference genome of B73. In the fine-mapping region, association mapping for NLB implicated candidate genes, including a putative zinc finger and pan1. We tested mutant alleles and found that pan1 is a susceptibility gene for NLB and Stewart's wilt. Our data strongly suggest that structural variation plays an important role in resistance conditioned by this region, and pan1, a gene conditioning susceptibility for NLB, may underlie the QTL.T HE genes and loci that influence host-pathogen interactions vary in allele effects, specificities, and linkage relationships. While disease resistance can be conditioned by single genes with large effect (Bent 1996;Jones and Dangl 2006), the emerging model of resistance for many plant diseases is complex in nature, with many genes and loci functioning in concert and each contributing a small proportion of the total phenotypic variation Poland et al. 2011;Cook et al. 2012b). Each locus has a unique profile, with some loci contributing broadspectrum protection against diverse pathogen species and strains. Investigating these intricacies offers the opportunity to understand the diverse ways in which plants defend themselves against microbial assault.Correlated responses to multiple diseases have been observed in various germplasm panels, implying that there are loci and genes that condition broad-spectrum resistance (Rossi et al. 2006;Gurung et al. 2009;Wisser et al. 2011). At the chromosomal segment level, disease and insect resistance loci colocalize in a nonrandom fashion (McMullen and Simcox 1995;Williams 2003;Wisser et al. 2005) and loci have been identified that confer resistance to diverse pathogen isolates and taxa (Zwonitzer et al. 2010;Chung et al. 2011;Belcher et al. 2012). There is evidence to suggest that gene clusters can confer resistance to more than one disease. A cluster of germin-like ...

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Identifying Resistance Gene Analogs Associated With Resistances to Different Pathogens in Common Bean

Cited by 105 publications

References 52 publications

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

Isolation and characterization of NBS-LRR- resistance gene candidates in turmeric(Curcuma longa cv. surama)

Unraveling Genomic Complexity at a Quantitative Disease Resistance Locus in Maize

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