Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck

Abstract

White Pekin duck is an important meat resource in the livestock industries. However, the temperature increase due to global warming has become a serious environmental factor in duck production, because of hyperthermia. Therefore, identifying the gene regulations and understanding the molecular mechanism for adaptation to the warmer environment will provide insightful information on the acclimation system of ducks. This study examined transcriptomic responses to heat stress treatments (3 and 6 h at 35 °C) and control (C, 25 °C) using RNA-sequencing analysis of genes from the breast muscle tissue. Based on three distinct differentially expressed gene (DEG) sets (3H/C, 6H/C, and 6H/3H), the expression patterns of significant DEGs (absolute log2 > 1.0 and false discovery rate < 0.05) were clustered into three responsive gene groups divided into upregulated and downregulated genes. Next, we analyzed the clusters that showed relatively higher expression levels in 3H/C and lower levels in 6H/C with much lower or opposite levels in 6H/3H; we referred to these clusters as the adaptable responsive gene group. These genes were significantly enriched in the ErbB signaling pathway, neuroactive ligand-receptor interaction and type II diabetes mellitus in the KEGG pathways (P < 0.01). From the functional enrichment analysis and significantly regulated genes observed in the enriched pathways, we think that the adaptable responsive genes are responsible for the acclimation mechanism of ducks and suggest that the regulation of phosphoinositide 3-kinase genes including PIK3R6, PIK3R5, and PIK3C2B has an important relationship with the mechanisms of adaptation to heat stress in ducks.

Comparative transcriptome analysis of Ethiopian indigenous chickens from low and high altitudes under heat stress condition reveals differential immune response

“…The ErbB signaling pathway has also been found to be associated with heat stress in duck (Kim et al . ). Glycerophospholipid metabolism, a lipid metabolism pathway, has an important role in maintaining energy homeostasis in thermoregulatory responses and is a fundamental biological process in the immune system under heat stress (Renaudeau et al .…”

Analysis of circulating-microRNA expression in lactating Holstein cows under summer heat stress

“…In mammalian cells, the cytoskeleton, which is susceptible to heat shock, can degrade or dysfunction under HS conditions, resulting in changes in mitochondria distribution, leading to reduced ATP production [48–50]. Rap1 signaling pathway (bta04015) was found to be closely associated with energy balance control, including glucose homeostasis and lipid metabolism, and it has been reported to play an important role in regulating energy homeostasis in HS conditions [51, 52]. In a previous study, Kim et al inferred that genes involved in these pathways induce energy homeostasis in response to relatively high energy demands under HS conditions [51].…”

“…Rap1 signaling pathway (bta04015) was found to be closely associated with energy balance control, including glucose homeostasis and lipid metabolism, and it has been reported to play an important role in regulating energy homeostasis in HS conditions [51, 52]. In a previous study, Kim et al inferred that genes involved in these pathways induce energy homeostasis in response to relatively high energy demands under HS conditions [51]. We also found rapamycin (mTOR) signaling pathway (bta04150), which plays a vital role in several cellular functions such as glucose and lipid metabolism(homeostasis), cellular growth, proliferation, survival, aging, and actin cytoskeletal regulation [53].…”

Liver Transcriptome Changes of Hyla Rabbit in Response to Chronic Heat Stress

“…Heat stress could lead to enormous economic loss in rabbit industry by decreasing feed intake and increasing mortality rate [19,20]. Transcriptome studies have been widely used to explore the underlying molecular basis of heat resistance in animals, such as the cow [2], sheep [8], chicken [3], and duck [6]. In the current study, we artificially induced heat stress condition in rabbit, by which the global gene expression profiles have been studied.…”

“…Generally, R 2 > 0.8 was considered suitable. For exploring gene expression profile in each sample, all the genes were categorized into five groups based on their FPKM values: very low expression gene (0-1), low expression gene (1-3), medium expression gene (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15), high expression gene (15-60), and extremely high expression gene (>60).…”