Identification of selenium compounds using HPLC-ICPMS and nano-ESI-MS in selenium-enriched rice via foliar application

Purification and identification of Se-containing antioxidative peptides from enzymatic hydrolysates of Se-enriched brown rice protein

“…SeMet was identified as the principal organic Se species in Se-BR. Previous research also reported that SeMet possesses approximately 87% of the total Se in Se-BR (Fang et al, 2009). However, all of the three selenoamino acids, namely, SeCys 2 , MeSeCys, and SeMet accounted for 44.3% of the total Se content in Se-BR in the present study.…”

“…Therefore, studies on Se-enriched products have been increasing over the recent decades. Se-enriched-yeasts (Ahmad et al, 2012), rice (Fang et al, 2009;Hu et al, 2014;Liu & Gu, 2009), mushroom (Maseko, Howell, Dunshea, & Ng, 2014), ovalbumin (Li et al, 2014), green tea (Li et al, 2008;Molan, 2013), and other materials have been recently reported.…”

Speciation of Organic and Inorganic Selenium in Selenium-enriched Eggs by Hydride Generation Atomic Fluorescence Spectrometry

“…However, there were few reports for the speciation of Se in Se-enriched foods, especially in Se-enriched eggs. The liquid chromatography ICP-MS had been used for the determination of selenospecies in Se-enriched rice with enzymatic hydrolysis extraction procedure (Fang et al 2009), and the Se-compounds in Se-enriched yeast and yeast-based tablets with accelerated solvent extraction (Li et al 2010). A hydride generationatomic fluorescence spectrometry(HG-AFS) with methylbenzene separation-microwave-assisted digestion was developed for the determination of organic and total selenium in selenium-enriched health food with the detection limit of 0.09 μg L −1 (Li et al 2008).…”

Selenium Biofortification in Rice - A pragmatic perspective

“…Accordingly, all these studies indicate that Semethionine is the major selenium species found in selenium enriched rice metabolized from inorganic selenium after foliar application. Therefore, selenium enriched rice can be used as a food supplement in addition to its use as a food staple in selenium deficient geographical areas (Fang et al, 2009). …”

“…further reported a 10 fold increase in selenium content of mature polished rice grain, following a single foliar spray application of 14-18 g ha -1 at the heading stage. Fang et al (2009) showed that foliar applications of 100 g ha -1 of selenite might led to 55 fold increase in the selenium content of rice grain (from 0.032 to 1.79 mg kg -1 ). Lyons et al (2004) further reported that the application of selenium is probably the most successful example of agronomic intervention by mineral fertilization, because selenate is highly mobile in many soil types, being easily absorbed by plants and accumulated in the grains in a bioavailable form (i.e., methionine and cysteine).…”