Identification of PCR-based markers (RAPD, AFLP) linked to a novel powdery mildew resistance gene (ol-2) in tomato

Giovanni, Claudio De; Dell'Orco, P.; Bruno, Alfonso Sánchez; Ciccarese, F.; Lotti, Concetta; Ricciardi, Luigi M.

doi:10.1016/j.plantsci.2003.07.005

Cited by 48 publications

(38 citation statements)

References 30 publications

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“…All the ampliWcation products were subsequently digested with several restriction enzymes as described by Bai et al (2004). AFLP ® Wngerprints of the ol-2 linked markers identiWed by De Giovanni et al (2004) and Ricciardi et al (2007) were generated on a LICOR 4200 DNA sequencer as described by Bai et al (2003).…”

Section: Marker Analysismentioning

confidence: 99%

“…In addition, resistance conferred by the dominant Ol-genes is based on race-speciWc hypersensitive response (HR), thus is likely not durable. In contrast, the recessive gene ol-2, located around the centromere of chromosome 4 (De Giovanni et al 2004), derives from the germplasm of the cultivated tomato species, Solanum lycopersicum (Ciccarese et al 1998), and confers race-non-speciWc resistance via papilla formation, a non-HR-based mechanism (Bai et al 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Although marker assisted selection (MAS) with co-dominant molecular markers would allow the breeder to perform the selection of the ol-2 allele in backcross generations, the low genetic variation within S. lycopersicum (Miller and Tanksley 1990) hindered the identiWcation of polymorphic co-dominant markers linked to the resistance locus. So far, mainly ampliWed fragment length polymorphism (AFLP ® ) markers have been identiWed for the ol-2 region (De Giovanni et al 2004;Ricciardi et al 2007). In practical breeding, AFLP ® markers cannot oVer eYcient MAS as they are dominant and require advanced equipment.…”

Section: Introductionmentioning

confidence: 99%

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Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew

Pavan

Zhang²,

Borisova

et al. 2007

Euphytica

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The recessive gene ol-2 confers papillaassociated and race-non-speciWc resistance to tomato powdery mildew caused by Oidium neolycopersici. In order to facilitate marker assisted selection (MAS) in practical breeding programmes, we identiWed two simple sequence repeat (SSR) markers and one cleaved ampliWed polymorphic sequence (CAPS) marker which are linked to the resistance locus and co-dominantly inherited. Aiming to provide a base for ol-2 positional cloning, we used a large segregating F 2 population to merge these markers with all the ol-2 linked ampliWed fragment length polymorphism (AFLP ® ) markers previously identiWed in an integrated genetic map. By screening a tomato bacterial artiWcial chromosome (BAC) library, we detected two BAC clones containing two expressed sequence tags (ESTs) homologous to the gene mlo, responsible for powdery mildew resistance in barley, as well as an ol-2-linked marker. Chromosomal mapping by Fluorescence in situ Hybridization (FISH) revealed major signals of the two BAC DNAs in the pericentromeric heterochromatin of the short arm of chromosome 4, in the same region where the ol-2 gene was previously mapped. The genetic and cytogenetic co-localisation between ol-2 and tomato mlo-homologue(s), in addition to the similarity of ol-2 and mlo resistances for both genetic and phytopathological characteristics, suggests that ol-2 is likely a mlo-homologue. Thus, a homology-based cloning approach could be more suitable than positional cloning for ol-2 isolation.Keywords Fluorescence in situ hybridization · Homology-based cloning · Integrated linkage map · Map-based cloning · Oidium neolycopersici · Solanum lycopersicum

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Section: Marker Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew

Pavan

Zhang²,

Borisova

et al. 2007

Euphytica

Self Cite

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“…Molecular markers have provided a powerful tool for proper characterization of germplasm diversity (Williams et al, 1990). PCR-based molecular techniques provide a variety of DNA markers for diversity analysis (Newbury & Ford-Lloyd, 1993), identification of cultivars (Matsumoto & Fukui, 1996), gene localization, and construction of linkage maps (Giovanni et al, 2004). RAPD markers have been used for diversity analysis in cacao (Leal et al, 2008), banana (Souza et al, 2008) and, sugar apple (Bharad et al, 2009, Ahmad et al, 2010.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in sugar apple (Annona squamosa L.) by using RAPD markers

