Identification of potato Lipid transfer protein gene family and expression verification of drought genes StLTP1 and StLTP7

Abstract

Lipid transfer proteins (LTPs) are widely distributed in plants and play an important role in the response to stress. Potato (Solanum tuberosum L.) is sensitive to a lack of water, and drought stress is one of the limiting factors for its yield. Therefore, mining candidate functional genes for drought stress and creating new types of potato germplasm for drought resistance is an effective way to solve this problem. There are few reports on the LTP family in potato. In this study, 39 members of the potato LTP family were identified. They were located on seven chromosomes, and the amino acid sequences encoded ranged from 101 to 345 aa. All 39 family members contained introns and had exons that ranged from one to four. Conserved motif analysis of potato LTP transcription factors showed that 34 transcription factors contained Motif 2 and Motif 4, suggesting that they were conserved motifs of potato LTP.Compared with the LTP genes of homologous crops, the potato and tomato (Solanum lycopersicum L.) LTPs were the mostly closely related. The StLTP1 and StLTP7 genes were screened by quantitative reverse transcription PCR combined with potato transcriptome data to study their expression in tissues and the characteristics of their responses to drought stress. The results showed that StLTP1 and StLTP7 were upregulated in the roots, stems, and leaves after PEG 6000 stress. Taken together, our study provides comprehensive information on the potato LTP family that will help to develop a framework for further functional studies.expression analysis, genome-wide identification, LTP, potato) is one of the most important food crops and economic crops in China, and the amounts of its area and yield rank first in the world. Inner Mongolia is one of the primary bases for the production of potato seed and commercial potatoes in China. The annual rainfall is approximately 200-350 mm, and the region is arid and semiarid (du et al., 2013;Zhang, Li, et al., 2022). Drought stress is Dan Wang and Jian Song contributed equally to this work.

Porous borders at the wild-crop interface promote weed adaptation in Southeast Asia

“…In indica -like Thai weeds, for example, a candidate genomic region on chromosome 3 (26.0–26.1 Mb) contained several functionally important genes, such as lipid transfer protein (LTP) gene (LOC_Os03g46100 and LOC_Os03g46110) and F-box domain gene (LOC_Os03g46120 and LOC_Os03g46140). Plant LTP and F-box genes have been found to control many crucial cellular activities, including photomorphogenesis, floral meristem and floral organ identity determination, and response to abiotic stresses (i.e., drought, salt and cold) 50 , 51 . Likewise, a highly divergent genomic region (chromosome 10:11.4-11.6 Mb) was identified in Malaysian weeds.…”

Lipid transfer protein StLTPa enhances potato disease resistance against different pathogens by binding and disturbing the integrity of pathogens plasma membrane

“…nsLTPs bind to, and transport, various lipids, and have reported to participate in plant growth and development, seed development and germination and plant resistance to biotic and abiotic stresses (Gao et al ., 2022 ). For example, wheat ( Triticum aestivum ) TaLTP4 is produced in response to both biotic and abiotic stresses (Safi et al ., 2015 ), and potato StLTP1 and StLTP7 were reported to enhance potato drought stress tolerance (Wang et al ., 2023 ). Brassica rapa BrLTP2.1 shows antifungal activities (Schmitt et al ., 2018 ), whereas Arabidopsis thaliana AtLTP4.4 shows antifungal activity, and overexpression of AtLTP4.4 or its wheat homologue TaLTP3, increases wheat resistance to the pathogenic fungus Fusarium graminearum (McLaughlin et al ., 2021 ).…”

A Novel Non-Specific Lipid Transfer Protein Gene, CmnsLTP6.9, Enhanced Osmotic and Drought Tolerance by Regulating ROS Scavenging and Remodeling Lipid Profiles in Chinese Chestnut (Castanea mollissima Blume)

“…Lipids, as an important secondary metabolite, play a crucial role in plants' response to environmental stress, such as membrane stabilization, plant extracellular vesicle function, and cuticle and cell wall integrity [13][14][15][16]. In plants, considerable evidence shows that nsLTPs are involved in a range of lipid-related biological processes, including oil accumulation, cuticular wax deposition, pollen wall formation, and cutin synthesis [13,[17][18][19], while nsLTPs have also been found to respond to a variety of abiotic/biotic stresses, such as drought, high salinity, heat, cold stress, pathogen defense, PEG 6000 stress, and IAA and ABA stresses [20][21][22][23][24][25]. For abiotic stress, OsLTPL159 increased cold tolerance at the early seedling stage in rice [26].…”