Identification of nucleotide preferences in DNA sequences recognised specifically by c-Ets-1 protein

Abstract

ABSTRACT

ETS1 lowers capillary endothelial cell density at confluence and induces the expression of VE-cadherin

“…Regarding Ets1 speci®city of binding to DNA, our EMSA results are in accordance with what was already known on the structure of the Ets1 binding site (Fisher et al, 1991;Ghysdael and Boureux, 1997;Woods et al, 1992). rEts1 bound to EBS2 and EBS4 but failed to bind to EBS1, EBS3 or EBS5, although they had the correct GGAA/T core sequence.…”

“…rEts1 bound to EBS2 and EBS4 but failed to bind to EBS1, EBS3 or EBS5, although they had the correct GGAA/T core sequence. These results support the previous observations that an A in position 73 relative to the ®rst G of the core sequence and a G in position +5 favor binding of Ets1 to DNA (Fisher et al, 1991;Nye et al, 1992;Woods et al, 1992).…”

A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer

“…ELK1 related SAP1 binds weakly to GGAT core binding sites, but both ELK1 and SAP1 require ACC residues¯anking one side of the core (positions 75, 74 and 73, in the ETS1 consensus) and prefer GT residues anking the other side (positions +3 and +4) to achieve binding to GGAT containing sites. It is interesting to note that these speci®c¯anking sequences, as present in the E74 oligonucleotide, confer very high a nity binding for ETS1 (Nye et al, 1992;Woods et al, 1992). FLI1 also contains the GGAT binding-associated lysine residue, but appears to bind GGAT sequences very rarely (1 in 22 binding sites tested) (Mao et al, 1994), and again only in the context of the highest a nity¯anking sequences.…”

“…FLI1 also contains the GGAT binding-associated lysine residue, but appears to bind GGAT sequences very rarely (1 in 22 binding sites tested) (Mao et al, 1994), and again only in the context of the highest a nity¯anking sequences. The situation of ETS1 binding to GGAT sequences is di erent, where a wide range of¯anking sequences are permissible (Nye et al, 1992;Woods et al, 1992). Thus, ETS1 can bind GGAT core sequences, even when the¯anking sequences specify low a nity binding, and other ETS family members appear to bind GGAT core sequences:…”

Roles of an Ets motif and a novel CACGAC direct repeat in transcription of the murine dihydrolipoamide dehydrogenase (Dld) gene

“…The Ets family has a highly conserved C-terminal DNA-binding domain, which recognizes a GGA motif. It has been noted that different Ets family members have different DNA binding specificities [34][35][36]. For example, an Ets motif in the nicotinic acetylcholine receptor promoter binds with GABP but not with either Ets-2 or Elf-1 in EMSAs [37].…”