Identifikasi Bakteri Escherichia Coli O157:h7 Pada Feses Penderita Diare Dengan Metode Kultur Dan PCR

Rizky, Vincentia Ade; Siregar, Saadah; Krisdianilo, Visensius; Rahayu, Asvia; Ginting, Suventi Syafrina; Kartini, .

doi:10.35451/jfm.v3i2.615

Cited by 6 publications

(6 citation statements)

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“…Sebanyak 1.493 (17%) kasus, penderit masuk rumah sakit, 254 (4%) penderita teridentifikasi mengalami HUS (Hemolytic Uremic Syndrome), dan 40 (0,5%) kasus penderita meninggal. 7 Menurut penelitian Endah prayekti (2017) yang dilakukan di Sekolah Dasar pada wilayah Jemurwonosari Surabaya terdapat seluruh jajanan minuman terkontaminasi Esherichia Coli. 8 Menurut penelitian zafrida siska (2022) pada jajanan makanan yang dijual di kantin SDN 165 Dan SDN 176 Kelurahan Tobek Godang Kecematan Tampan Pekanbaru ditemukan adanya cemaran Esherichia coli.…”

Section: Pendahuluanunclassified

Gambaran Cemaran Bakteri Escherichia coli pada Jajanan di SDN 70 Banda Aceh

Safira,

Rahmayanti,

Auliani

2023

MKMI

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Latar belakang: Anak usia sekolah umumnya setiap hari menghabiskan sepertiga waktunya di sekolah, sehingga menyebabkan mereka menghabiskan waktu makan siangnya di sekolah. Adapun jenis jajanan yang dijajakan beraneka ragam dari mulai jajanan sehat maupun jajanan tidak sehat. Makanan sehat yang mempunyai gizi yang cukup, sedangkan makanan tidak sehat sudah terkontaminasi mikroba. Salah satu mikroba yang berbahaya bagi manusia pada makanan adalah bakteri Escherichia coli. Tujuan penelitian ini untuk mengetahui apakah terdapat cemaran bakteri Escherichia coli pada jajanan di SDN 70 Banda Aceh.Metode: Metode jenis penelitian ini adalah deskriptif. Penelitian ini menggunkana metode kuantitifikasi bakteri dengan Teknik MPN (Most Probable Number) dan TPC (Total Plate Count). Sampel dipilih dengan metode purposive sampling.Hasil: Hasil penelitian menunjukkan bahwa 3 sampel minuman yang diuji dengan metode MPN (Most Probable Number) melebihi batas maksimum. Sedangkan 6 sampel makanan yang diuji dengan meode TPC (Total Plate Count) dinyatakan masih dalam ambang batas.Simpulan: Hasil penelitian menunjukkan bahwa semua sampel minuman terkontaminasi bakteri Escherichia coli dan unutk makanan tidak terkontaminasi bakteri Escherichia coli.Kata kunci: jajanan sekolah, kontaminasi, Escherichia coli Title: Description of Contamination of Escherichia Coli Bacteria in Food at SDN 70 Banda AcehBackground: School-age children generally spend a third of their time at school every day, causing them to spend their lunch time at school. The types of snacks that are sold vary from healthy snacks to unhealthy snacks. Healthy food that has sufficient nutrition, while unhealthy food is contaminated with microbes. One of the microbes that are harmful to humans in food is Escherichia coli bacteria. The purpose of this study was to determine whether there was contamination of Escherichia coli bacteria in snacks at SDN 70 Banda Aceh.Method: This type of research method is descriptive. This study used a bacterial quantification method using MPN (Most Probable Number) and TPC (Total Plate Count) techniques. The sample was selected by purposive sampling method.Result: The results showed that 3 samples of drinks tested using the MPN (Most Probable Number) method exceeded the maximum limit. while 6 food samples tested with the TPC (Total Plate Count) method were declared to be still within the threshold. Conclusion: Showed that all drink samples were contaminated with Escherichia coli bacteria and food was not contaminated with Escherichia coli bacteria.Keywords: school snacks, contamination, Escherichia coli

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Section: Pendahuluanunclassified

Gambaran Cemaran Bakteri Escherichia coli pada Jajanan di SDN 70 Banda Aceh

Safira,

Rahmayanti,

Auliani

2023

MKMI

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“…Dari agent-agent diare tersebut bakteri Escherichia coli merupakan pernyebab diare terbanyak kedua setelah rotavirus. 7,8 Penyebab diare akibat bakteri Escherichia coli yang paling sering terjadi disebabkan oleh galur Diarrheagenic Escherichia coli (DEC) yang memiliki beberapa subtipe. Diantara subtipenya, Enteropathogenic Escherichia coli (EPEC) dan Enteroaggregative Escherichia coli (EAEC) yang sangat patogen dan kasus diare yang disebabkan subtipe ini sering terjadi di negara berkembang.…”

