Identifying cotton (Gossypium hirsutum L.) genes induced in response to Aspergillus flavus infection

Lee, Seokhyun; Rajasekaran, Kanniah; Ramanarao, Mangu Venkata; Bedre, Renesh; Bhatnagar, Deepak; Baisakh, Niranjan

doi:10.1016/j.pmpp.2012.08.001

Cited by 9 publications

(17 citation statements)

References 39 publications

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“…ACP systems have been widely used in differential gene expression studies since the development of improved systems and commercially available kits (Kim et al 2004 ). Although more studies have been reported for animals, the ACP differential display has been successfully used for identification of genes involved in spine formation on seeds of carrots (Park et al 2006 ), Cd-responsive genes in Solanum nigrum (Xu et al 2009 ), drought induced genes in barley (Lee et al 2011 ) and induced genes in cotton after inoculation with Aspergillus flavus (Lee et al 2012 ). We found ACP-based differential display more advantageous than cDNA-AFLP because of its methodological simplicity, due to the use of agarose gels instead of long AFLP polyacrylamide gels and due to obtaining much longer TDFs with good matches to known sequences.…”

Section: Resultsmentioning

confidence: 99%

Different Gene Expressions of Resistant and Susceptible Hop Cultivars in Response to Infection with a Highly Aggressive Strain of Verticillium albo-atrum

et al. 2014

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Verticillium wilt has become a serious threat to hop production in Europe due to outbreaks of lethal wilt caused by a highly virulent strain of Verticillium albo-atrum. In order to enhance our understanding of resistance mechanisms, the fungal colonization patterns and interactions of resistant and susceptible hop cultivars infected with V. albo-atrum were analysed in time course experiments. Quantification of fungal DNA showed marked differences in spatial and temporal fungal colonization patterns in the two cultivars. Two differential display methods obtained 217 transcripts with altered expression, of which 84 showed similarity to plant proteins and 8 to fungal proteins. Gene ontology categorised them into cellular and metabolic processes, response to stimuli, biological regulation, biogenesis and localization. The expression patterns of 17 transcripts with possible implication in plant immunity were examined by real-time PCR (RT-qPCR). Our results showed strong expression of genes encoding pathogenesis-related (PR) proteins in susceptible plants and strong upregulation of genes implicated in ubiquitination and vesicle trafficking in the incompatible interaction and their downregulation in susceptible plants, suggesting the involvement of these processes in the hop resistance reaction. In the resistant cultivar, the RT-qPCR expression patterns of most genes showed their peak at 20 dpi and declined towards 30 dpi, comparable to the gene expression pattern of in planta detected fungal protein and coinciding with the highest fungal biomass in plants at 15 dpi. These expression patterns suggest that the defence response in the resistant cultivar is strong enough at 20 dpi to restrict further fungus colonization.Electronic supplementary materialThe online version of this article (doi:10.1007/s11105-014-0767-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Different Gene Expressions of Resistant and Susceptible Hop Cultivars in Response to Infection with a Highly Aggressive Strain of Verticillium albo-atrum

et al. 2014

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“…Development of genetically edited crops is similar to those developed by conventional or mutation breeding and this potential technique is useful in providing sustainable productive agriculture for better feeding of rapidly growing population in a changing climate. Once the target host plant genes are identified through comparative transcriptomic and gene network analyses in susceptible crops (Bedre et al, 2015;Clevenger et al, 2016;Lee et al, 2012;Musungu et al, 2015) these modern tools can be applied for generating aflatoxin resistant crops.…”

Section: Molecular Breedingmentioning

confidence: 99%

Advances in molecular and genomic research to safeguard food and feed supply from aflatoxin contamination

