Identification of Redeye, a new sleep-regulating protein whose expression is modulated by sleep amount

Shi, Mi; Yue, Zhifeng; Kuryatov, Alexander; Lindstrom, Jon; Sehgal, Amita

doi:10.7554/elife.01473

Cited by 89 publications

(85 citation statements)

References 30 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Individual nAChR subunits also appear to have different roles in sleep regulation (Yi et al 2013;Shi et al 2014;Wu et al 2014). From this study, Da1 seems to have net sleep-promoting effects, especially with sleep maintenance.…”

Section: Activity and Sleepmentioning

confidence: 89%

“…melanogaster has 10 nAChR subunits; however, only three of these have been associated with behavioral phenotypes, with specific roles described for Da7 in the escape response (Fayyazuddin et al 2006) and for both Da3 and Da4 in sleep behavior (Shi et al 2014;Wu et al 2014). Mutations in three D. melanogaster nAChR subunits confer resistance to two important classes of insecticides; Da1 and Db2 to neonicotinoids (Perry et al 2008), and Da6 to spinosyns (Perry et al 2007Watson et al 2010;Somers et al 2015).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Pleiotropic Effects of Loss of the Dα1 Subunit in Drosophila melanogaster: Implications for Insecticide Resistance

et al. 2017

View full text Add to dashboard Cite

Nicotinic acetylcholine receptors (nAChRs) are a highly conserved gene family that form pentameric receptors involved in fast excitatory synaptic neurotransmission. The specific roles individual nAChR subunits perform in Drosophila melanogaster and other insects are relatively uncharacterized. Of the 10 D. melanogaster nAChR subunits, only three have described roles in behavioral pathways; Da3 and Da4 in sleep, and Da7 in the escape response. Other subunits have been associated with resistance to several classes of insecticides. In particular, our previous work has demonstrated that an allele of the Da1 subunit is associated with resistance to neonicotinoid insecticides. We used ends-out gene targeting to create a knockout of the Da1 gene to facilitate phenotypic analysis in a controlled genetic background. To our knowledge, this is the first report of a native function for any nAChR subunits known to be targeted by insecticides. Loss of Da1 function was associated with changes in courtship, sleep, longevity, and insecticide resistance. While acetylcholine signaling had previously been linked with mating behavior and reproduction in D. melanogaster, no specific nAChR subunit had been directly implicated. The role of Da1 in a number of behavioral phenotypes highlights the importance of understanding the biological roles of nAChRs and points to the fitness cost that may be associated with neonicotinoid resistance.

show abstract

Section: Activity and Sleepmentioning

confidence: 89%

mentioning

confidence: 99%

Pleiotropic Effects of Loss of the Dα1 Subunit in Drosophila melanogaster: Implications for Insecticide Resistance

et al. 2017

View full text Add to dashboard Cite

show abstract

“…A simple hypothesis, consistent with our finding that both activation of PL neurons and increased Cdk1 activity in these neurons suppress sleep, is that Cdk1 affects neuronal excitability and synaptic transmission. Interestingly, large-scale screens for short-sleeping mutants in fruit flies and zebrafish have identified several channel proteins such as SHAKER, REDEYE, and ETHER-A-GO-GO [6, 49, 50], and channel modulators such as SLEEPLESS and WIDE AWAKE [51, 52]. Thus it is plausible that Cdk1 regulates sleep by phosphorylating substrates that modulate the function of synaptic ion channels or proteins involved in synaptic vesicle fusion, as has previously been demonstrated for Cdk5 at mammalian synapses [53].…”

Section: Discussionmentioning

confidence: 99%

TARANIS Functions with Cyclin A and Cdk1 in a Novel Arousal Center to Control Sleep in Drosophila

et al. 2015

View full text Add to dashboard Cite

Sleep is an essential and conserved behavior, whose regulation at the molecular and anatomical level remains to be elucidated. Here we identify TARANIS (TARA), a Drosophila homolog of the Trip-Br (SERTAD) family of transcriptional coregulators, as a molecule that is required for normal sleep patterns. Through a forward-genetic screen, we isolated tara as a novel sleep gene associated with a marked reduction in sleep amount. Targeted knockdown of tara suggests that it functions in cholinergic neurons to promote sleep. tara encodes a conserved cell cycle protein that contains a Cyclin A (CycA)-binding homology domain. TARA regulates CycA protein levels and genetically and physically interacts with CycA to promote sleep. Further, decreased levels of Cyclin-dependent kinase 1 (Cdk1), a kinase partner of CycA, rescue the short-sleeping phenotype of tara and CycA mutants, while increased Cdk1 activity mimics the tara and CycA phenotypes, suggesting that Cdk1 mediates the role of TARA and CycA in sleep regulation. Finally, we describe a novel wake-promoting role for a cluster of ~14 CycA-expressing neurons in the pars lateralis (PL), previously proposed to be analogous to the mammalian hypothalamus. We propose that TARANIS controls sleep amount by regulating CycA protein levels and inhibiting Cdk1 activity in a novel arousal center.

show abstract

“…SSS also interacts with and suppresses the activity of nicotinic acetylcholine receptors to promote sleep (Wu et al 2014). On the other hand, the nicotinic acetylcholine receptor a subunit redeye (rye) interacts with SSS to promote sleep (Shi et al 2014), suggesting that cholinergic signaling can impact sleep in both directions. Levels of the RYE receptor are induced by both spontaneous and induced wakefulness (Shi et al 2014).…”

Section: Acetylcholinementioning

confidence: 99%

Molecular Mechanisms of Sleep Homeostasis in Flies and Mammals

Allada¹,

Cirelli

Sehgal

2017

Cold Spring Harb Perspect Biol

Self Cite

133

View full text Add to dashboard Cite

Identification of Redeye, a new sleep-regulating protein whose expression is modulated by sleep amount

Cited by 89 publications

References 30 publications

Pleiotropic Effects of Loss of the Dα1 Subunit in Drosophila melanogaster: Implications for Insecticide Resistance

Pleiotropic Effects of Loss of the Dα1 Subunit in Drosophila melanogaster: Implications for Insecticide Resistance

TARANIS Functions with Cyclin A and Cdk1 in a Novel Arousal Center to Control Sleep in Drosophila

Molecular Mechanisms of Sleep Homeostasis in Flies and Mammals

Contact Info

Product

Resources

About