Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients

Plana, Emma; Gálvez, Laura; Medina, Pilar; Navarro, Silvia; Fornés-Ferrer, Victoria; Panadero, Joaquín; Miralles, Manuel

doi:10.3390/ijms21134600

Cited by 23 publications

(26 citation statements)

References 69 publications

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“…When comparing data on patients with PA with our previously published article on AAA patients, 7 we can observe that eight of the 14 miRs were changed in different ways in the subgroups of patients with PA. Six of the 14 miRs (miR-33a, miR-103a, miR-125a, miR-192, miR-215, and miR-451) were altered in patients with PA with concomitant AAA. Among the miRs, miR-103a has previously been identified among AAA patients in a study by Plana et al, 13 as being downregulated 1.4-fold in plasma of AAA patients when unadjusted for covariates and upregulated 1.3-fold in aortic tissue from AAA patients. This miR was slightly upregulated in plasma from AAA patients in our previous study.…”

Section: Discussionmentioning

confidence: 95%

Circulating microRNA in patients with popliteal and multiple artery aneurysms

Wågsäter

Ravn

Wanhainen

et al. 2021

JVS-Vascular Science

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Background Patients with popliteal artery aneurysm (PA) often have multiple aneurysms, such as bilateral disease or a concomitant abdominal aortic aneurysm (AAA). microRNAs (miRs) are regulators of biological processes and have been investigated as biomarkers for AAA. The aim of this study was to explore if the presence of multiple aneurysms and/or location correlated with miR levels in blood. Methods Using quantitative polymerase chain reaction, 23 miRs were analyzed in plasma from 183 patients with PA. Results Fifteen of the miRs were associated with the number and/or location of aneurysms (1.3- to 2.1-fold changes). Levels of miR-93 (1.4-fold) and miR-215 (1.6- to 1.9-fold) were changed in all compared groups. MiR-24 and miR-23a were altered in those with AAA (1.4- and 1.5-fold, respectively) or bilateral PA (1.5- and 1.4-fold, respectively), compared with in those without. MiR-145 were significantly altered (1.7-fold) in those with isolated PA and AAA, whereas miR-326 were altered in those with bilateral (2.3-fold) and isolated PA (1.9-fold). Conclusions Different miRs seem to be important or to be markers for different subgroups of patients with PA. The identified miRs target vascular smooth muscle cell proliferation and vascular inflammation. Further studies are needed to increase the understanding of the pathogenesis of aneurysmal disease.

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Section: Discussionmentioning

confidence: 95%

Circulating microRNA in patients with popliteal and multiple artery aneurysms

Wågsäter

Ravn

Wanhainen

et al. 2021

JVS-Vascular Science

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“… 61 Another study also confirmed that THBS2 expression was increased in abdominal aortic aneurysm tissue, and further found that THBS2 could act as a potential target of miR-195-5p , which may be involved in the formation of abdominal aortic aneurysm. 55 We found that THBS2 was highly expressed in the tissues of IA. This is similar to the results of the various previous studies, which prove that THBS2 may be involved in the formation of aneurysms, but the specific mechanism needs to be further studied.…”

Section: Discussionmentioning

confidence: 74%

“…THBS2 is a multifunctional stromal cell protein encoded by the THBS2 gene and belongs to the thrombinsensitive protein family. 55 It has been reported to be involved in the regulation of angiogenesis, proliferation, apoptosis, tumor migration, autophagy and transforming growth factor (TGF) β activation. [56][57][58][59] In arteries, THBS2 is mainly secreted by the smooth muscle cells and partly by endothelial cells.…”

Section: Dovepressmentioning

confidence: 99%

Identification of Hub Genes Associated with the Pathogenesis of Intracranial Aneurysm via Integrated Bioinformatics Analysis

