Identification of quantitative trait loci (QTL) for plant structure, growth habit and yield in lentil

Abstract

Lentil quantitative trait loci (QTL) related to plant structure (branches at first node, height of first node, total number of branches, plant height), growth habit (flowering time, pod dehiscence) and yield (number of seeds, seed weight, seed diameter) were located using a F2 population of 113 individuals derived from the intersubspecific cross of Lens culinaris ssp. culinaris and L. c. ssp. orientalis. Several traits were found to be significantly correlated. Using interval and composite interval mapping a total of 23 QTL for nine quantitative traits were located. No QTL was identified for the number of F3 seed produced. Six QTL were positioned respectively in linkage groups III and VI, and five QTL in linkage group I. Each remaining group included one or two QTL, except groups VII and IX where no QTL was found. The multiple QTL model explained more than 80% of the observed phenotypic variance with logarithm of the odds (LOD) scores above 10 for three of the quantitative traits analyzed (branches at first node, flowering time, and dehiscence). For the remaining traits the phenotypic variance explained was relatively low, between the 50% and 20%, and the LOD scores ranged between 4 and 8. The possible homology between some QTL and other previously described is discussed in relation to their chromosomal location.

Morphological characterization and genetic diversity in lentil (Lens culinaris medikus ssp. Culinaris) germplasm

“…The breeders must have a mean of choosing the accession most likely to posses the trait of interest. Quantitative traits provide an estimate of genetic diversity and various numerical taxonomic techniques have been successfully used to classify and measure the pattern of phenotypic diversity in the relationship of germplasm collections in a variety of crops by many scientists as in lentil (Ahmad et al, 1997;Fratini et al, 2007;Tullu et al, 2008), Pea (Amurrio et al, 1995) and Alfalfa (Smith et al, 1991;Smith et al, 1995;Warburton and Smith, 1993). Worldwide, lentil is grown on a total of 1.8 million hectares, of which 60% is in the South Asian region, which includes the lentil producing countries of Bangladesh, Burma, India, Nepal and Pakistan (Nazir et al, 1994).…”

Short communication. An improved intersubspecific genetic map in Lens including functional markers

“…In order to maximize polymorphism for map construction in lentil, both, recombinant inbred lines and F 2 populations obtained from crosses between the cultivated lentil and the wild orientalis subspecies have been used to develop genetic maps in Lens (Havey & Muehlbauer, 1989;Eujayl et al, 1997Eujayl et al, , 1998Durán et al, 2004;Hamwieh et al, 2005), and as source of resistances (Pérez de la Vega et al, 2011). Several QTLs were further located in the intersubspecific map (Fratini et al, 2007) previously obtained by Durán et al (2004).…”

Assessment of genetic diversity in lentils (Lens culinaris Medik) based on single nucleotide polymorphisms

“…Economically, due to their highquality protein and fiber content, lentil seeds are an important food source for humans, especially in developing countries. Additionally, lentil straw is a valued animal feed (Fratini et al, 2007). In Palestine, lentil is an essential crop in the local agricultural system, and it is grown mainly under rain-fed conditions with a total production of about 170 tons obtained from 260 ha (FAO, 2013; http://faostat.fao.org).…”