Identification of Tissue-Specific MicroRNAs from Mouse

Lagos-Quintana, Mariana; Rauhut, Reinhard; Yalçin, Ali; Meyer, Jutta; Lendeckel, Winfried; Tuschl, Thomas

doi:10.1016/s0960-9822(02)00809-6

Cited by 3,045 publications

(2,474 citation statements)

References 23 publications

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“…In the case of miR-375, expression of this miRNA has been reported in numerous tissue types including the pituitary gland, adrenal gland, intestine, lung, and several types of cancer [9] [10]. Similarly, miR-7 and miR-184 have been shown to be highly enriched in the central nervous system and eye, respectively [11]. Recent studies using mouse knockouts have shown that loss of these three abundant miRNAs will increase β-cell exocytosis however it still remains unclear whether all miRNAs present in the β-cell are committed to an identical functional role and whether they perform the same function in all cell types where they are present [12] [9] [8].…”

Section: Microrna Profiling In the Pancreatic β-Cell And Isletsmentioning

confidence: 99%

MicroRNAs: An adaptive mechanism in the pancreatic β-cell…and beyond?

Poy

2016

Best Practice & Research Clinical Endocrinology & Metab

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Recent protocols have been developed to differentiate human stem cells and fibroblasts into insulin-producing cells capable of releasing the hormone in a glucosestimulated manner. Limitations remain which prevent bringing these protocols to a clinical setting as these models must still undergo complete characterization. Advances in sequencing technologies have driven the identification of several noncoding RNA species including microRNAs (miRNAs). While their diversity and unique expression patterns across different tissues have made deciphering their precise functional role a significant challenge, studies using both cell lines and transgenic mouse models have made substantial progress in understanding their regulatory role on exocytosis and proliferation of the β-cell. These results also indicate miRNAs play an integral role in the fundamental mechanics of how the cell manages the balance between these independent functions. Continued investigation into miRNA function may uncover mechanisms which can be exploited to improve differentiation protocols in producing fully mature β-cells.

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Section: Microrna Profiling In the Pancreatic β-Cell And Isletsmentioning

confidence: 99%

MicroRNAs: An adaptive mechanism in the pancreatic β-cell…and beyond?

Poy

2016

Best Practice & Research Clinical Endocrinology & Metab

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“…5 The focus of the current study is miRNA 155 (miR-155), which is located on chromosome 21 and is transcribed from the B-cell integration cluster. 6 In addition to its involvement in proinflammatory responses, recent evidence indicates that this miRNA has a significant role in the process of carcinogenesis of several solid tumors, including colon cancer, 7 breast cancer, 8 and pancreatic ductal adenocarcinoma. 9 Recently, Tili et al reported that miR-155 can act as a mutator and, together with an miR-155-linked inflammatory environment, may be a potential mechanism connecting inflammation and cancer.…”

Section: Introductionmentioning

confidence: 99%

Aberrant expression of microRNA 155 may accelerate cell proliferation by targeting sex‐determining region Y box 6 in hepatocellular carcinoma

Xie

Chen

et al. 2011

Cancer

107

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BACKGROUND: Recent research has suggested that the oncomir microRNA 155 (miR-155) is up-regulated in hepatocellular carcinoma (HCC). In this study, the authors investigated the tumorigenic mechanism of this oncomir in the development of HCC. METHODS: Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was conducted to analyze the expressions of miR-155 and its potential target genes in paired tumor tissues and adjacent tumor-free tissues and in disease-free liver tissue samples. The in silico predicted target genes of miR-155 were assessed by dual-luciferase reporting assay, real-time RT-PCR, and Western blot analyses. U6 promoters that drive miR-155 precursor overexpression and miR-155 tough decoy knock-down constructs were used to study its affects on cell proliferation in vitro and on tumor formation in nude mice. RESULTS: Quantitative RT-PCR demonstrated a gradual ascension of miR-155 expression in cirrhotic liver tissues and in HCC tumor tissues compared with low expression levels in normal liver tissues. Ectopic expression of miR-155 in HepG2 cells enhanced its tumorigenesis, whereas depletion of the endogenous miR-155 reversed these tumorigenic properties. Ectopic expression of sex-determining region Y box 6 (SOX6) was able to reverse the growth-promoting property of miR-155. Concordantly, the results demonstrated for the first time that SOX6 is a direct target of miR-155. Further analysis revealed that SOX6 reduced cell growth by up-regulating p21waf1/cip1 expression in a p53-dependent manner. In addition, a decline in p21waf1/ cip1 expression caused by miR-155 could be reversed by SOX6 expression. CONCLUSIONS: The current data indicated that SOX6 is a novel target of miR-155 and that miR-155 enhances liver cell tumorigenesis at least in part through the novel miR-155/SOX6/p21waf1/cip1 axis. These findings suggest that miR-155 may be a potential target for HCC treatment.

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“…miRNAs also appear to have important roles in the mammalian central nervous system (CNS). Numerous miRNAs are expressed in the CNS [22][23][24], and many neural miRNAs are expressed in spatial and/or temporal patterns that suggest roles in the regulation of CNS development [25][26][27][28][29][30]. MiRNA miR-132 has been shown to regulate neuronal morphogenesis through decreasing levels of GTPase-activating protein, p250GAP [31] and (along with miR-219) has been implicated in the regulation of circadian rhythms [32].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA miR-124 regulates neurite outgrowth during neuronal differentiation

Chung

Deo

et al. 2008

Experimental Cell Research

290

211

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MicroRNAs (miRNAs) are small RNAs with diverse regulatory roles. The miR-124 miRNA is expressed in neurons in the developing and adult nervous system. Here we show that overexpression of miR-124 in differentiating mouse P19 cells promotes neurite outgrowth, while blocking miR-124 function delays neurite outgrowth and decreases acetylated α-tubulin. Altered neurite outgrowth also was observed in mouse primary cortical neurons when miR-124 expression was increased, or when miR-124 function was blocked. In uncommitted P19 cells, miR-124 expression led to disruption of actin filaments and stabilization of microtubules. Expression of miR-124 also decreased Cdc42 protein and affected the subcellular localization of Rac1, suggesting that miR-124 may act in part via alterations to members of the Rho GTPase family. Furthermore, constitutively active Cdc42 or Rac1 attenuated neurite outgrowth promoted by miR-124. To obtain a broader perspective, we identified mRNAs downregulated by miR-124 in P19 cells using microarrays. mRNAs for proteins involved in cytoskeletal regulation were enriched among mRNAs downregulated by miR-124. A miR-124 variant with an additional 5' base failed to promote neurite outgrowth and downregulated substantially different mRNAs. These results indicate that miR-124 contributes to the control of neurite outgrowth during neuronal differentiation, possibly by regulation of the cytoskeleton.

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Identification of Tissue-Specific MicroRNAs from Mouse

Cited by 3,045 publications

References 23 publications

MicroRNAs: An adaptive mechanism in the pancreatic β-cell…and beyond?

MicroRNAs: An adaptive mechanism in the pancreatic β-cell…and beyond?

Aberrant expression of microRNA 155 may accelerate cell proliferation by targeting sex‐determining region Y box 6 in hepatocellular carcinoma

MicroRNA miR-124 regulates neurite outgrowth during neuronal differentiation

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