Identification of the iron-limitation stimulon in Staphylococcus lugdunensis

Aubourg, Marion; Gravey, François; Dhalluin, Anne; Giard, Jean‐Christophe

doi:10.1007/s00203-021-02342-2

Cited by 3 publications

(3 citation statements)

References 37 publications

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“… S. lugdunensis is particularly well equipped with iron-metabolism enzymes playing an important role in the “nutritional immunity” setting up by the host to limit access to essential ions. Interestingly, we identified several AgrA upregulated genes known to be involved in iron homeostasis ( isdB , isdE , efeB ) and hemin detoxification (SLUG_06770, SLUG_06780) ( 17 ).…”

Section: Resultsmentioning

confidence: 99%

“…It is probably the most aggressive CoNS species and several virulence factors have been characterized ( 15 ). Likewise, S. lugdunensis is well-equipped for the acquisition of nutritional iron, which has been shown to be crucial for biofilm formation, virulence, and resistance to oxidative stress ( 16 , 17 ). This a priori harmless commensal CoNS should therefore be regarded as an opportunistic pathogen.…”

Section: Introductionmentioning

confidence: 99%

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Inactivation of the Response Regulator AgrA Has a Pleiotropic Effect on Biofilm Formation, Pathogenesis and Stress Response in Staphylococcus lugdunensis

et al. 2022

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Although belonging to the natural human skin flora, Staphylococcus lugdunensis is recognized as a particularly aggressive and destructive pathogen. This study aimed to characterize the role of the response regulator AgrA, which is a component of the quorum-sensing agr system and known to be a major element in the regulation of pathogenicity and biofilm formation in Staphylococcus aureus .

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inactivation of the Response Regulator AgrA Has a Pleiotropic Effect on Biofilm Formation, Pathogenesis and Stress Response in Staphylococcus lugdunensis

et al. 2022

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“…S. lugdunensis strains have been proven to be hemolytic toward human erythrocytes but not toward murine erythrocytes [43]. There is converging evidence that agrA is a strong positive regulator of the expression of slush hemolysins [29,44]. The lack of hemolytic activity on murine erythrocytes would suggest overt human tropism for S. lugdunensis.…”

Section: Discussionmentioning

confidence: 99%

Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity

Ravaioli,

Campoccia,

Mirzaei

et al. 2023

IJMS

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Staphylococcus lugdunensis is an emerging high-virulent pathogen. Here, the presence and expression of virulence genes (icaA, fbl, vwbl, fbpA, slush A, B and C, and genes of the putative β-hemolysin and hemolysin III) and the ability to induce synergistic hemolytic activity and hemolysis after 24, 48 and 72 h were investigated in a collection of twenty-two S. lugdunensis clinical isolates. The collection of isolates, mainly from implant orthopedic infections, had previously been grouped by ribotyping/dendrogram analysis and studied for biofilm matrices, biomasses and antibiotic resistances. Two isolates, constituting a unique small ribogroup sharing the same cluster, exhibited an amplicon size of the slush operon (S. lugdunensis synergistic hemolysin) which was shorter than the expected 977 bp. This outcome can predict the genetic lineage of the S. lugdunensis strains. One isolate (cra1342) presented two deletions: one of 90 bp in slush A and the other of 91 bp in slush B. Another isolate (N860314) showed a single 193 bp deletion, which encompassed part of the slush B terminal sequence and most of slush C. The isolate N860314 was devoid of hemolytic activity after 24 h, and the first consideration was that the deleted region deals with the coding of the active enzymatic site of the slush hemolysin. On the other hand, cra1342 and N860314 isolates with different slush deletions and with hemolytic activity after 24 and 48 h, respectively, could have replaced the hemolytic phenotype through other processes.

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