Identification of telocytes in dystrophic mice testis

Gomes, Vilessa Lilian de Araújo; Braz, Janine Karla França da Silva; Martins, Gabriel Moura; Clebis, Naianne Kelly; Oliveira, Moacir Franco de; Morais, Danielle Barbosa; Moura, Carlos Eduardo Bezerra de

doi:10.31744/einstein_journal/2021ai5737

Cited by 4 publications

(4 citation statements)

References 7 publications

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“…Without overstating, it can be said that TCs have been described in almost every organ one can think of. Except of the FRS, TCs have been discovered in the intestine [18], lungs [19], skeletal muscle [20], skin [21], ureter [22], heart [23], testes [24], prostate [25], eye [26], and even in remarkable locations such as a cow s teat [27]. TCs in the FRS are quite alike to TCs in other organs, but there are several morphological and functional characteristics that make them distinct.…”

Section: Morphological and Functional Specifics Of Frs Telocytesmentioning

confidence: 99%

Telocytes in the Female Reproductive System: Up-to-Date Knowledge, Challenges and Possible Clinical Applications

Klein

Csöbönyeiová

Danišovič

et al. 2022

Life

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From their initial description in 2005 to this day, telocytes (TCs) have been described in the ovary, uterine tubes, uterus, vagina, mammary gland, and placenta. Their morphological features, immunophenotype, physiological functions, and roles in disease have been thoroughly documented in both animal models and human subjects. TCs, with their extremely long cytoplasmic processes called telopodes, play a pivotal role in the morphological and functional interconnection of all the components of the interstitial compartment, but also with constituents of the parenchyma. Although there is no specific immunohistochemical marker for their identification, the most cited are CD 117, CD 34, platelet-derived growth factor receptor (PDGFR), vimentin, and specific markers typical for the female reproductive system (FRS)—estrogen and progesterone receptors (ER and PR). This immunophenotype provides important clues to their physiological roles. Their main functions include the regulation of hormone-dependent processes, intercellular signaling, immune surveillance, microenvironmental maintenance, and the nursing of stem cells. In a situation where TCs are functionally or morphologically decimated, many disease entities may develop, including premature ovarian failure, endometriosis, ectopic pregnancy, infertility, preeclampsia, or even breast cancer. The common denominator of many of these conditions is that their etiopathogenesis is either partially known or completely obscure. Even though the exact role of TCs in these conditions is yet to be revealed, multiple lines of research indicate that their future clinical application may enrich diagnostic-therapeutic strategies of countless conditions. TCs are also heavily debated in terms of their possible use in regenerative medicine and tissue engineering. Some of the concepts related to TC research are strongly substantiated by experimental data, while others are highly speculative. Only future research endeavors will clearly distinguish dead-end lines of research from genuine contributions to the field.

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Section: Morphological and Functional Specifics Of Frs Telocytesmentioning

confidence: 99%

Telocytes in the Female Reproductive System: Up-to-Date Knowledge, Challenges and Possible Clinical Applications

Klein

Csöbönyeiová

Danišovič

et al. 2022

Life

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“…Furthermore, telocytes were evidenced in both control and dystrophic group testes. Telocytes are a new type of classic interstitial cells first described in the early 21 st century (Hinescu and Popescu, 2005) present in several organs, including the human testicle (Marini et al, 2018) and mdx mice testes (Gomes et al, 2021). Although their function has not yet been elucidated, some hypotheses indicate that these cells may be involved in the synthesis of testis lipids in the genital system (Sanches et al, 2021), is due to the communication of cytoplasmic processes through telopods (Tp), with multilamellar bodies (lipid storage) located in the Leydig cell cytoplasm (Haseeb et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Although their function has not yet been elucidated, some hypotheses indicate that these cells may be involved in the synthesis of testis lipids in the genital system (Sanches et al, 2021), is due to the communication of cytoplasmic processes through telopods (Tp), with multilamellar bodies (lipid storage) located in the Leydig cell cytoplasm (Haseeb et al, 2019). Furthermore, the presence of testicular telocytes in dystrophic mice seems to imply in spermatogenesis mechanisms, potentially aiding in seminiferous tubuletestosterone uptake (Gomes et al, 2021). Thus, it is possible that these cells may play a role in the testis of dystrophic mice, although further investigations on this specific topic are required.…”

Section: Discussionmentioning

confidence: 99%

Intertubular morphometric and ultrastructural testes analyses in mdx mice

et al. 2022

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Duchenne Muscular Dystrophy (DMD) reproductive alterations and the influence of antioxidant treatments may aid in understanding morphometry testicular quantification. In this context, the aim of the present study was to characterize the intertubular compartment (ITC) morphometry of animal testes in mdx mice supplemented with ascorbic acid (AA). Sixteen mice were used, namely the C57BL/10 (non-dystrophic) and C57BL/10Mdx (dystrophic) lineages, distributed into the following groups: Control (C60), Dystrophic (D60), Control supplemented with AA (CS60), Dystrophic supplemented with AA (DS60). A total of 200 mg/kg of AA were administered to mice for 30 days. Subsequently, the testicles were collected, weighed, and fragmented. The obtained fragments were fixed in Karnovsky's solution (pH 7.2) and embedded in historesin for morphometric and transmission electron microscopy assessments. Leydig cells were hypertrophic in the D60 group, but was reverted by AA supplementation in the DS60 group. The DS60 group also exhibited increased intertubular volume compared to the CS60 group. The ultrastructural images identified multilamellar bodies in dystrophic animals (lipid storage) and telocyte cells (transport substances) in both control and dystrophic animals. Morphometric alterations were, therefore, noted in the intertubular compartment due to Duchenne muscular dystrophy (DMD), with AA administration capable of altering Leydig cells in this condition.

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“…Telocytes are also found in all of the heart's layers, including the epicardium, the endocardium and the myocardium. Telocytes are found throughout the reproductive system, including the prostate, testesuterus, myometrium and placenta [4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Uterine Rat Telocyte Structure and Organization: An Immunohistochemical and Ultrastructural Study

ES¹

2022

CHIJ

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Telocytes are a kind of interstitial cell found in a variety of organs, including the uterus. They regulate uterine contractions, maintain pregnancy, and prevent premature labour. The purpose of this research was to look at the organisation and distribution of telocytes in different uterine layers in adult and senile rats at various stages of reproduction. Materials & Methods: Twenty-four female rats were evenly divided between four groups: GI: adult non-pregnant, GII pregnant, GIII postpartum, and GIV senile. The uterine samples from middle one-third of the right horns were processed for light microscopic examination were treated using C-kit stains for immunohistochemical detection of telocytes, and TEM examination. Results: Telocytes were discovered in the endometrium and myometrium of adult non-pregnant uteri as tiny cells with numerous lengthy telopodes. Endometrial telocytes increased significantly, but myometrial telocytes decreased significantly in (GII). (GIII) and (GIV) had the largest count of myometrial telocytes. Conclusion: Telocytes were found in the endometrium and myometrium of the rat uterus. Telocytes serve as a uterine peacekeeper, initiating and coordinating myometrial contraction.

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Identification of telocytes in dystrophic mice testis

Cited by 4 publications

References 7 publications

Telocytes in the Female Reproductive System: Up-to-Date Knowledge, Challenges and Possible Clinical Applications

Telocytes in the Female Reproductive System: Up-to-Date Knowledge, Challenges and Possible Clinical Applications

Intertubular morphometric and ultrastructural testes analyses in mdx mice

Uterine Rat Telocyte Structure and Organization: An Immunohistochemical and Ultrastructural Study

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