Identification of Potential Markers for the Detection of Pancreatic Cancer Through Comparative Serum Protein Expression Profiling

Ehmann, Michael R.; Felix, Klaus; Hartmann, Daniel; Schnölzer, Martina; Nees, Matthias; Vorderwülbecke, Sonja; Bogumil, Ralf; Büchler, Markus W.; Friess, Helmut

doi:10.1097/01.mpa.0000250128.57026.b2

Cited by 136 publications

(107 citation statements)

References 52 publications

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“…In support of this, using serum protein profiling, several groups have recently reported increased serum levels of other apolipoproteins in patients with several types of cancer (Yu et al, 2005;Goncalves et al, 2006). On the other hand, Ehmann et al (2007) recently found that serum levels of different apolipoproteins (ApoA-1 and -2) were decreased in pancreatic cancer patients compared with healthy volunteers. These facts may indicate that several apolipoproteins have different roles in pancreatic cancer development.…”

Section: Apoc-1prevents Apoptosis In Pancreatic Cancer S Takano Et Almentioning

confidence: 86%

Apolipoprotein C-1 maintains cell survival by preventing from apoptosis in pancreatic cancer cells

et al. 2007

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Pancreatic cancer still remains one of the most lethal diseases and establishment of new therapy is needed. The purpose of this study is to find novel factors involved in pancreatic cancer progression by proteomic approach. We compared pre-and postoperative serum protein profiling obtained from pancreatic cancer patients who had curative pancreatectomy using surface-enhanced laser desorption ionization time-of-flight mass spectrometry. The peak intensity levels of both 6630 and 6420 Da were significantly higher in the preoperative serum than in the postoperative serum (Po0.002). Sequential amino acid analysis identified these proteins to be apolipoprotein C-1 (ApoC-1). The high level of ApoC-1 in preoperative serum significantly correlated with poor prognosis. Furthermore, ApoC-1 was abundantly expressed in pancreas neoplastic epithelium, and was detected in the culture medium of the pancreatic cancer cell line in vitro, which suggests that cancer cells secrete ApoC-1. Inhibition of ApoC-1 expression by short interfering RNA suppressed cell proliferation and induced apoptosis of pancreatic cancer cells. The specific expression of ApoC-1 and its role in preventing from spontaneous apoptosis in pancreatic cancer cells suggest that ApoC-1 contributes to the aggressiveness of pancreatic cancer and will be useful as a new therapeutic target.

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Section: Apoc-1prevents Apoptosis In Pancreatic Cancer S Takano Et Almentioning

confidence: 86%

Apolipoprotein C-1 maintains cell survival by preventing from apoptosis in pancreatic cancer cells

et al. 2007

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“…Whereas some studies used undefined protein extracts from tumor lysates (11,14,(28)(29)(30), others focused on limited numbers of candidate tumor antigens (24,25). To our knowledge, only two studies have performed a large-scale profiling of autoantibodies related to cancer using the extensive panel of human antigens present on ProtoArrays, one study probing with 12 colorectal cancer sera and 8 control sera (2), and the other probing with 30 ovarian cancer sera and 30 healthy controls (9).…”

Section: Discussionmentioning

confidence: 99%

Seromic profiling of ovarian and pancreatic cancer

Gnjatic

Ritter

Büchler³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Autoantibodies, a hallmark of both autoimmunity and cancer, represent an easily accessible surrogate for measuring adaptive immune responses to cancer. Sera can now be assayed for reactivity against thousands of proteins using microarrays, but there is no agreed-upon standard to analyze results. We developed a set of tailored quality control and normalization procedures based on ELISA validation to allow patient comparisons and determination of individual cutoffs for specificity and sensitivity. Sera from 60 patients with pancreatic cancer, 51 patients with ovarian cancer, and 53 age-matched healthy donors were used to assess the binding of IgG antibodies against a panel of >8000 human antigens using protein microarrays and fluorescence detection. The resulting data interpretation led to the definition and ranking of proteins with preferred recognition by the sera from cancer patients in comparison with healthy donors, both by frequency and strength of signal. We found that 202 proteins were preferentially immunogenic in ovarian cancer sera compared to 29 in pancreatic cancer, with few overlaps. Correlates of autoantibody signatures with known tumor expression of corresponding antigens, functional pathways, clinical stage, and outcome were examined. Serological analysis of arrays displaying the complete human proteome (seromics) represents a new era in cancer immunology, opening the way to defining the repertoire of the humoral immune response to cancer. serum antibody | biomarkers | protein microarrays | serology | autoantigen

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“…Overexpression of human apo A-I in transgenic mice have shown inhibition of tumour growth and improved survival rate. Decreased apo A-I levels have been reported in the serum of patients with pancreatic cancer, gastric cancer, and ovarian cancer [36][37][38]. Apo A-I along with apo A-11, transthyretin are found decreased by more than two fold in pancreatic cancers [36].…”

Section: Anti Tumor Activity Of Apo A-imentioning

confidence: 97%

“…Decreased apo A-I levels have been reported in the serum of patients with pancreatic cancer, gastric cancer, and ovarian cancer [36][37][38]. Apo A-I along with apo A-11, transthyretin are found decreased by more than two fold in pancreatic cancers [36]. Chong et al in their study of gastric cancers got varied expression of apo A-I in plasma in different size tumors.…”

Section: Anti Tumor Activity Of Apo A-imentioning

confidence: 97%

Apolipoprotein A-I: A Molecule of Diverse Function

2015

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Identification of Potential Markers for the Detection of Pancreatic Cancer Through Comparative Serum Protein Expression Profiling

Abstract: PCa is associated with a specific decrease of distinct serum proteins, which allows a reliable differentiation between pancreatic cancer and healthy controls.

Cited by 136 publications

References 52 publications

Apolipoprotein C-1 maintains cell survival by preventing from apoptosis in pancreatic cancer cells

Apolipoprotein C-1 maintains cell survival by preventing from apoptosis in pancreatic cancer cells

Seromic profiling of ovarian and pancreatic cancer

Apolipoprotein A-I: A Molecule of Diverse Function

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