Bhatnagar

Rajasekaran

Gilbert

et al. 2018

WMJ

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Worldwide recognition that aflatoxin contamination of agricultural commodities by the fungus Aspergillus flavus is a global problem has significantly benefitted from global collaboration for understanding the contaminating fungus, as well as for developing and implementing solutions against the contamination. The effort to address this serious food and feed safety issue has led to a detailed understanding of the taxonomy, ecology, physiology, genomics and evolution of A. flavus, as well as strategies to reduce or control pre-harvest aflatoxin contamination, including (1) biological control, using atoxigenic aspergilli, (2) proteomic and genomic analyses for identifying resistance factors in maize as potential breeding markers to enable development of resistant maize lines, and (3) enhancing host-resistance by bioengineering of susceptible crops, such as cotton, maize, peanut and tree nuts. A post-harvest measure to prevent the occurrence of aflatoxin contamination in storage is also an important component for reducing exposure of populations worldwide to aflatoxins in food and feed supplies. The effect of environmental changes on aflatoxin contamination levels has recently become an important aspect for study to anticipate future contamination levels. The ability of A. flavus to produce dozens of secondary metabolites, in addition to aflatoxins, has created a new avenue of research for understanding the role these metabolites play in the survival and biodiversity of this fungus. The understanding of A. flavus, the aflatoxin contamination problem, and control measures to prevent the contamination has become a unique example for an integrated approach to safeguard global food and feed safety.

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“…parasiticus [ 1 – 6 ]. Aflatoxin B1 is the most widely occurring structure that is carcinogenic to humans and animals [ 2 – 4 ]. Aflatoxins cause suppression of the immune system, cancer, retardation in growth, and in extreme cases death of both humans and animals.…”

Section: Introductionmentioning

confidence: 99%

“…Aflatoxins cause suppression of the immune system, cancer, retardation in growth, and in extreme cases death of both humans and animals. Aflatoxins have the ability to contaminate variety of crops such as corn, cotton, peanut and tree nuts during their growth and development, accounting to an estimated economic loss of ~$270 M annually worldwide [ 4 ], [ 5 ], [ 7 ]. The occurrence of aflatoxin in agricultural products is highly prohibited.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Transcriptome Analysis of Cotton (Gossypium hirsutum L.) Identifies Candidate Gene Signatures in Response to Aflatoxin Producing Fungus Aspergillus flavus

et al. 2015

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Aflatoxins are toxic and potent carcinogenic metabolites produced from the fungi Aspergillus flavus and A. parasiticus. Aflatoxins can contaminate cottonseed under conducive preharvest and postharvest conditions. United States federal regulations restrict the use of aflatoxin contaminated cottonseed at >20 ppb for animal feed. Several strategies have been proposed for controlling aflatoxin contamination, and much success has been achieved by the application of an atoxigenic strain of A. flavus in cotton, peanut and maize fields. Development of cultivars resistant to aflatoxin through overexpression of resistance associated genes and/or knocking down aflatoxin biosynthesis of A. flavus will be an effective strategy for controlling aflatoxin contamination in cotton. In this study, genome-wide transcriptome profiling was performed to identify differentially expressed genes in response to infection with both toxigenic and atoxigenic strains of A. flavus on cotton (Gossypium hirsutum L.) pericarp and seed. The genes involved in antifungal response, oxidative burst, transcription factors, defense signaling pathways and stress response were highly differentially expressed in pericarp and seed tissues in response to A. flavus infection. The cell-wall modifying genes and genes involved in the production of antimicrobial substances were more active in pericarp as compared to seed. The genes involved in auxin and cytokinin signaling were also induced. Most of the genes involved in defense response in cotton were highly induced in pericarp than in seed. The global gene expression analysis in response to fungal invasion in cotton will serve as a source for identifying biomarkers for breeding, potential candidate genes for transgenic manipulation, and will help in understanding complex plant-fungal interaction for future downstream research.

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Identifying cotton (Gossypium hirsutum L.) genes induced in response to Aspergillus flavus infection

Cited by 9 publications

References 39 publications

Different Gene Expressions of Resistant and Susceptible Hop Cultivars in Response to Infection with a Highly Aggressive Strain of Verticillium albo-atrum

Different Gene Expressions of Resistant and Susceptible Hop Cultivars in Response to Infection with a Highly Aggressive Strain of Verticillium albo-atrum

Advances in molecular and genomic research to safeguard food and feed supply from aflatoxin contamination

Genome-Wide Transcriptome Analysis of Cotton (Gossypium hirsutum L.) Identifies Candidate Gene Signatures in Response to Aflatoxin Producing Fungus Aspergillus flavus

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