Identification of Skin Injury-related Genes Induced by Ionizing Radiation in Human Keratinocytes using cDNA Microarray

Koike, Manabu; Shiomi, Tomoe; Koike, Aki

doi:10.1269/jrr.46.173

Cited by 32 publications

(39 citation statements)

References 33 publications

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“…Interestingly, several apoptosis-related genes were found to be up-regulated in NHEK cells after a 20 Gy exposure to g-rays [15]. Similarly, reduction in antioxidants defences, i.e.…”

Section: Introductionmentioning

confidence: 98%

Human keratinocyte radiosensitivity is linked to redox modulation

Isoir

Buard

Gasser³

et al. 2006

Journal of Dermatological Science

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“…Interestingly, several apoptosis-related genes were found to be up-regulated in NHEK cells after a 20 Gy exposure to g-rays [15]. Similarly, reduction in antioxidants defences, i.e.…”

Section: Introductionmentioning

confidence: 98%

Human keratinocyte radiosensitivity is linked to redox modulation

Isoir

Buard

Gasser³

et al. 2006

Journal of Dermatological Science

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“…Interestingly, when keratinocytes were exposed to gamma irradiation or x-rays, the transcriptional changes were similar to those found in the UV-treated cells (Koike, 2005;Lamartine, 2005). Specifically, the genes involved in cell energy metabolism appeared to be particularly responsive.…”

Section: Effects Of Uv and Other Environmental Stressmentioning

confidence: 62%

DNA Microarrays in Dermatology and Skin Biology

Blumenberg

2006

OMICS: A Journal of Integrative Biology

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Because of its accessibility, skin has been among the first organs analyzed using DNA microarrays. Skin cancers, melanomas, and basal and squamous cell carcinomas have been intensely investigated because they are very frequent and can be fatal. Psoriasis, one of the most common human inflammatory diseases, has been studied comprehensively using DNA microarrays. In addition, epidermal keratinocytes have been the target of many studies because they respond to a rich variety of inflammatory and immunomodulating cytokines, hormones, vitamins, ultraviolet (UV) light, toxins, and physical injury. Because of the ethical considerations, the effects of harmful or dangerous agents on skin have been studied using artificial skin substitutes. Transcriptional mechanisms that regulate epidermal differentiation and cornification have begun to yield their mysteries, and very exciting recent studies identified the genes specifically expressed in epidermal stem cells. Thus, skin has everything: stem cells, differentiation, signaling, inflammation, diseases, and cancer. All these exciting facets of skin have been explored using DNA microarrays. Researchers in skin biology and dermatology were among the first to implement this technology and we expect that they will continue to generate exciting and useful new knowledge.

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“…The large volume of data provided by microarrays allows researchers to demonstrate individual preferences, interests and inclinations. In keratinocytes exposed to -irradiation or X-rays, the transcriptional changes were similar to those in the UV-treated cells; specifically, the genes involved in energy metabolism were induced [49,50].…”

Section: Studies Focused On Uv Lightmentioning

confidence: 70%

SKINOMICS: Transcriptional Profiling in Dermatology and Skin Biology

Blumenberg¹

2015

Encyclopedia of Metagenomics

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Recent years witnessed the birth of bioinformatics technologies, which greatly advanced biological research. These 'omics' technologies address comprehensively the entire genome, transcriptome, proteome, microbiome etc. A large impetus in development of bioinformatics was the introduction of DNA microarrays for transcriptional profiling. Because of its accessibility, skin was among the first organs analyzed using DNA microarrays, and dermatology among the first medical disciplines to embrace the approach. Here, DNA microarray methodologies and their application in dermatology and skin biology are reviewed. The most studied disease has been, unsurprisingly, melanoma; markers of melanoma progression, metastatic potential and even melanoma markers in blood have been detected. The basal and squamous cell carcinomas have also been intensely studied. Psoriasis has been comprehensively explored using DNA microarrays, transcriptional changes correlated with genomic markers and several signaling pathways important in psoriasis have been identified. Atopic dermatitis, wound healing, keloids etc. have been analyzed using microarrays. Noninvasive skin sampling for microarray studies has been developed. Simultaneously, epidermal keratinocytes have been the subject of many skin biology studies because they respond to a rich variety of inflammatory and immunomodulating cytokines, hormones, vitamins, UV light, toxins and physical injury. The transcriptional changes occurring during epidermal differentiation and cornification have been identified and characterized. Recent studies identified the genes specifically expressed in human epidermal stem cells. As dermatology advances toward personalized medicine, microarrays and related 'omics' techniques will be directly applicable to the personalized dermatology practice of the future.

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Identification of Skin Injury-related Genes Induced by Ionizing Radiation in Human Keratinocytes using cDNA Microarray

Cited by 32 publications

References 33 publications

Human keratinocyte radiosensitivity is linked to redox modulation

Human keratinocyte radiosensitivity is linked to redox modulation

DNA Microarrays in Dermatology and Skin Biology

SKINOMICS: Transcriptional Profiling in Dermatology and Skin Biology

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