Identification of novel reassortant mammalian orthoreoviruses from bats in Slovenia

Naglič, Tina; Rihtarič, Danijela; Hostnik, Peter; Toplak, Nataša; Koren, Simon; Kuhar, Urška; Jamnikar-Ciglenečki, Urška; Kutnjak, Denis; Steyer, Andrej

doi:10.1186/s12917-018-1585-y

Cited by 27 publications

(16 citation statements)

References 21 publications

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“…Earlier studies associated a conserved Arg-Gly-Asp ( 167 RGD 171 ) motif on the T13 protein of BTV with the attachment to Culicoides cells [90,91]. This conserved motif was also found by later studies in some species closely related to BTV [43,48,70], but we did not observed it in LEAV. As with other orbivirus species, this may reflect the higher divergence relative to BTV.…”

Section: Discussionsupporting

confidence: 65%

See 1 more Smart Citation

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Tomazatos

Marschang

Maranda

et al. 2020

Viruses

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The discovery and characterization of novel arthropod-borne viruses provide valuable information on their genetic diversity, ecology, evolution and potential to threaten animal or public health. Arbovirus surveillance is not conducted regularly in Romania, being particularly very scarce in the remote and diverse areas like the Danube Delta. Here we describe the detection and genetic characterization of a novel orbivirus (Reoviridae: Orbivirus) designated as Letea virus, which was found in grass snakes (Natrix natrix) during a metagenomic and metatranscriptomic survey conducted between 2014 and 2017. This virus is the first orbivirus discovered in reptiles. Phylogenetic analyses placed Letea virus as a highly divergent species in the Culicoides-/sand fly-borne orbivirus clade. Gene reassortment and intragenic recombination were detected in the majority of the nine Letea virus strains obtained, implying that these mechanisms play important roles in the evolution and diversification of the virus. However, the screening of arthropods, including Culicoides biting midges collected within the same surveillance program, tested negative for Letea virus infection and could not confirm the arthropod vector of the virus. The study provided complete genome sequences for nine Letea virus strains and new information about orbivirus diversity, host range, ecology and evolution. The phylogenetic associations warrant further screening of arthropods, as well as sustained surveillance efforts for elucidation of Letea virus natural cycle and possible implications for animal and human health.

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Section: Discussionsupporting

confidence: 65%

“…This is indicated by detections of reassortment and intragenic recombination in eight, respectively seven, of nine LEAV strains. This is all the more clear for orbiviruses with great antigenic diversity like BTV [57,66,67] and AHSV [68], but also for orthoreoviruses [69][70][71] and rotaviruses [72].…”

Section: Discussionmentioning

confidence: 99%

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Tomazatos

Marschang

Maranda

et al. 2020

Viruses

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“…The serotype specificity is determined by σ1 protein encoded in the S1 segment, which has the largest sequence divergence [12][13][14]. The genome segments of MRV have been exchanged between evolutionarily distinct strains, resulting in unpredicted reassortant viruses containing various combinations of the genome segments [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…MRVs have been identified in humans, livestock and wild animals with a wide host range [2,[19][20][21][22][23][24][25][26]. Individual MRVs can infect multiple species; several interspecies transmission and genetic reassortment events involving both humans and animals have been reported [2,[15][16][17][18][19][26][27][28]. An MRV strain isolated from a child with acute gastroenteritis had high similarity with MRVs detected in bats, indicating interspecies transmission between bats and humans [26].…”

Section: Introductionmentioning

confidence: 99%

Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

Harima

Sasaki

Kajihara

et al. 2020

Journal of General Virology

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Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.

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“…Tina Mikuletič (University of Ljubljana) discovered all 3 orthoreovirus serotypes and various reassortants thereof in Slowenian bat populations (10) and showed that bat orthoreoviruses can spread systemically in newborn mice.…”

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confidence: 99%

The 13th International Double-Stranded RNA Virus Symposium, Houffalize, Belgium, 24 to 28 September 2018

Desselberger

2019

J Virol

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The triennial International Double-Stranded RNA Virus Symposium, this year organized by J. Matthijnssens, J. S. L. Parker, P. Danthi, and P. Van Damme in Belgium, gathered over 200 scientists to discuss novel observations and hypotheses in the field. The keynote lecture on functional interactions of bacteria and viruses in the gut microbiome was presented by Julie Pfeiffer. Workshops were held on viral diversity, molecular epidemiology, molecular virology, immunity and pathogenesis, virus structure, the viral use and abuse of cellular pathways, and applied doublestranded RNA (dsRNA) virology. The establishment of a plasmid only-based reverse genetics system for rotaviruses by several Japanese research groups in 2017 has now been reproduced by various other research groups and was discussed in detail. The visualization of dsRNA virus replication steps in living cells received much attention. Mechanisms of the cellular innate immune response to virus infection and of viral pathogenesis were explored. Knowledge of the gut microbiome's influence on specific immune responses has increased rapidly, also due to the availability of relevant animal models of virus infection. The method of cryo-electron microscopic (cryo-EM) tomography has elucidated various asymmetric structures in viral particles. The use of orthoreoviruses for oncolytic virotherapy was critically assessed. The application of llama-derived single chain nanobodies for passive immunotherapy was considered attractive. In a satellite symposium the introduction, impact and further developments of rotavirus vaccines were reviewed. The Jean Cohen Lecturer of this meeting was Harry B. Greenberg, who presented aspects of his research on rotaviruses over a period of more than 40 years. He was also interviewed at the meeting by Vincent Racaniello for the 513th session of This Week in Virology.

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Identification of novel reassortant mammalian orthoreoviruses from bats in Slovenia

Cited by 27 publications

References 21 publications

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

The 13th International Double-Stranded RNA Virus Symposium, Houffalize, Belgium, 24 to 28 September 2018

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