Zhong¹,

Ding²,

Zhou³

et al. 2021

IJGM

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Background At present, the pathogenesis of intracranial aneurysms (IA) remains unclear, which significantly hinders the development of novel strategies for the clinical treatment. In this study, bioinformatics methods were used to identify the potential hub genes and pathways associated with the pathogenesis of IA. Methods The gene expression datasets of patients with intracranial aneurysm were downloaded from the Gene Expression Database (GEO), and the different data sets were integrated by the robust rank aggregation (RRA) method to identify the differentially expressed genes between patients with intracranial aneurysm and the controls. The functional enrichment analyses of the significant differentially expressed genes (DEGs) were performed and the protein–protein interaction (PPI) network was constructed; thereafter, the hub genes were screened by cytoHubba plug-in of Cytoscape, and finally sequencing dataset GSE122897 was used to verify the hub genes. Results The GSE15629, GSE75436, GSE26969, and GSE6551 expression profiles have been included in this study, including 34 intracranial aneurysm samples and 26 control samples. The four datasets obtained 136 significant DEGs (45 up-regulated, 91 down-regulated). Enrichment analysis showed that the extracellular matrix structural constituent and the ECM-receptor interaction were closely related to the occurrence of IA. It was finally determined that eight hub genes associated with the development of IA, including VCAN, COL1A1, COL11A1, COL5A1, COL5A2, POSTN, THBS2 , and CDH2 . Conclusion The discovery of potential hub genes and pathways could enhance the understanding of the molecular mechanisms associated with the development of IA. These hub genes may be potential therapeutic targets for the management and new biomarker for the diagnosis of IA.

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“…Multiple types of PVADSCs can be induced by the transfection of microRNA (miRNA) mimics ( 72 ). Based on gene set enrichment analysis, the respective expression levels of miR-27b-3p and miR-221-3p in plasma were 1.6-fold and 1.9-fold higher in patients with AAAs than in healthy controls ( 73 ). Additionally, miR-221-3p is highly expressed in obese PVAT-derived extracellular vesicles (EVs).…”

Section: Cellular and Molecular Contact Between Dysfunctional Pvat And Aaa Pathologymentioning

confidence: 99%

Relationships Between Perivascular Adipose Tissue and Abdominal Aortic Aneurysms

Zhang

Liu

et al. 2021

Front. Endocrinol.

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Abdominal aortic aneurysms (AAAs) are typically asymptomatic, and there is a high mortality rate associated with aneurysm rupture. AAA pathogenesis involves extracellular matrix degradation, vascular smooth muscle cell phenotype switching, inflammation, and oxidative stress. There is increasing evidence of excessive adipocyte accumulation in ruptured AAA walls. These excessive numbers of adipocytes in the vascular wall have been closely linked with AAA progression. Perivascular adipose tissue (PVAT), a unique type of adipose tissue, can be involved in adipocyte accumulation in the AAA wall. PVAT produces various chemokines and adipocytokines around vessels to maintain vascular homeostasis through paracrine and autocrine mechanisms in normal physiological conditions. Nevertheless, PVAT loses its normal function and promotes the progression of vascular diseases in pathological conditions. There is evidence of significantly reduced AAA diameter in vessel walls of removed PVAT. There is a need to highlight the critical roles of cytokines, cells, and microRNA derived from PVAT in the regulation of AAA development. PVAT may constitute an important therapeutic target for the prevention and treatment of AAAs. In this review, we discuss the relationship between PVAT and AAA development; we also highlight the potential for PVAT-derived factors to serve as a therapeutic target in the treatment of AAAs.

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Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients

Cited by 23 publications

References 69 publications

Circulating microRNA in patients with popliteal and multiple artery aneurysms

Circulating microRNA in patients with popliteal and multiple artery aneurysms

Identification of Hub Genes Associated with the Pathogenesis of Intracranial Aneurysm via Integrated Bioinformatics Analysis

Relationships Between Perivascular Adipose Tissue and Abdominal Aortic Aneurysms

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