Section: Pendahuluanunclassified

IDENTIFIKASI BAKTERI Escherichia coli SUBTIPE Enteroaggregative Escherichia coli DAN Enteropathogenic Escherichia coli DENGAN METODE KULTUR DAN POLYMERASE CHAIN REACTION PADA SATE DAGING BABI DI KOTA DENPASAR

Putra,

Sukrama,

Budayanti

et al. 2022

eum

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ABSTRACT Pork satay is a traditional Balinese dish whose basic ingredient is pork. Pork contains Escherichia coli bacteria which is a normal flora in pigs, so if it is not processed properly and hygienically, it has the potential to be contaminated with Escherichia coli bacteria from pork or in the processing process which can cause diarrhea if consumed. This study aims to identify the presence of Escherichia coli bacteria contamination as well EAEC and EPEC subtypes on pork satay in Denpasar City. This research is a descriptive study with an observational research design. In this study, using cluster purposive sampling technique with 16 samples taken from each sub-district in Denpasar City. All samples were examined at the Microbiology Laboratory, Faculty of Medicine, Udayana University. The samples were cultured on Eosin Methylene Blue Agar (EMBA) media and the positive results of Escherichia coli bacteria from the culture process were continued to the identification stage using the Polymerase Chain Reaction (PCR) method. Of the 16 samples of pork satay tested for culture, 9 (56%) samples were positive for Escherichia coli bacteria. All positive samples were continued with the identification process using the Polymerase Chain Reaction (PCR) method and the results showed no one of EAEC and EPEC subtypes were detected in all positive samples. In this study, most of the pork satay samples were contaminated with Escherichia coli bacteria, but after molecular identification, no one of EAEC and EPEC subtypes were found in all samples that were positive for Escherichia coli bacteria. Keywords : Pork satay, Escherichia coli, Diarrhea

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“…Infectious diseases are still the main cause of high morbidity and mortality in the world (Sachwiver, Surya, & Elianora, 2018). The types of bacteria that most often infect humans are Staphylococcus aureus Sp (Rizky et al, 2021) and Escherichia coli (Rizky, Agrijanti, & Inayati, 2019).…”

Section: Introductionmentioning

confidence: 99%

Formulation of Lahuna Leave (Eupatorium odoratum) and Sirih Leave Extract (Piper betle L.) as Antiseptic Liquid Soap

Jumrah¹,

Abubakar²,

Agustina³

et al. 2023

Indo. J. Chem. Res.

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Infectious diseases caused by microorganisms are the main cause of high morbidity and mortality in the world. One of the plants that have the potential as an antiseptic is a lahuna leaf. Lahuna leaves contain active antibacterial compounds and the addition of betel leaf to the liquid soap formulations can strengthen the activity of the antiseptic produced. The purpose of this research is to test the effectiveness of liquid soap formulations of lahuna leaves and betel leaves as an antiseptic. The methods used include phytochemical screening, physical observation of liquid soap formulations, and antibacterial activity tests using agar diffusion methods. The results of the phytochemical analysis showed that lahuna leaves contain flavonoids, tannins, terpenoids, and alkaloids, while betel leaves contain flavonoids, saponins, tannins, terpenoids, and alkaloids. The liquid soap formulation of lahuna leaves and betel leaves has a clear yellow color, distinctive aroma, liquid form, rough taste, and lots of foam and has very strong inhibition against Staphylococcus aureus which is characterized by clear zones formed for each formulation I (22.4 mm), formulation II (21.8 mm) and formulation III (20.1 mm). These results indicate the potential of liquid soap formulations of lahuna leaves and betel leaves as antiseptic soap.

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Identifikasi Bakteri Escherichia Coli O157:h7 Pada Feses Penderita Diare Dengan Metode Kultur Dan PCR

Cited by 6 publications

References 0 publications

Gambaran Cemaran Bakteri Escherichia coli pada Jajanan di SDN 70 Banda Aceh

Gambaran Cemaran Bakteri Escherichia coli pada Jajanan di SDN 70 Banda Aceh

IDENTIFIKASI BAKTERI Escherichia coli SUBTIPE Enteroaggregative Escherichia coli DAN Enteropathogenic Escherichia coli DENGAN METODE KULTUR DAN POLYMERASE CHAIN REACTION PADA SATE DAGING BABI DI KOTA DENPASAR

Formulation of Lahuna Leave (Eupatorium odoratum) and Sirih Leave Extract (Piper betle L.) as Antiseptic Liquid Soap

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