Identification of processed Chinese medicinal materials using DNA mini-barcoding

Song, Ming; Gao, Dong; Zhang, Yaqin; Liu, Xia; Sun, Wei

doi:10.1016/s1875-5364(17)30073-0

Cited by 26 publications

(24 citation statements)

References 34 publications

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“…The results were consistent with a previously reported study that identi ed G. gecko from adulterants using COI regions [11,22]. DNA mini-barcoding and speci c-PCR identi cation are becoming more popular methods for effective species identi cation [24,25,31], especially for specimens with degraded DNA, such as archival specimens, processed foods, functional foods, and Chinese patent medicines [26,27,[32][33][34][35]. For a more sensitive identi cation of G. gecko in 2001, it was distinguished from 14 adulterants through allele-speci c diagnostic PCR which ampli ed 260-bp fragments of the 12S rRNA gene [36].…”

Section: Discussionsupporting

confidence: 91%

Specific DNA Mini-Barcoding for Identification of Gekko Gecko and Its Products

Ding

Yao

et al. 2020

Preprint

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Background: The dry body of the Tokay Gecko (Gekko gecko) is the source of a valuable traditional Chinese medicine, it is therefore listed as a Class II protected animal species in China. Due to increasing market demand and a declining supply of the species, a considerable number of adulterants have emerged in the market. Thus, it is necessary to establish an accurate and rapid method of identification for distinguishing G. gecko from its adulterants and for separating it from highly processed products.Methods: A total of 274 COI sequences were analyzed by using MEGA 5.0 software. Several specific primers were designed to amplify mini-barcode regions and identify G. gecko from its counterfeits and products.Results: 274 COI sequences of 16 G. gecko species and adulterants were analyzed. G. gecko could be distinguished from its adulterants through BLAST analysis, intra- and inter-specific distance analyses, and an NJ tree based on COI sequences. Two pairs of specific primers designed for this study, COISF2/COISR2 and COISF3/COISR3, amplified 200- and 133-bp fragments of the COI region, respectively, both of which were suitable for the identification of G. gecko and its adulterants. Furthermore, COISF3/COISR3 detected G. gecko in 15 batches of products.Conclusion: Therefore, the specific DNA mini-barcoding method developed here may be a powerful tool for the identification of G. gecko and counterfeits, and may also be used to distinguish G. gecko from its highly processed by-products.

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Section: Discussionsupporting

confidence: 91%

Specific DNA Mini-Barcoding for Identification of Gekko Gecko and Its Products

Ding

Yao

et al. 2020

Preprint

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“…And it is also possible that the samples were not suitable for ITS2 or psbA-trnH amplification. To tackle this issue, specific primers (Zhao et al, 2018) or other types of barcodes, such as minibarcodes (Song et al, 2017;Liu, 2018) or plastid superbarcodes (Krawczyk et al, 2018), can be used. However, this method also has limitations, according to our data, seven medicinal species were identified at the genus level, five exhibited low maximum similarity (<90%), and several could not be matched to any of the existing medicinal species, presumably due to the low species-level resolution of many plant genera and insufficient database information of GenBank.…”

Section: Resultsmentioning

confidence: 99%

Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding

Cui

Wei

et al. 2020

Front. Pharmacol.

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In Cambodia, medicinal plants are often used to treat various illnesses. However, the identities of many medicinal plants remain unknown. In this study, we collected 50 types of traditional Cambodian medicinal plants that could not be identified by their appearance from a domestic market. We utilized the DNA barcoding technique, combined with the literature survey, to trace their identities. In the end, 33 species were identified at the species level and 7 species were identified at the genus level. The ethnopharmacological information of 33 medicinal plants was documented. The DNA barcoding technique is useful in the identification of medicinal plants with no previous information.

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“…The results were consistent with a previously reported study that identified G. gecko from adulterants using COI regions [ 11 , 22 ]. DNA mini-barcoding and specific-PCR identification are becoming more popular methods for effective species identification [ 24 , 25 , 31 ], especially for specimens with degraded DNA, such as archival specimens, processed foods, functional foods, and Chinese patent medicines [ 26 , 27 , 32 – 35 ]. For a more sensitive identification of G. gecko in 2001, it was distinguished from 14 adulterants through allele-specific diagnostic PCR which amplified 260-bp fragments of the 12S rRNA gene [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Specific DNA mini-barcoding for identification of Gekko gecko and its products

Ding

Yao

et al. 2020

Chin Med

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Background The dry body of the Tokay Gecko (Gekko gecko) is the source of a valuable traditional Chinese medicine, it is therefore listed as a Class II protected animal species in China. Due to increasing market demand and a declining supply of the species, a considerable number of adulterants have emerged in the market. Thus, it is necessary to establish an accurate and rapid method of identification for distinguishing G. gecko from its adulterants and for separating it from highly processed products. Methods A total of 274 COI sequences were analyzed by using MEGA 5.0 software. Several specific primers were designed to amplify mini-barcode regions and identify G. gecko from its counterfeits and products. Results 274 COI sequences of G. gecko and 15 adulterants species were analyzed. G. gecko could be distinguished from its adulterants through BLAST analysis, intra- and inter-specific distance analyses, and an NJ tree based on COI sequences. Two pairs of specific primers designed for this study, COISF2/COISR2 and COISF3/COISR3, amplified 200- and 133-bp fragments of the COI region, respectively, both of which were suitable for the identification of G. gecko and its adulterants. Furthermore, COISF3/COISR3 detected G. gecko in 15 batches of products. Conclusion Therefore, the specific DNA mini-barcoding method developed here may be a powerful tool for the identification of G. gecko and counterfeits, and may also be used to distinguish G. gecko from its highly processed by-products.

show abstract

Identification of processed Chinese medicinal materials using DNA mini-barcoding

Cited by 26 publications

References 34 publications

Specific DNA Mini-Barcoding for Identification of Gekko Gecko and Its Products

Specific DNA Mini-Barcoding for Identification of Gekko Gecko and Its Products

Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding

Specific DNA mini-barcoding for identification of Gekko gecko and its products

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