Identification of two RNA silencing suppressors from banana bunchy top virus

Niu, Shengniao; Wang, Baosheng; Guo, Xinglin; Yu, Jialin; Wang, Xianbing; Xu, Kai; Zhai, Yi; Wang, Jianhua; Liu, Zhixin

doi:10.1007/s00705-009-0515-5

Cited by 25 publications

(26 citation statements)

References 52 publications

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“…The results of our study contrast with those of Niu et al [57]. They reported that the gene products encoded by DNA 3 (DNA-S; CP) and DNA 4 (DNA-M; MP) of a BBTV isolate from Hainan can suppress RNA silencing, whereas we have shown that DNA-M and DNA-C encoded genes have suppressor activity.…”

Section: Discussioncontrasting

confidence: 86%

“…One possible reason is that the isolate of BBTV we have examined belongs to the ''South-Pacific'' group [31], whereas the Hainan isolate belongs to the ''Asian'' group [58]. Phylogenetic analyses have shown that these groups of isolates are evolutionarily distinct [57] and upon divergence the two groups may have evolved to use distinct proteins as suppressors of RNA silencing. The predicted amino acid sequences of the Clink from our study and other ''South-Pacific'' BBTV isolates are distinct from those of typical ''Asian'' isolates (Fig.…”

Section: Discussionmentioning

confidence: 92%

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Identification of a major pathogenicity determinant and suppressors of RNA silencing encoded by a South Pacific isolate of Banana bunchy top virus originating from Pakistan

et al. 2010

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Five genes encoded by Banana bunchy top virus (BBTV) originating from Pakistan were expressed in Nicotiana benthamiana using a Potato virus X (PVX) vector. Expression of the master replication-associated protein (mRep) and movement protein (MP) resulted in necrotic cell death of inoculated tissues, as well as leaf curling and necrosis along the veins in newly emerging leaves. The systemic necrosis induced by the expression of MP was discolored (dark) in comparison to that induced by mRep. Expression of the cell-cycle link protein (Clink), the coat protein (CP), and the nuclear shuttle protein from the PVX vector induced somewhat milder symptoms, consisting of mild leaf curling and mosaic, although expression of the CP caused a necrotic response in inoculated leaf. The accumulation of viral RNA was enhanced by MP, Clink, and CP. Of the five BBTV-encoded gene products two, the MP and Clink, stabilized GFP-specific mRNA and reduced GFP-specific small interfering RNA in N. benthamiana line 16c when expressed under the control of the 35S promoter and co-inoculated with a construct for the expression of GFP hairpin RNA construct. These results identified MP and Clink as suppressors of RNA silencing. Taken together the ability of MP to induce severe symptoms in plants and suppress RNA silencing implicates this product as a major pathogenicity determinant of BBTV.

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Section: Discussioncontrasting

confidence: 86%

Section: Discussionmentioning

confidence: 92%

Identification of a major pathogenicity determinant and suppressors of RNA silencing encoded by a South Pacific isolate of Banana bunchy top virus originating from Pakistan

et al. 2010

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“…In addition, we showed that HVT063 could reverse GFP silencing of infiltrated patches but not suppressed systemic gene silencing in newly emerging leaves. Similarly, B3 protein of Banana bunchy top virus also only recovered GFP fluorescence again in the inoculated leaves (39), while PVY HC-Pro could restore the GFP fluorescence of silenced transgenic plants in both old and new leaves and CMV 2b rescues GFP expression only in new emerging leaves (32). A possible clue accounting for this result is that HVT063 may directly interact with the essential components of RNA silencing pathway, which is dosage dependent, or this kind of suppression could not be transferred to remote tissues.…”

Section: Discussionmentioning

confidence: 92%

A Multifunctional Protein Encoded by Turkey Herpesvirus Suppresses RNA Silencing in Nicotiana benthamiana