et al. 2013

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RESUMO Diversidade genética em pinheira (Annona squamosa L.) por meio de marcadores RAPDA diversidade genética da coleção de 64 acessos de pinheira, coletados em diferentes cidades, no norte do Estado de Minas Gerais, foi avaliada por meio do uso de marcadores RAPD. Foram selecionados 20 primers RAPD que geraram 167 fragmentos, dos quais 48 foram polimórficos (28,7%), produzindo uma média de 2,4 fragmentos polimórficos por primer. Baixa percentagem de polimorfismo foi obtida com o conjunto de primers (< 29%), indicando baixa variação genética entre os 64 acessos avaliados. As distâncias genéticas foram estimadas, utilizando-se o coeficiente de similaridade de Jaccard. Acessos de diferentes cidades foram agrupados em um mesmo grupo, indicando que não há correlação entre os agrupamentos moleculares e origem geográfica. O dendrograma revelou cinco grupos. O primeiro grupo reuniu os acessos C19 e G29, coletados nas cidades de Verdelândia e Monte Azul, respectivamente. O segundo grupo reuniu os acessos G16 e B11, coletados nas cidades de Monte Azul e Coração de Jesus, respectivamente. Os acessos remanescentes foram agrupados em três grupos, com oito, 15 e 37 acessos, respectivamente. O marcador RAPD apresentou baixo nível de polimorfismo entre os acessos avaliados. Genetic diversity in sugar apple (Annona squamosa L.) by using RAPD markers PalavrasGenetic diversity in a collection of 64 sugar apple accessions collected from different municipalities in northern Minas Gerais was assessed by RAPD analysis. Using 20 selected RAPD primers 167 fragments were generated, of which 48 were polymorphic (28.7%) producing an average of 2.4 polymorphic fragments per primer. Low percentage of polymorphism (< 29%) was observed by using the set of primers indicating low level of genetic variation among the 64 accessions evaluated. Genetic relationships were estimated using Jaccard's coefficient of similarity. Accessions from different municipalities clustered together indicating no correlation between molecular grouping and geographical origin. The dendrogram revealed five clusters. The first cluster grouped C19 and G29 accessions collected from the municipalities of Verdelândia and Monte Azul, respectively. The second cluster grouped G16 and B11 accessions collected from the municipalities of Monte Azul and Coração de Jesus, respectively. The remaining accessions were grouped in three clusters, with 8, 15 and 37 accessions, respectively. In summary, RAPD showed a low percentage of polymorphism in the germplasm collection.

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“…The AFLP technique combines the strengths of different marker systems and provides opportunities for mapping plants with large genomes and low polymorphism rates, such as wheat. Recently, several studies have described the association of AFLP markers with important resistance genes, such as greenbug and Fusarium head blight resistance in wheat (Weng and Lazar, 2002;Guo et al, 2003), powdery mildew resistance in tomato (De Giovanni et al, 2004), and SCMV and RppQ resistance in corn (Dussle et al, 2003;Chen et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi

Silva¹,

Brammer²,

Guerra³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Leaf rust is one of the most destructive diseases affecting wheat worldwide. The most effective way to control it is to use resistant cultivars. Resistance based on slow-rusting adult plant resistance (APR) genes has proven to be the best method for developing cultivars with durable resistance. A source of slow-rusting APR for leaf rust is the Brazilian wheat cultivar Toropi. The Toropi/IAC 13 F 2 and F 7 recombinant inbred lines (RILs) were developed in previous studies. Phenotypic analysis of the F 2 and F 7 RILs showed that 2 recessive genes that were temporarily named trp-1 and trp-2 conferred APR in Toropi. In the present study, we used monosomic families and amplified fragment length polymorphism (AFLP), sequence-tagged site, and simple sequence repeat (SSR) markers to map trp-1 and trp-2 on wheat chromosomes. Analysis of the F 2 monosomic RIL showed that trp-1 and trp-2 were located on chromosomes 1A and 4D, respectively. AFLP analysis of the F 7 RIL identified 2 independent AFLP markers, XPacgMcac3 and XPacgMcac6, which were associated with Toropi APR. These markers explained 71.5% of the variation in the phenotypic data in a multiple linear regression model. The AFLP markers XPacg/ Mcac3 and XPacg/Mcac6 were anchored by SSR markers previously mapped on the short arms of chromosomes 1A (1AS) and 4D (4DS), respectively. The trp-2 gene is the first leaf rust resistance gene mapped on wheat chromosome 4DS. The mapping of trp-1 and trp-2 provides novel and valuable information that could be used in future studies involving the fine mapping of these genes, as well as in the identification of molecular markers that are closely related to these genes for markerassisted selection of this important trait in wheat.

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Identification of PCR-based markers (RAPD, AFLP) linked to a novel powdery mildew resistance gene (ol-2) in tomato

Cited by 48 publications

References 30 publications

Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew

Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew

Genetic diversity in sugar apple (Annona squamosa L.) by using RAPD markers

Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi

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