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Cited by 28 publications
(37 citation statements)
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“…In both the first and the second nested PCR, several samples tested negative. A false-negative PCR may be caused by the time between collection and testing of the specimen, the absence of bacilli in the specimen tested, or technical limitations (5,6,10,19). Taken together, the first and second nested PCRs detected 100% of samples from the 21 cultureconfirmed patients.…”
Section: Discussionmentioning
confidence: 97%
See 1 more Smart Citation
“…In both the first and the second nested PCR, several samples tested negative. A false-negative PCR may be caused by the time between collection and testing of the specimen, the absence of bacilli in the specimen tested, or technical limitations (5,6,10,19). Taken together, the first and second nested PCRs detected 100% of samples from the 21 cultureconfirmed patients.…”
Section: Discussionmentioning
confidence: 97%
“…For the diagnosis of M. leprae infections, PCR can be performed on sections of 5 m of frozen or paraffin-embedded punch skin biopsy samples (6). For the identification of mycobacterial DNA in cutaneous lesions of sarcoidosis, 15 to 20 sections of 5 m of punch biopsy samples have been used (10). Punch biopsies can be used for diagnosis of cutaneous tuberculosis with PCR dot blotting since both culture and histopathological examination are difficult due to the paucity of organisms (2).…”
Section: Discussionmentioning
confidence: 99%
“…The depth of the biopsy specimen is important, since both the typical necrosis of M. ulcerans disease and AFB are seen in deep dermis and subcutaneous fat. Punch biopsy specimens have been used to diagnose several dermatological conditions, including carcinoma, dermatofibroma, sarcoidosis (11), and cutaneous tuberculosis (1). When Todd et al (23) evaluated 2-mm punch biopsy specimens from patients with dermatological diseases, the diagnostic accuracy compared favorably with that obtained with the standard ellipse biopsy specimen, with an overall concordance rate of 94%, but this diameter has been found to be too small for M. ulcerans lesions.…”
Section: Discussionmentioning
confidence: 99%
“…Various infectious agents have been suggested as being involved, of which mycobacteria seem to be the most important. Even though a considerable number of investigators have failed to detect mycobacteria in clinical samples from patients with sarcoidosis (28,33), several studies have suggested the exclusive involvement of Mycobacterium avium complex members in sarcoidosis (8,13,20,24,25), while other studies have proposed Mycobacterium tuberculosis complex to be responsible (9,22,27,29) (Table 1). Therefore, a convincing association between sarcoidosis and mycobacterial infections has yet to be established.…”
mentioning
confidence: 99%