Xia

Fan

Jia

et al. 2011

J Virol

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Many plant and animal viruses counteract RNA silencing-mediated defense by encoding diverse RNA silencing suppressors. We characterized HVT063, a multifunctional protein encoded by turkey herpesvirus (HVT), as a silencing suppressor in coinfiltration assays with green fluorescent protein transgenic Nicotiana benthamiana line 16c. Our results indicated that HVT063 could strongly suppress both local and systemic RNA silencing induced by either sense RNA or double-stranded RNA (dsRNA). HVT063 could reverse local silencing, but not systemic silencing, in newly emerging leaves. The local silencing suppression activity of HVT063 was also verified using the heterologous vector PVX. Further, single alanine substitution of arginine or lysine residues of the HVT063 protein showed that each selected single amino acid contributed to the suppression activity of HVT063 and region 1 (residues 138 to 141) was more important, because three of four single amino acid mutations in this region could abolish the silencing suppressor activity of HVT063. Moreover, HVT063 seemed to induce a cell death phenotype in the infiltrated leaf region, and the HVT063 dilutions could decrease the silencing suppressor activity and alleviate the cell death phenotype. Collectively, these results suggest that HVT063 functions as a viral suppressor of RNA silencing that targets a downstream step of the dsRNA formation in the RNA silencing process. Positively charged amino acids in HVT063, such as arginine and lysine, might contribute to the suppressor activity by boosting the interaction between HVT063 and RNA, since HVT063 has been demonstrated to be an RNA binding protein.Small interfering RNA (siRNA)-guided gene silencing serves as a key component of host defense strategy against viruses in plants, invertebrates, and fungi, as well as higher animals (2,5,9,20). RNA silencing refers to the suppression of gene expression through homology-dependent mRNA degradation, and it is thought to be initiated by double-stranded RNA (dsRNA) molecules (21). The dsRNA is recognized and processed by Dicer or Dicer-like proteins into small RNA duplexes of 21 to 24 nucleotides (nt) (5), and one strand of the duplex is subsequently incorporated into a multisubunit endonuclease called the RNA-induced silencing complex (RISC) that initiates the sequence-specific degradation of target RNAs (48).To counteract host antiviral defenses, viruses have evolved sophisticated mechanisms, including encoding proteins that are capable of suppressing the RNA silencing process (52). The first silencing suppressor was discovered in plant virus, and then different suppressor proteins were identified in many plant viruses, as well as animal viruses (3). The discovery of RNA silencing suppressor (RSS) functions in animal viruses provided evidence of conserved RNA silencing pathway in the plant and animal kingdoms (45,59,61). Up to now, more than 70 RSSs have been found; however, less than 15 animal proteins have been identified as RSSs. The RSSs are extremely diverse within and across king...

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“…CP-mediated resistance was one of the most common pathogen derived resistance strategies employed against plant viruses and has proven success against many viruses [15]. However CP mediated resistance may only be effective against closely related viruses which share a high level of sequence homology [1,4,19,34,35]. High level of conservation of 96-100 % observed in the CP gene of BBTV isolates of South Pacific group analyzed in the present study suggests that any BBTV CP transgene on the South Pacific isolates likely to provide effective CP mediated resistance against South Pacific BBTV isolates including Sri Lankan isolate.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

et al. 2016

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Bunchy top disease of banana caused by Banana bunchy top virus (BBTV, genus Babuvirus family Nanoviridae) is one of the most important constraints in production of banana in the different parts of the world. Six genomic DNA components of BBTV isolate from Kandy, Sri Lanka (BBTV-K) were amplified by polymerase chain reaction (PCR) with specific primers using total DNA extracted from banana tissues showing typical symptoms of bunchy top disease. The amplicons were of expected size of 1.0-1.1 kb, which were cloned and sequenced. Analysis of sequence data revealed the presence of six DNA components; DNA-R, DNA-U3, DNA-S, DNA-N, DNA-M and DNA-C for Sri Lanka isolate. Comparisons of sequence data of DNA components followed by the phylogenetic analysis, grouped Sri Lanka-(Kandy) isolate in the Pacific Indian Oceans (PIO) group. Sri Lanka-(Kandy) isolate of BBTV is classified a new member of PIO group based on analysis of six components of the virus.

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Identification of two RNA silencing suppressors from banana bunchy top virus

Cited by 25 publications

References 52 publications

Identification of a major pathogenicity determinant and suppressors of RNA silencing encoded by a South Pacific isolate of Banana bunchy top virus originating from Pakistan

Identification of a major pathogenicity determinant and suppressors of RNA silencing encoded by a South Pacific isolate of Banana bunchy top virus originating from Pakistan

A Multifunctional Protein Encoded by Turkey Herpesvirus Suppresses RNA Silencing in Nicotiana benthamiana

Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

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