Identification of the constitutive ultradian oscillator of the circadian clock (ENOX1) in &amp;lt;i&amp;gt;Saccharomyces cerevisiae&amp;lt;/i&amp;gt;

Dick, Sara; Ryuzoji, Aya; Morré, Dorothy M.; Morré, D. James

doi:10.4236/abc.2013.33036

Cited by 7 publications

(14 citation statements)

References 36 publications

(46 reference statements)

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“…As reported previously [16], the yeast Saccharomyces cerevisiae exhibits an ENOX1 activity with a period length of 24 min similar to that of other eucaryotes [15]. However, S. cerevisiae has a second ENOX1-like activity with a period length of 25 min [16].…”

Section: Discussionsupporting

confidence: 73%

“…It contained a S219LGPFG putative adenine binding motif and putative copper binding motifs Y246PDH and Y335LYas well as a potential protein disulfide interchange motif C23KIVFSC. There were essentially no homologies between the amino acid sequence of the YDR005C and the yeast ENOX1 protein YM117W with the 24 min period length [16].…”

Section: Resultsmentioning

confidence: 98%

“…However, S. cerevisiae has a second ENOX1-like activity with a period length of 25 min [16]. The latter is different from the yeast ENOX1 and ENOX1 proteins in general not only from the longer period length but also by being resistant to the specific ENOX1 inhibitor simalikalactone D and failure of the 25 min rhythm to be phased by melatonin.…”

Section: Discussionmentioning

confidence: 93%

“…This allows each dimer to transfer four electrons and produce two molecules of water from one oxygen molecule. Both the mammalian ENOX1 [20] and the yeast ENOX1 [16] contain two or more copper binding sites which, when mutated, lead to a loss of ENOX1 activity.…”

Section: Discussionmentioning

confidence: 99%

“…Measurements of enzymatic activities were as described previously [16]. The oxidation of NADH was measured from the disappearance of NADH at 340 nm.…”

Section: Measurements Of Enzymatic Activitiesmentioning

confidence: 99%

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Molecular Cloning and Characterization of a Candidate ENOX Protein of <i>Saccharomyces cerevisiae</i> with a 25 Min Period Insensitive to Simalikalactone D Inhibition and Melatonin

Ryuzoji¹,

Parisi²,

Dick³

et al. 2014

ABC

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A yeast deletion library was screened based on NADH fluorescence using a 384-well plate assay to identify a yeast isolate lacking a previously identified cell surface oxidase exhibiting an oscillatory pattern with a period length of 25 min and resistant to the ENOX1-specific inhibitor simalikalactone D (YNOX for yeast-specific ENOX = ENOX4). The cDNA was cloned from a yeast over expression library using NADH fluorescence analyzed by Fast Fourier transform and decomposition fits. The objective was to identify and sequence an ENOX homologue in Saccharomyces cerevisiae with a 25 min rather than a 24 min period length (YNOX). The finding identified YDR005C as the yeast ENOX protein with a temperature-independent 25 min period length and insensitive to inhibition by simalikalactone D. The encoded protein was expressed in bacteria and characterized. Gel slices corresponding to 55 kDa and 39 kDa His-tagged proteins exhibited 25 min oscillatory patterns not inhibited by 1 µM simalikalactone D for both NADH oxidation and reduced coenzyme Q10 oxidation as well as a protein disulfide-thiol interchange activity which alternated with the oxidative activities. Activities were phased by low-frequency electromagnetic fields but, in contrast, to yeast ENOX1, not by addition of melatonin. The assay in the presence of D 2 O shifted the length of the oscillatory period from 25 min to 32 min. The YDR005C deletion mutant cells lacked the ENOX4 clock output present in the wild type yeast.

