Identification of Single Nucleotide Polymorphisms (SNPs) of the Bovine Growth Hormone (bGH) Gene Associated with Growth and Carcass Traits in Hanwoo

Abstract

The purpose of this study was to find any association of the bovine growth hormone (bGH) gene with growth and carcass quality traits in Korean native cattle, Hanwoo. Genomic DNA was extracted from 21 Hanwoo individuals, and the 47 to 2,528 bp region of the bGH 2,856 bp (GenBank accession number M57764) including the promoter and the five exons was sequenced. A total of ten bGH SNPs were confirmed, including four (253 C>T, 303 C>T, 502 C>T, and 559 G>A) in the promoter, one (679 C>T) in exon 1, one (1,692 T>C) in intron 3, and four (2141 C>G, 2258 C>T, 2277 C>T, and 2291 A>C) in exon 5. The ten bGH SNPs were genotyped for a sample of 242 Hanwoo steers and association tests were performed to find any significant SNP that was correlated with growth and carcass quality. Of the SNPs, the 303 C>T SNP in the promoter region was significantly associated with 6-month-old weight, the 559 G>A SNP with longissimus dorsi muscle area, the 2141 C>G SNP in exon 5 with daily weight gain, and the 2258 C>T SNP with daily weight gain and carcass weight (p<0.05). The significant SNPs need to be verified in other Hanwoo populations before considering implementation of marker-assisted selection for genetic improvement of growth and carcass quality in Hanwoo.

Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene

“…Dybus (2002) also confirmed that SNP g.1047C>T was associated with milk quality in Polish Black-and-White cattle. In addition, Lee et al (2013) reported that the SNP g.1642C>G was associated with daily weight gain in the Hanwoo (Bos taurus) cattle with moderate PIC value. In contrast, Agung et al (2017) reported that SNP g.1047C>T was not associated with birth weight, weaning weight, yearling weight, and dressing percentage in the SO cattle.…”

Yerli Sudan Baggara Sığırında Büyüme Hormonu ve İnsülin Benzeri Büyüme Faktörü 1 Genlerinin Seçili Genetik Polimorfizmlerinin Moleküler Tespiti

“…A similar result was mentioned by another group in Indonesia [18] who reported the same C>T SNP as a new mutation in The second mutation (2258 bp C>T) appeared in the two strains of Baggara cattle. The 2258 C>T SNP was previously associated with average daily weight gain (ADG) and carcass weight (CWT) [19] . The high allele frequency of the favorable allele (C) in both European and Asian Bos taurus breeds indicated that selection for genetic improvement of ADG and CWT is associated with the SNP or a locus at Linkage disequilibrium with the SNP [19] .…”

“…The 2258 C>T SNP was previously associated with average daily weight gain (ADG) and carcass weight (CWT) [19] . The high allele frequency of the favorable allele (C) in both European and Asian Bos taurus breeds indicated that selection for genetic improvement of ADG and CWT is associated with the SNP or a locus at Linkage disequilibrium with the SNP [19] . There is a need to further investigate this important mutation in all strains of Baggara cattle.…”

The effect of SNP polymorphisms in growth hormone gene on weight and linear growth in crossbred Red Angus × Kalmyk heifers

“…The AluI polymorphism is associated with the replacement of bases C-G in the 5th exon of the bGH gene, as a result the amino acid Leu is replaced with the amino acid Val (L127V, rs 4192384). There is an association of various polymorphic variants of the bGH gene with different productive traits in cattle [13].…”