Identification of Single Nucleotide Polymorphism and Association Analysis of Alpha 2-Heremans Schmid Glycoprotein (AHSG) Gene Related to Fatty Acid Traits in Sheep

Munyaneza, Jean Pierre; Gunawan, Asep; Noor, Ronny Rachman

doi:10.32628/ijsrst196176

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…The polymorphism in OLFML3 gene was significantly associated with FAs composition including palmitoleic acid (C16:1), heptadecanoic acid (C17:0), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), heneicosylic acid (C21:0), and nervonic acid (C24:1). These results are in agreement with a previous study performed with alpha 2-heremans Schmid glycoprotein (AHSG) gene in Indonesian sheep population [31]. Monounsaturated FA including palmitoleic acid (C16:1) and nervonic acid (C24:1) have beneficial effects on health.…”

Section: Expression Analysis Of Olfml3 Genesupporting

confidence: 92%

Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep

et al. 2022

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Objective: The objective of this study was to identify polymorphism in olfactomedin like 3 (OLFML3) gene, and association analysis with meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep, and expression quantification of OLFML3 gene in phenotypically divergent sheep.Methods: A total of 328 rams at the age of 10 to 12 months with an average body weight of 26.13 kg were used. A novel polymorphism was identified using high-throughput sequencing in sheep and genotyping of OLFML3 polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 328 rams, 100 rams representing various sheep genotypes were used for association study and proc general linear model was used to analyse association between genotypes and phenotypic traits. Quantitative real-time polymerase chain reaction (qRT-PCR) was used for the expression analysis of OLFML3 mRNA in phenotypically divergent sheep population.Results: The findings revealed a novel polymorphism in the OLFML3 gene (g.90317673 C>T). The OLFML3 gene revealed three genotypes: CC, CT, and TT. The single nucleotide polymorphism (SNP) was found to be significantly (p<0.05) associated with meat quality traits such as tenderness and cooking loss; carcass characteristics such as carcass length; retail meat cut such as pelvic fat in leg, intramuscular fat in loin and tenderloin, muscle in flank and shank; fatty acids composition such as tridecanoic acid (C13:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), ginkgolic acid (C17:1), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), arachidonic acid (C20:4n6), heneicosylic acid (C21:0), and nervonic acid (C24:1). The TT genotype was associated with higher level of meat quality, carcass characteristics, retail meat cut, and some fatty acids composition. However, the mRNA expression analysis was not different among genotypes.Conclusion: The OLFML3 gene could be a potential putative candidate for selecting higher quality sheep meat, carcass characteristics, retail meat cuts, and fatty acid composition in sheep.

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Section: Expression Analysis Of Olfml3 Genesupporting

confidence: 92%

Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep

et al. 2022

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“…Fat in meat provides flavor, aroma and texture and are main factors that affect palatability of meat, thus affecting consumer acceptance (Maharani et al, 2012). Meat consumers dislike sheep meat due to off odour and flavour (Gunawan et al, 2018;Listyarini et al, 2018;Gunawan et al, 2019) but also complain for higher content in saturated fatty acids and cholesterol of sheep meat which are associated with cardiovascular diseases and some cancers (Kaić and Mioč 2016;Munyaneza et al, 2019). All saturated fatty acids (SFA) do not influence blood cholesterol at the same level (Sebsibe, 2008;Kaić and Mioč, 2016), myristic acid (C14:0) was the first saturated fatty acid to rise blood cholesterol level, palmitic acid (C16:0) comes next and lauric acid (C12:0), respectively (Sebsibe, 2008), the same author reported that myristic has four times the hypercholesterolemia action of other saturated fatty acids whereas all unsaturated fatty acids and stearic fatty acid are categorized as desirable fatty acids (DFA) (Frigolet and Gutiérrez-Aguilar, 2017).…”

Section: Introductionmentioning

confidence: 99%

Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep

Munyaneza

Gunawan

Noor

2019

J. Indonesian Trop. Anim. Agric.

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This study was aimed to explore the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in lambs. This study used a total of 147 blood samples for genotyping including 19 Javanese Fat-Tailed (JFT), 16 Javanese Thin-Tailed (JTT), 41 Composite Garut (CG), 35 Compass Agrinak (CA) and 36 Barbados Black Belly Cross (BC). A total of 61 rams as representative from five breed of sheep were selected for association study. Identification of BHMT single nucleotide polymorphisms was analyzed by PCR-RFLP method. Association of BHMT genotypes with fatty acid traits and cholesterol was performed by T-TEST. BHMT genotyping resulted into three genotypes (CC, CT and TT). Gene frequency of BHMT (g. 9947372 C>T) was in Hardy-Weinberg Equilibrium, excluding Javanese Fat-Tailed sheep. Association of BHMT genotypes with fatty acid traits resulted into a significant association (P<0.05) with C14:1, C17:1 and C18:0 fatty acids but not with cholesterol in sheep. SNP g. 9947372 (C>T) of BHMT gene might be a useful marker for selecting and producing sheep meat with desirable fatty acids.

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“…Several genes were reported to be significantly associated with fatty acid content in sheep including APOA5 (Gunawan et al, 2018b), AHSG (Munyaneza et al, 2019a), BHMT (Munyaneza et al, 2019b), DGAT1 (Gunawan et al, 2019), and KIF12 (Gunawan et al, 2018a). Cytochrome P450 2A6 (CYP2A6) are genes thought to contribute in the fatty acid traits in sheep.…”

Section: Introductionmentioning

confidence: 99%

Association of Cytochrome P450 2A6 (CYP2A6) Gene Polymorphisms with Fatty Acid Traits in Indonesian Native Sheep

et al. 2021

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A member of the cytochrome P450 superfamily enzyme that play a role in microsomal fatty acids oxidation process encoded by cytochrome P450 2A6 (CYP2A6). This study aimed to examined the CYP2A6 gene polymorphisms and their association on fatty acid traits in Indonesian native sheep. A total of 299 rams aged 10-12 months with body weights between 20-30 kg used to identify the CYP2A6 gene polymorphism are consisted of 36 samples of barbados cross sheep (BCS), 35 samples of compass agrinak sheep (CAS), 20 samples of javanese fat tailed sheep (JFT), 36 javanese thin tailed sheep (JTT), 20 samples of garut sheep (GS), 45 samples of garut composite sheep (GCS), meanwhile for the fatty acid analysis was carried out by using 107 of loin samples from the total sheep. Identification of the CYP2A6 gene polymorphisms were performed using PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) with the BSmAI restriction enzymes. The amplification product was 286 bp. Polymorphism were found in JFT, JTT, GCS and JS with GG and GT genotypes, while BCS, CAS, and GS were monomorphic with TT genotype. The CYP2A6 BSmAI polymorphism was in Hardy-Weinberg Equilibrium for JFT, JTT and JS, while BCS, CAS, GS, GCS, and combined was deviated based on chi square. A SNP g.49170107 G>T of the CYP2A6 gene polymorphism was significantly associated (P <0.05) with only erucic acid (C22:1n9). The GT genotypes had a higher value than GG genotypes The CYP2A6 gene could be used as a selection marker to improve fatty acid traits in Indonesian native sheep.

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Identification of Single Nucleotide Polymorphism and Association Analysis of Alpha 2-Heremans Schmid Glycoprotein (AHSG) Gene Related to Fatty Acid Traits in Sheep

Cited by 5 publications

References 16 publications

Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep

Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep

Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep

Association of Cytochrome P450 2A6 (CYP2A6) Gene Polymorphisms with Fatty Acid Traits in Indonesian Native Sheep

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