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Section: Discussionsupporting

confidence: 73%

Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

“…Measurements of enzymatic activities were as described previously [16]. The oxidation of NADH was measured from the disappearance of NADH at 340 nm.…”

Section: Measurements Of Enzymatic Activitiesmentioning

confidence: 99%

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Molecular Cloning and Characterization of a Candidate ENOX Protein of <i>Saccharomyces cerevisiae</i> with a 25 Min Period Insensitive to Simalikalactone D Inhibition and Melatonin

Ryuzoji¹,

Parisi²,

Dick³

et al. 2014

ABC

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Widespread patterns of sexually dimorphic gene expression in an avian hypothalamic–pituitary–gonadal (HPG) axis

MacManes

Austin

Lang

et al. 2017

Sci Rep

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The hypothalamic-pituitary-gonadal (HPG) axis is a key biological system required for reproduction and associated sexual behaviors to occur. In the avian reproductive model of the rock dove (Columba livia), we characterized the transcript community of each tissue of the HPG axis in both sexes, thereby significantly expanding our mechanistic insight into HPG activity. We report greater sex-biased differential expression in the pituitary as compared to the hypothalamus, with multiple genes more highly expressed in the male pituitary being related to secretory function, and multiple genes more highly expressed in the female pituitary being related to reproduction, growth, and development. We report tissue-specific and sex-biased expression in genes commonly investigated when studying reproduction, highlighting the need for sex parity in future studies. In addition, we uncover new targets of investigation in both sexes, which could potentially change our understanding of HPG function.

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Widespread patterns of sexually dimorphic gene expression in an avian hypothalamic–pituitary–gonadal (HPG) axis

MacManes

Austin

Lang

et al. 2016

Preprint

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35The hypothalamic-pituitary-gonadal (HPG) axis is a key biological system required for 36 reproduction and associated sexual behaviors to occur. In the avian reproductive model of the 37 rock dove (Columba livia), we characterized the transcript community of each tissue of the HPG 38 axis in both sexes, thereby significantly expanding our mechanistic insight into HPG activity. We 39 report greater sex-biased differential expression in the pituitary as compared to the 40 hypothalamus, with multiple genes more highly expressed in the male pituitary being related to 41 secretory function, and multiple genes more highly expressed in the female pituitary being 42 related to reproduction, growth, and development. We report tissue-specific and sex-biased 43 expression in genes commonly investigated when studying reproduction, highlighting the need 44 for sex parity in future studies. In addition, we uncover new targets of investigation in both 45 sexes, which could potentially change our understanding of HPG function. 46 47 INTRODUCTION 48

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Identification of the constitutive ultradian oscillator of the circadian clock (ENOX1) in <i>Saccharomyces cerevisiae</i>

Cited by 7 publications

References 36 publications

Molecular Cloning and Characterization of a Candidate ENOX Protein of <i>Saccharomyces cerevisiae</i> with a 25 Min Period Insensitive to Simalikalactone D Inhibition and Melatonin

Molecular Cloning and Characterization of a Candidate ENOX Protein of <i>Saccharomyces cerevisiae</i> with a 25 Min Period Insensitive to Simalikalactone D Inhibition and Melatonin

Widespread patterns of sexually dimorphic gene expression in an avian hypothalamic–pituitary–gonadal (HPG) axis

Widespread patterns of sexually dimorphic gene expression in an avian hypothalamic–pituitary–gonadal (HPG) axis

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Identification of the constitutive ultradian oscillator of the circadian clock (ENOX1) in &lt;i&gt;Saccharomyces cerevisiae&lt;/i&gt;

Cited by 7 publications

References 36 publications

Molecular Cloning and Characterization of a Candidate ENOX Protein of &lt;i&gt;Saccharomyces cerevisiae&lt;/i&gt; with a 25 Min Period Insensitive to Simalikalactone D Inhibition and Melatonin

Molecular Cloning and Characterization of a Candidate ENOX Protein of &lt;i&gt;Saccharomyces cerevisiae&lt;/i&gt; with a 25 Min Period Insensitive to Simalikalactone D Inhibition and Melatonin

Widespread patterns of sexually dimorphic gene expression in an avian hypothalamic–pituitary–gonadal (HPG) axis

Widespread patterns of sexually dimorphic gene expression in an avian hypothalamic–pituitary–gonadal (HPG) axis

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Identification of the constitutive ultradian oscillator of the circadian clock (ENOX1) in <i>Saccharomyces cerevisiae</i>

Molecular Cloning and Characterization of a Candidate ENOX Protein of <i>Saccharomyces cerevisiae</i> with a 25 Min Period Insensitive to Simalikalactone D Inhibition and Melatonin

Molecular Cloning and Characterization of a Candidate ENOX Protein of <i>Saccharomyces cerevisiae</i> with a 25 Min Period Insensitive to Simalikalactone D Inhibition and Melatonin