Identification of Nontuberculous Mycobacteria in Drinking Water in Cali, Colombia

Dávalos, Andrés F.; Garcia, Pamela K.; Montoya-Pachongo, Carolina; Rengifo, Andrea; Guerrero, Daniela; Díaz-Ordóñez, Lorena; Díaz, Gustavo; Ferro, Beatriz E.

doi:10.3390/ijerph18168451

Cited by 12 publications

(5 citation statements)

References 54 publications

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“…In a study by Davalos et al, 100% (2/2) of M. chelonae strains were sensitive to clarithromycin. However, one strain (25%) of M. mucogenicum resistant to this antibiotic was detected [ 46 ]. In our study, two strains representing M. mucogenicum were sensitive to most of the tested antibiotics.…”

Section: Discussionmentioning

confidence: 99%

New RAPMYCOI SensititreTM Antimicrobial Susceptibility Test for Atypical Rapidly Growing Mycobacteria (RGM)

Borek¹,

Zabost²,

Głogowska³

et al. 2022

Diagnostics

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Rapidly growing mycobacteria (RGM) cause an increasing international concern, mainly due to their natural resistance to many antibiotics. The aim of this study was to conduct species identification and determine the antimicrobial susceptibility profiles of RGM isolated in Poland. Antimicrobial susceptibility was tested using broth microdilution and the RAPMYCOI panel. A total of 60 strains were analysed, including the following species: M. fortuitum complex (30), M. abscessus subsp. abscessus (16), M. abscessus subsp. massiliense (7), M. chelonae (5), and M. mucogenicum (2). For 12 M. abscessus subsp. abscessus strains, the presence of the erm 41T28 genotype associated with inducible macrolide resistance and a functional erm gene was confirmed. A MUT2 mutation in the rrl gene (constitutive resistance) was identified for two strains from the subtype M. abscessus subsp. massiliense. Among the 15 tested antibiotics, amikacin and linezolid had the strongest antimycobacterial activity. Most of the tested strains were resistant to doxycycline and trimethoprim/sulfamethoxazole. Tigecycline MICs were low for all tested strains. Findings from our study highlight the importance of correct identification of clinical isolates and antimicrobial susceptibility testing.

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Section: Discussionmentioning

confidence: 99%

New RAPMYCOI SensititreTM Antimicrobial Susceptibility Test for Atypical Rapidly Growing Mycobacteria (RGM)

Borek¹,

Zabost²,

Głogowska³

et al. 2022

Diagnostics

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“…This finding is partly due to exposures in workplaces and institutions that led to respiratory diseases that were previously undiagnosed. Undoubtedly, as epidemiology and surveillance methods advance, more species will appear as water-related illnesses [ 23 , 26 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation of nontuberculous mycobacteria species from different water sources: a study of six hospitals in Tehran, Iran

et al. 2022

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Purpose Nontuberculous mycobacteria (NTM) are ubiquitous bacteria that are naturally resistant to disinfectants and antibiotics and can colonize systems for supplying drinking water. Therefore, this study aimed to evaluate the prevalence of NTM in the drinking water of six hospitals in Tehran, Iran. Methods Totally, 198 water samples were collected. Each water sample was filtered via a membrane filter with a pore size of 0.45 µm and then decontaminated by 0.005% cetylpyridinium chloride. The membrane filters were incubated on two Lowenstein-Jensen media at 25 °C and 37 °C for 8 weeks. The positive cultures were identified with phenotypic tests, and then NTM species were detected according to the hsp65, rpoB, and 16S rDNA genes. Drug susceptibility testing (DST) was also carried out. Results Overall, 76 (40.4%) of the isolates were slowly growing mycobacteria (SGM) and 112 (59.6%) of the ones were rapidly growing mycobacteria (RGM). The most common NTM were Mycobacterium aurum, M. gordonae, M. phocaicum, M. mucogenicum, M. kansasii, M. simiae, M. gadium, M. lentiflavum, M. fortuitum, and M. porcinum. Among these 188 samples, NTM ranged from 1 to > 300 colony-forming unit (CFU) /500 mL, with a median of 182 CFU/500 mL. In the infectious department of all hospitals, the amount of CFU was higher than in other parts of the hospitals. The DST findings in this study indicated the diversity of resistance to different drugs. Among RGM, M. mucogenicum was the most susceptible isolate; however, M. fortuitum showed a different resistance pattern. Also, among SGM isolates, M. kansasii and M. simiae, the diversity of DST indicated. Conclusions The current study showed NTM strains could be an important component of hospital water supplies and a possible source of nosocomial infections according to the CFU reported in this study. The obtained findings also help clarify the dynamics of NTM variety and distribution in the water systems of hospitals in the research area.

