Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica

Meng, Ying‐Ying; Cao, Xupeng; Yao, Changhong; Xue, Song; Yang, Qing

doi:10.1016/j.algal.2017.03.004

Cited by 40 publications

(34 citation statements)

References 42 publications

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“…Precursor ion and neutral loss scanning modes were employed to identify lipid species by the LipidSearch. For most lipid classes, fatty acid at the sn 1 or sn 2 position of the glycerol backbone was determined by following the published methods [30,46]. For some undetermined species, fatty acids at the sn -positions were estimated by comparing the relative intensities of the MS/MS fatty acid fragments to those of the characterized species of the same class under the same LC-MS/MS conditions.…”

Section: Methodsmentioning

confidence: 99%

“…However, the prediction is not always unambiguous. On the other hand, in algae and higher plants, glycerolipid synthesis pathways in ER (or the “eukaryotic” pathway) and plastid (or the “prokaryotic” pathway) can be distinguished by the fatty acids 18:X and 16:X at the sn 2 position of the glycerol backbone, respectively [27,28,29,30,31]. Hence, by combining the transcriptomic and lipidomic analyses, alteration of lipid classes under phosphorus limitation can be better revealed.…”

Section: Introductionmentioning

confidence: 99%

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Phosphorus-Induced Lipid Class Alteration Revealed by Lipidomic and Transcriptomic Profiling in Oleaginous Microalga Nannochloropsis sp. PJ12

et al. 2019

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Phytoplankton are primary producers in the marine ecosystem, where phosphorus is often a limiting factor of their growth. Hence, they have evolved strategies to recycle phosphorus by replacing membrane phospholipids with phosphorus-free lipids. However, mechanisms for replacement of lipid classes remain poorly understood. To improve our understanding, we performed the lipidomic and transcriptomic profiling analyses of an oleaginous marine microalga Nannochloropsis sp. PJ12 in response to phosphorus depletion (PD) and replenishing. In this study, by using (liquid chromatography couple with tandem mass spectrometry) LC-MS/MS-based lipidomic analysis, we show that membrane phospholipid levels are significantly reduced upon PD, while phosphorus-free betaine lipid levels are increased. However, levels of phosphorus-free photosynthetic galactolipid and sulfolipid are not increased upon PD, consistent with the reduced photosynthetic activity. RNA-seq-based transcriptomic analysis indicates that enzymes involved in phospholipid recycling and phosphorus-free lipid synthesis are upregulated, supporting the lipidomic analysis. Furthermore, enzymes involved in FASII (type II fatty acid synthesis) elongation cycle upon PD are transcriptionally downregulated. EPA (eicosapentaenoic acid) level decrease upon PD is revealed by both GC-MS (gas chromatography coupled with mass spectrometry) and LC-MS/MS-based lipidomic analyses. PD-induced alteration is reversed after phosphorus replenishing. Taken together, our results suggest that the alteration of lipid classes upon environmental change of phosphorus is a result of remodeling rather than de novo synthesis in Nannochloropsis sp. PJ12.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phosphorus-Induced Lipid Class Alteration Revealed by Lipidomic and Transcriptomic Profiling in Oleaginous Microalga Nannochloropsis sp. PJ12

et al. 2019

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“…The combined organic phases were dried by N 2 flow. The dried residue was re-dissolved in 1 mL of chloroform (Meng et al 2017).…”

Section: Methodsmentioning

confidence: 99%

“…After transesterification, 0.75 mL de-ionized water and 2 ml n -hexane were added to the vial and the top n -hexane layer was removed and placed into vials with a small amount of sodium sulphate for GC analysis. Fatty-acid methyl esters (FAMEs) analyses were carried out by an Agilent 6890 GC instrument was equipped with a flame-ionization detector (FID) and a DB-23 capillary column (Agilent Technologies, USA, 30 m × 0.32 mm × 0.25 µm) (Meng et al 2017).…”

Section: Methodsmentioning

confidence: 99%

The heat-tolerance evaluation of an Isochrysis zhangjiangensis mutant generated by atmospheric and room temperature plasmas

et al. 2019

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Isochrysis zhangjiangensis is widely used in the marine aquaculture as larval feed, especially for filter feeding cultures, as well as a good candidate for biofuels. However, the optimal cultivation temperature for I. zhangjiangensis is below 30 °C and this stain is seriously affected by high temperature, which causes the limited application during the summer. I. zhangjiangensis IM130005 is a strain generated by atmospheric and room temperature plasmas with relative higher growth rate and lipid production than the wide strain (WT), with the ability to tolerate several hours’ high temperature during the outdoor cultivation. Here, a detailed comparison was performed by continuous monitoring growth, chlorophyll fluorescence and fatty acid profile between IM13005 and WT under a mimic temperature shock to the summer outdoor cultivation. Based on a nearly 20% increase of total fatty acid in IM13005, which was majorly contributed by saturated or monounsaturated FAs in form of neutral lipids, within 5 h under the heat shock, the fatty acids and lipids synthesis variation were postulated as the physiological reason for the high temperature tolerance.

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“…oceanica proposed the role of phosphatidylcholine for TAG biosynthesis and carbon‐chain length improvement under nitrogen starvation as it converts C 16 acyls into C 18 TAG. They also suggested that diacylglyceryl‐ N ‐trimethylhomoserine and phosphatidylethanolamine are the precursor of eicosatetraenoic acid and other lipids . Lipidomic analysis of four Botryococcus strains revealed the production of very‐long FA, which constituted up to 1% of total FA content of these strains.…”

Section: Identification Of Genetic Engineering Targetsmentioning

confidence: 99%

Engineering the metabolic pathways of lipid biosynthesis to develop robust microalgal strains for biodiesel production

Shahid

Rehman

Usman

et al. 2019

Biotech and App Biochem

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Algal lipids have shown promising feedstock to produce biodiesel due to higher energy content, higher cetane number, and renewable nature. However, at present, the lipid productivity is too low to meet the commercial needs. Various approaches can be employed to enhance the lipid content and lipid productivity in microalgae. Stress manipulation is an attractive option to modify the algal lipid content, but it faces the drawback of time‐consuming production processing and lack of information about molecular mechanisms related to triacylglycerides production in response to stress. Developing the robust hyper lipid accumulating algal strains has gained momentum due to advances in metabolic engineering and synthetic biology tools. Understanding the molecular basis of lipid biosynthesis followed by reorienting the related pathways through genomic modification is an alluring strategy that is believed to achieve the industrial and economic robustness. This review portrays the use of integrated OMIC approaches to elucidate the molecular mechanisms of strain adaptability in response to stress conditions, and identification of molecular pathways that should become novel targets to develop novel algal strains. Moreover, an update on the metabolic engineering approaches to improve the lipid production in microalgae is also provided.

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Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica

Cited by 40 publications

References 42 publications

Phosphorus-Induced Lipid Class Alteration Revealed by Lipidomic and Transcriptomic Profiling in Oleaginous Microalga Nannochloropsis sp. PJ12

Phosphorus-Induced Lipid Class Alteration Revealed by Lipidomic and Transcriptomic Profiling in Oleaginous Microalga Nannochloropsis sp. PJ12

The heat-tolerance evaluation of an Isochrysis zhangjiangensis mutant generated by atmospheric and room temperature plasmas

Engineering the metabolic pathways of lipid biosynthesis to develop robust microalgal strains for biodiesel production

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