Identifikasi Keragaman Gen DGAT1 serta Asosiasinya terhadap Karakteristik Karkas dan Sifat Perlemakan Domba

Abstract

ABSTRAK Karakteristik karkas dan sifat perlemakan pada daging domba dikontrol oleh banyak gen salah satunya gen DGAT1 (Diacylglycerol Acyltransferasel 1). Penelitian ini bertujuan mengidentifikasi SNP (Single Nucleotide Polymorphism) gen DGAT1 pada titik mutasi g.8539 C>T dan asosiasinya terhadap karakteristik karkas dan sifat perlemakan pada domba Indonesia. Total sampel domba yang digunakan sebanyak 150 buah terdiri dari 35 sampel domba compass agrinak (DCA), 36 sampel domba barbados cross (DBC), 41 sampel domba komposit garut (DKG), 20 sampel domba ekor gemuk (DEG), dan 18 sampel domba ekor tipis (DET). Karakteristik karkas dan sifat perlemakan diukur dari domba jantan berumur 10-12 bulan. Identifikasi keragaman DGAT1|ALuI dianalisis dengan metode PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). Hasil keragaman gen DGAT1 bersifat polimorfik dalam DET dan DEG, sedangkan DCA, DBC, dan DKG bersifat monomorfik. Dua genotipe disebut CC dan CT ditemukan dalam DET dan DEG. Titik mutasi gen DGAT1 berasosiasi (P<0.05) dengan karakteristik karkas, yaitu bobot dan panjang karkas. Selain itu, keragaman gen DGAT1 juga berasosiasi signifikan (P<0.05) dengan asam lemak jenuh, yaitu asam stearat (C18:0) dan asam arakidat (C20:0) dan asam lemak tak jenuh tunggal, yaitu asam oleat (C18:1n9c). Gen DGAT1 memiliki kontribusi dalam karakteristik karkas dan komposisi asam lemak pada domba.Kata Kunci: domba, gen DGAT1, karakteristik karkas, PCR-RFLP, sifat perlemakan ABSTRACT Characteristic of carcass and fatness traits of sheep is regulated by many genes such as DGAT1 (Diacylglycerol Acyltransferasel 1) gene. The research was aimed to investigate SNP (Single Nucleotide Polymorphism) of DGAT1 and its association with characteristic of carcass and fatness traits in Indonesian sheep. A total sample of sheeps used 150 rams of 10–12 months consisted 35 samples of compas agrinak sheep (CAS), 36 of barbados cross (BCS), 41 of garut composite (GCS), 20 of javanese fat tailed (JFT), and 18 of javanese thin tailed (JTT). Identification variant of DGAT1|ALuI were performed by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). The results of polymorphism of DGAT1 were found in JTT and JFT. However, SNP of DGAT1 in CAS, BCS and GCS were monomorfic. Two genotype namely CC and CT were found in JTT and JFT populations. A SNP of the DGAT1 was associated (P<0.05) with characteristic of carcass, including weight and length of carcass. The variant of DGAT1 was associated too with saturated fatty acids (SFA) including stearic acid (C18:0) and arachidic acid (C20:0), and mono unsaturated fatty acid (MUFA) including oleic acid (C18:1n9c). The DGAT1 gene was contribute to characteristic carcass and fatty acid composition in sheep.Keywords: DGAT1 gene, characteristic carcass, fatness traits, PCR-RFLP, sheep

Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep

“…The CT and TT genotype were associated with higher level of carcass length. These findings are in consistent with a previous study performed with a different gene (DGAT1) in Indonesian sheep [27].The leg has a higher weight of muscle, bone, and subcutaneous fat compared to other parts (shoulder, shank, breast, neck, rack, loin, flank, and tenderloin). On the other hand, the leg has a lower intramuscular fat than the shoulder.…”

Identification of Single Nucleotide Polymorphism and Association Analysis of Alpha 2-Heremans Schmid Glycoprotein (AHSG) Gene Related to Fatty Acid Traits in Sheep

“…The AHSG gene single nucleotide polymorphism (G>A) is located at the nucleotide position g.198655287, exon 2 of chromosome 1 with GenBank Numbers shown in parentheses are the number of individuals with the specified genotype. 1) MUFA: monounsaturated fatty acid; 2) C17:1: heptadecenoic acid; 3) C20:1: gondoic acid (Eicosenoic acid); 4) PUFA: polyunsaturated fatty acid , 5) C18:3n6: γ-Linolenic acid; 6) C18:3n3: α-Linolenic acid; 7) DGLA: dihomogamma-linolenic acid; 8) AA: arachidonic acid; 9) DPA : docosapentaenoic acid (eicosapentanoic); 10) DHA: docosahexaenoic acid).…”

“…Note: a, b,c,d Mean value with different superscript letters in the same row differ significant at P<0.05.Numbers shown in parentheses are the number of individuals with the specified genotype 1). MUFA: Monounsaturated fatty acid; 2) C17:1: Heptadecenoic acid; 3) C20:1: Gondoic acid (Eicosenoic acid); 4) PUFA: polyunsaturated fatty acid ,5) C18:3n6: γ-Linolenic acid; 6) C18:3n3: α-Linolenic acid;7) DGLA: Dihomogamma-linolenic acid; 8) AA: Arachidonic acid;9) DPA : DPA docosapentaenoic acid (Eicosapentanoic);10) DHA: Docosahexaenoic acid).The g.198655287 (G>A) SNP of AHSG gene was significant associated with fatty acids traits in combined population as shown in Table2. There was a significant association with saturated fatty acids including, capric acid (C10:0), tridecylic acid (C13:0), myristic acid (C14:0), pentadecanoic acid (C15:0), palmitic acid (C16:0), palmitic acid (C16:0), heptadecanoic acid (17:0), stearic acid (C18:0), arachidic acid (C20:0), heneicosylic acid (C21:0), behenic acid (C22:0), tricosylic acid (C23:0) and lignoceric acid (C24:0), monounsaturated fatty acids including myristoleic acid (C14:1), heptadecenoic acid, elaidic acid(C18:1n9t, oleic acid (C18:1n9c), oleic acid (C18:1n9c), eicosenoic acid and nervonic acid (C24:1).…”

Association of Cytochrome P450 2A6 (CYP2A6) Gene Polymorphisms with Fatty Acid Traits in Indonesian Native Sheep

“…Several genes were reported to be significantly associated with fatty acid content in sheep including APOA5 (Gunawan et al, 2018b), AHSG (Munyaneza et al, 2019a), BHMT (Munyaneza et al, 2019b), DGAT1 (Gunawan et al, 2019), and KIF12 (Gunawan et al, 2018a). Cytochrome P450 2A6 (CYP2A6) are genes thought to contribute in the fatty acid traits in sheep.…”