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“…Se ha descrito el hallazgo de micobacterias en fuentes de agua y suelo; en este caso se puede comparar la actividad antimicrobiana del AU encontrado en las especies liquénicas de U. steineri y E. prunastri frente a M. tuberculosis, con resultados de MIC de 8 μg/mL y 16 μg/mL, respectivamente. De manera análoga, se obtienen resultados del AU contra NTM, como M. avium de 16 μg/mL (MIC) frente al AU de E. prunastri y contra M. kansasii 8 μg/mL (MIC), con AU proveniente de U. steineri [39], [47], [50].…”

Section: Mecanismos De Acción Y Actividad Antimicrobiana Del Auunclassified

Artículo de revisión. Ácido úsnico: alternativa potencial contra la resistencia bacteriana actual

Avila-Zamora,

Pinzón-Pérez,

Acero-Godoy

2023

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El ácido úsnico (AU) es una sustancia liquénica, que hace parte de los metabolitos secundarios de los líquenes, los cuales son organismos que generan una simbiosis entre un organismo fotosintético y un micobionte, como ejemplo Usnea sp, producidos por diferentes rutas metabólicas, como es el caso del AU sustancia resultante de la vía del acetato-malonato. Por otro lado, la extracción de AU se realiza por medio de solventes orgánicos, maceración y para su identificación se realiza cromatografía líquida de alta eficiencia (HPCL). Las sustancias liquénicas cuentan con características antimicóticas, antivirales, citotóxicas, antimicrobianas, esta última ha sido estudiada contra bacterias patógenas como Staphylococcus sp., Escherichia coli, Streptoccocus sp., entre otros, por considerarse de prevalencia hospitalaria, debido a que han desarrollado resistencia contra los antibióticos comúnmente usados, generando preocupación; por lo que se buscan nuevas alternativas que permitan seguir combatiendo a estos patógenos, se han investigado los mecanismos de acción utilizados por el AU, entre los que se encuentra, la capacidad de inhibir la síntesis de primers bacterianos evitando su replicación, en la literatura ya se ha confirmado su uso a nivel in vitro contra bacterias multirresistentes como en Staphylococcus aureus MRSA demostrando que el AU presenta una concentración mínima inhibitoria (MIC) de 25–50 μg/mL [1]. El mecanismo de inhibición del AU puede generar detención del crecimiento bacteriano; por medio de mecanismos como inhibición de la replicación (ADN-ARN) además de la inhibición genes de resistencia bacteriana blindando así este posible antibiótico frente a cepas bacterianas resistentes encontradas actualmente.

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Identification of Nontuberculous Mycobacteria in Drinking Water in Cali, Colombia

Cited by 12 publications

References 54 publications

New RAPMYCOI SensititreTM Antimicrobial Susceptibility Test for Atypical Rapidly Growing Mycobacteria (RGM)

New RAPMYCOI SensititreTM Antimicrobial Susceptibility Test for Atypical Rapidly Growing Mycobacteria (RGM)

Isolation of nontuberculous mycobacteria species from different water sources: a study of six hospitals in Tehran, Iran

Artículo de revisión. Ácido úsnico: alternativa potencial contra la resistencia bacteriana actual

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